Preparation method of zein microsphere and ultrasonic built-in dialysis device for preparing zein microsphere

Abstract

The invention discloses a preparation method of a zein microsphere and an ultrasonic built-in dialysis device for preparing the zein microsphere. A dialysis bag of the device is positioned in a jacket kettle filled with a dialysis solution; the jacket kettle is connected with a constant-temperature tank; and an ultrasonic generator is connected with an ultrasonic transducer, the top of an ultrasonic amplitude transformer is connected with the ultrasonic transducer, the ultrasonic amplitude transformer extends into the dialysis bag and is fixed with the upper end of the dialysis bag, and the bottom of the ultrasonic amplitude transformer is positioned at the middle part of the dialysis solution in the dialysis bag. The method comprises the following steps: dissolving zein into an ethanol solution, placing a zein solution in the dialysis bag, putting the fixed dialysis bag into the dialysis solution, opening the ultrasonic generator, and controlling the ultrasonic power to be 150-225W; and performing freeze drying on a product obtained after dialysis to prepare the product. The zein microsphere obtained by using an ultrasonic built-in dialysis process disclosed by the invention is smooth in surface, has the particle size of 1-2 microns, is narrow in particle size distribution and good in dispersion performance, and can be used for oral administration and suction-type administration or can be prepared into a suspension for injection.

Description

Technical field [0001] The invention relates to a zein, in particular to a preparation method of zein microspheres and an ultrasonic built-in dialysis device for preparation. Background technique [0002] Microspheres are a new type of drug formulation with great development potential. The microsphere preparation can not only mask the bad smell of the drug, improve the compliance of the patient's medication, but also adjust and control the release rate of the drug, protect the drug from degradation by enzymes in the body, reduce the number of administrations and drug stimulation, and improve the efficacy . In addition, microspheres also have a special affinity with certain cell tissues. They can be absorbed by the reticuloendothelial system of organ tissues or fused by cells, concentrated in the target area and gradually diffuse to release drugs or be degraded by enzymes in lysosomes. The drug is released, which acts as a targeted therapy. With its unique advantages, microsphe...

AI Technical Summary

Problems solved by technology

However, zein is a large protein molecule, the self-assembly process is greatly affected by operating parameters, and zein microspheres are prone to agglomeration without external force

Directly using dialysis to prepare zein microspheres has defects such as uneven particle size, uncontrollable particle size, serious agglomeration, and poor dispersion.

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