Preparation method of zein microspheres and ultrasonic built-in dialysis device for preparation
A technology of zein and dialysis equipment, which is applied in the direction of microcapsules, nanocapsules, non-active components of polymer compounds, etc., and can solve the problems of large influence of operating parameters, uncontrollable particle size, and uneven particle size , to achieve the effect of increasing the dialysis rate, avoiding local nucleation, and narrowing the particle size distribution
Active Publication Date: 2017-05-03
SOUTH CHINA UNIV OF TECH
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Problems solved by technology
However, zein is a large protein molecule, the self-assembly process is greatly affected by operating parameters, and zein microspheres are prone to agglomeration without external force
Directly using dialysis to prepare zein microspheres has defects such as uneven particle size, uncontrollable particle size, serious agglomeration, and poor dispersion.
Method used
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Embodiment 1
[0032] Example 1-12
[0033] A preparation method of zein microspheres, comprising the following steps:
[0034] (1) if figure 1 As shown, build the dialysis device with ultrasound built-in for the preparation of zein microspheres.
[0035] (2) Select ethanol solvents with different volume fractions (V) according to Table 1, and prepare 20 mL of zein solutions with different concentrations (C).
[0036] (3) Add 100mL ultrapure water into the jacketed kettle as the dialysate, open the constant temperature tank and set the temperature (T) according to Table 1.
[0037] (4) After the temperature stabilizes, pipette 10mL of the solution obtained in step (2) and place it in a pre-treated dialysis bag with a length of 15mm, and then fix the dialysis bag with the ultrasonic horn. When fixing, the upper part can be bound with a rubber band , to retain the air in the dialysis bag and avoid being crushed when it is placed in the dialysis fluid.
[0038] (5) Put the fixed dialysis ba...
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The invention discloses a preparation method of a zein microsphere and an ultrasonic built-in dialysis device for preparing the zein microsphere. A dialysis bag of the device is positioned in a jacket kettle filled with a dialysis solution; the jacket kettle is connected with a constant-temperature tank; and an ultrasonic generator is connected with an ultrasonic transducer, the top of an ultrasonic amplitude transformer is connected with the ultrasonic transducer, the ultrasonic amplitude transformer extends into the dialysis bag and is fixed with the upper end of the dialysis bag, and the bottom of the ultrasonic amplitude transformer is positioned at the middle part of the dialysis solution in the dialysis bag. The method comprises the following steps: dissolving zein into an ethanol solution, placing a zein solution in the dialysis bag, putting the fixed dialysis bag into the dialysis solution, opening the ultrasonic generator, and controlling the ultrasonic power to be 150-225W; and performing freeze drying on a product obtained after dialysis to prepare the product. The zein microsphere obtained by using an ultrasonic built-in dialysis process disclosed by the invention is smooth in surface, has the particle size of 1-2 microns, is narrow in particle size distribution and good in dispersion performance, and can be used for oral administration and suction-type administration or can be prepared into a suspension for injection.
Description
technical field [0001] The invention relates to a zein protein, in particular to a preparation method of zein microspheres and an ultrasonic built-in dialysis device for the preparation. Background technique [0002] Microspheres are a class of new drug dosage forms with great development potential. The microsphere preparation can not only cover up the bad smell of the drug, improve the drug compliance of patients, but also regulate and control the release rate of the drug, protect the drug from being degraded by enzymes in the body, reduce the number of administration and drug stimulation, and improve the curative effect . In addition, microspheres also have a special affinity with certain cell tissues, and can be endocytosed by the reticuloendothelial system of organ tissues or fused by cells, concentrate in the target area and gradually diffuse to release drugs or be degraded by enzymes in lysosomes And release the drug, so as to play the role of targeted therapy. With...
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IPC IPC(8): A61K47/42A61K9/51
Inventor 江燕斌刘贵金魏东伟胡羽童
Owner SOUTH CHINA UNIV OF TECH