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Purification method of cleistanthus sumatranus glucoside

A technology of cycitoside and purification method, applied in the field of natural plants, can solve the problems such as the patent or literature report of cycitoside that have not been found, and achieve the effects of simplified operation, high extraction rate and reduced production cost

Inactive Publication Date: 2014-12-24
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After searching, no patents or literature reports on industrial methods suitable for mass production of cleinogenin have been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Using Cleophyllum leaves as raw materials, after crushing and passing through a 20-mesh sieve, to obtain Cleophyllia leaves coarse powder;

[0027] (2) Take 20kg of cleatinosa coarse powder and put it into the extraction device, add 80% ethanol aqueous solution 6 times the weight of the raw material, stir evenly, use ultrasonic extraction for 2 times, each time for 0.5h, and collect the extract;

[0028] (3) Add the extract to a hollow polyethersulfone ultrafiltration membrane system with a molecular weight cut-off of 6000 Daltons to remove impurities such as macromolecules to obtain a permeate;

[0029] (4) Decolorize the permeate with medicinal activated carbon, which is 0.8% of the permeate (W / V), keep it at 70°C for 30 minutes, and decolorize the decolorized solution under reduced pressure to no alcohol;

[0030] (5) Put the concentrated solution in a water bath, slowly add absolute ethanol, after it is clarified, let it stand at room temperature for 2 days, cry...

Embodiment 2

[0033] (1) Using Cleophyllum leaves as raw materials, after crushing and passing through a 20-mesh sieve, to obtain Cleophyllia leaves coarse powder;

[0034] (2) Take 20kg of cleatinosa coarse powder and put it into the extraction device, add 80% ethanol aqueous solution 12 times the weight of the raw material, stir evenly, use ultrasonic extraction twice, and collect the extract for 1 hour each time;

[0035] (3) Add the extract to a hollow polyethersulfone ultrafiltration membrane system with a molecular weight cut-off of 2000 Daltons to remove impurities such as macromolecules to obtain a permeate;

[0036] (4) Decolorize the permeate with medicinal activated carbon, which is 0.5% of the permeate (W / V), keep it at 60°C for 30 minutes, and decolorize the decolorized solution under reduced pressure to no alcohol;

[0037] (5) Put the concentrated solution in a water bath, slowly add absolute ethanol, after it is clarified, let it stand at room temperature for 1 day, naturall...

Embodiment 3

[0040] (1) Using Cleophyllum leaves as raw materials, after crushing and passing through a 20-mesh sieve, to obtain Cleophyllia leaves coarse powder;

[0041] (2) Take 20kg of cleatinosa coarse powder and put it into the extraction device, add 90% ethanol aqueous solution 10 times the weight of the raw material, stir evenly, use ultrasonic extraction twice, and collect the extract for 1 hour each time;

[0042] (3) Add the extract to a hollow polyethersulfone ultrafiltration membrane system with a molecular weight cut-off of 5,000 Daltons to remove impurities such as macromolecules to obtain a permeate;

[0043] (4) Add medicinal activated carbon to the permeate to decolorize, the medicinal activated carbon is 0.6% of the permeate (W / V), heat it at 70°C for 30 minutes, and decolorize the decolorized solution under reduced pressure to no alcohol;

[0044] (5) Put the concentrated solution in a water bath, slowly add absolute ethanol, after it is clarified, let it stand at room ...

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Abstract

The invention provides a purification method of cleistanthus sumatranus glucoside. The method is characterized by comprising the following steps of: (1) pulverizing and sieving cleistanthus sumatranus leaves used as raw materials to obtain crude powder of the cleistanthus sumatranus leaves; (2) pouring the crude powder of the cleistanthus sumatranus leaves into an extracting device for ultrasonically extracting and collecting extracting solutions; (3) adding the extracting solutions into an ultrafiltration membrane system to remove impurities such as macromolecules and the like to obtain permeating solutions; (4) decoloring the permeating solutions by adding active carbon, and performing condensation under reduced pressure on the decolored solutions until the solutions turn into alcohol-free solutions; (5) pouring concentrated solutions into water bath, slowly adding absolute ethyl alcohol into the water bath, standing and naturally crystallizing at the room temperature after the concentrated solutions are clarified, filtering to obtain a crystal substance and drying to obtain the cleistanthus sumatranus glucoside. The method is high in extraction efficiency, safe and environmentally friendly, and easy to operate and recycle solvents.

Description

technical field [0001] The invention belongs to the technical field of natural plants and relates to a method for purifying cleinogenin. Background technique [0002] Cleoside belongs to lignan glycosides, CAS number 25047-48-7, molecular formula C28H28O11, molecular weight 540.5, molecular structure formula is as follows: [0003] [0004] Cleoside has the effect of significantly increasing the number of neutrophils, can resist the reduction of neutrophils caused by drugs, can resist the reduction of white blood cells caused by benzene in rats, and can increase the number of neutrophils. [0005] After searching, no patents or literature reports on industrial methods suitable for mass production of cleinogenin have been found. Contents of the invention [0006] The object of the present invention is to provide a method for purifying cleuroside, the finished product has high cleuroside content and low production cost, and is applicable to industrial production. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH