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Method for producing saccharomycete and organic fertilizer mother solution by utilizing papermaking black liquor

A technology for papermaking black liquor and organic fertilizer is applied in the field of using papermaking black liquor to produce yeast and organic fertilizer, which can solve the problems of high cost and complicated process, and achieve the effects of low processing cost, simple process and realizing resource utilization.

Inactive Publication Date: 2014-12-24
JIAHE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the analysis of the components of pulping black liquor, it is found that the black liquor contains a large amount of organic matter such as fulvic acid, hemicellulose and cellulose decomposition products, which makes the COD of the wastewater as high as 100g / L, and the BOD is also 50g / L. Chemical methods are used for processing, the process is complicated, and a large amount of chemical reagents and equipment are required, and the cost is too high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of method utilizing papermaking black liquor to produce yeast and organic fertilizer, it mainly comprises following processing steps:

[0023] (1) Pretreatment of papermaking black liquor: filter the black liquor produced by alkaline pulping and papermaking to remove insoluble substances, gas blast for 30 minutes, add dilute acid to adjust the pH of the black liquor to 5, and then dilute it by 0.1 times;

[0024] (2) Cultivation of Candida ruthenicum strains: in a culture environment containing glucose 19 g / L, yeast extract 9 g / L, K 2 HPO 4 1.5 g / L, MgSO 4 1.2g / L culture medium, pH value 5, fermentation temperature 25°C, and culture at 180rpm for 24h;

[0025] (3) Secondary expansion culture of bacteria: Take the black liquor obtained in step (1) and dilute it by 0.1 to 10 times, add 1% urea as a nitrogen source, and add the bacteria obtained in step (2) in a proportion of 0.1% to 10% , cultured at pH 5, fermentation temperature 25°C, rotation speed 180rpm f...

Embodiment 2

[0031] A kind of concrete step that utilizes papermaking black liquor to produce yeast and organic fertilizer is:

[0032] (1) Pretreatment of papermaking black liquor: filter the black liquor obtained from sulfite pulping to remove incompatible substances, gas explosion treatment for 120 minutes, adjust the pH of the black liquor to 7 with dilute potassium hydroxide, and then dilute it by 5 times;

[0033] (2) Cultivation of Candida ruthene strains: in a medium containing glucose 19 g / L, yeast extract 9 g / L, K2HPO4 1.5 g / L, MgSO4 1.2 g / L, at pH 7, fermentation temperature Cultivate for 16 hours at 20°C with a rotational speed of 180rpm;

[0034] (3) Two-stage expanded culture of strains: take the black liquor obtained in step (1) and dilute it 5 times, add 5% urea as a nitrogen source, add the strains obtained in step (2) in a proportion of 10%, and the pH value is 7 , cultivated for 36 hours at a fermentation temperature of 20°C and a rotational speed of 180rpm;

[0035] (...

Embodiment 3

[0050] A kind of concrete step that utilizes papermaking black liquor to produce yeast and organic fertilizer is:

[0051] (1) Papermaking black liquor pretreatment: filter the black liquor obtained from sulfite pulping to remove incompatible substances, gas blast for 120 minutes, adjust the pH of the black liquor to 4 with dilute potassium hydroxide, and then dilute 3 times;

[0052] (2) Cultivation of Candida ruthene strains: in a culture medium containing glucose 19 g / L, yeast extract 9 g / L, K2HPO4 1.5 g / L, MgSO4 1.2 g / L, at pH 4, fermentation temperature Cultivate for 8 hours at 30°C with a rotational speed of 180rpm;

[0053] (3) Two-stage expanded culture of bacteria: take the black liquor obtained in step (1) and dilute it 3 times, add 3% urea as a nitrogen source, add the bacteria obtained in step (2) in a proportion of 1%, and the pH value is 5 , cultivated for 36 hours at a fermentation temperature of 30°C and a rotational speed of 180rpm;

[0054] (4) black liquor...

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Abstract

The invention discloses a method for producing a saccharomycete and an organic fertilizer mother solution by utilizing papermaking black liquor. The method is characterized by comprising the following steps of carrying out aeration pretreatment on papermaking digesting black liquor, and then regulating a pH value to 4-7; culturing candida utilis to obtain fermenting strain liquid; carrying out second-stage strain culture on the saccharomycete by utilizing diluted digesting black liquor and nutrient substances; adding a second-stage seed to the pretreated papermaking black liquor for fermentation; and after the fermentation is completed, filtering to obtain the saccharomycete; filtering a supernatant, and carrying out spraying granulation or concentration compounding to obtain an organic fertilizer. The method disclosed by the invention extends the treatment modes of the papermaking black liquor, effectively reduces the BOD (Biochemical Oxygen Demand) of black liquor, effectively realizes the recycling of the papermaking black liquor, reduces the sewage treatment cost and is simple in process and high in practicability. The saccharomycete can be used for producing ribonucleic acid and single-cell protein, and the organic fertilizer can increase the crop yield and enhance the stress resistance of crops because of rich fulvic acid and is an environmental-friendly fertilizer suitable for modern agriculture.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a method for producing yeast and organic fertilizer by using papermaking black liquor. Background technique [0002] The discharge of papermaking and paper product industrial wastewater at or above the county level in my country accounts for 18.6% of the total industrial discharge in the country, of which the amount of treatment discharge up to the standard only accounts for 49.3% of the total discharge of papermaking industrial wastewater. The pollutants contained in papermaking black liquor account for more than 90% of the total pollution discharge. Therefore, the discharge of papermaking black liquor is the main source of pollution in paper mills, and it is urgent to develop methods to deal with papermaking black liquor to avoid its pollution to the environment. In the analysis of the components of pulping black liquor, it is found that the black liquor contains a lar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C05F7/00C12R1/72
CPCY02A40/20
Inventor 董桂秀梁爱新张召佐宫玉胜杨海征徐广美
Owner JIAHE BIOTECH
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