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Method for immediately propagating pholidota cantonensis by using pholidota cantonensis rhizomes as explants

A technology of explants and rhizomes, applied in the field of plant tissue culture, can solve the problems of low natural reproduction rate, harsh growth environment requirements, slow growth, etc., and achieve the goal of increasing the reproduction coefficient, improving the survival rate of cultivation, and enhancing the growth vigor Effect

Active Publication Date: 2015-01-07
福建省农业科学院农业生物资源研究所
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] With the in-depth clinical research on Shixiantao and the development of new functions, people's demand is increasing day by day. However, due to its slow growth, small biomass, low natural reproduction rate, and harsh requirements on the growth environment, the natural resource reserves and can be developed and utilized. The quantity is very limited, and the long-term predatory mining of wild resources has led to a sharp decline in wild resources and a gradual shortage. In many places of origin, Shixiantao resources are showing a scarcity trend, so artificial cultivation is imminent.
And sufficient seedling supply is the first bottleneck to realize the artificial cultivation of Shixian peach, and the method efficiency about the propagation of Shixian peach in the prior art is extremely low, for this reason the present invention has carried out research to the propagation method of Shixian peach

Method used

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  • Method for immediately propagating pholidota cantonensis by using pholidota cantonensis rhizomes as explants
  • Method for immediately propagating pholidota cantonensis by using pholidota cantonensis rhizomes as explants
  • Method for immediately propagating pholidota cantonensis by using pholidota cantonensis rhizomes as explants

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Experimental program
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Embodiment 1

[0075] Embodiment 1 (best embodiment)

[0076] A kind of method that takes Shixian peach rhizome bud as explant rapid propagation Shixian peach, described method comprises the following steps:

[0077] (1) Preparation stage of explants

[0078] Collect wild Shixian peaches from November to April of the next year, remove the pseudobulbs, cut off the terminal buds with rhizomes on the rhizomes as explants, remove the old scales and roots covered on the rhizomes, spare;

[0079] (2) Aseptic treatment stage, including the following steps in sequence:

[0080] 1. After the explants prepared in step (1) are rinsed with clear water earlier, remove redundant rhizomes, so that only the rhizomes below 2 mm are reserved on each bud;

[0081] ② Then transfer to the sterilized ultra-clean workbench, rinse with sterile water for 3 times, and then transfer to a sterile bottle;

[0082] ③Then sterilize with 75% alcohol by volume for 3 minutes, then rinse with sterile water for 3 times;

...

Embodiment 2

[0095] A kind of method that takes Shixian peach rhizome bud as explant rapid propagation Shixian peach, described method comprises the following steps:

[0096] (1) Preparation stage of explants

[0097] Collect wild Shixian peaches from November to April of the next year, remove the pseudobulbs, cut off the terminal buds with rhizomes on the rhizomes as explants, remove the old scales and roots covered on the rhizomes, spare;

[0098] (2) Aseptic treatment stage, including the following steps in sequence:

[0099] 1. After the explants prepared in step (1) are rinsed with clear water earlier, remove redundant rhizomes, so that only the rhizomes below 2 mm are reserved on each bud;

[0100] ② Then transfer to the sterilized ultra-clean workbench, rinse with sterile water for 5 times, and then transfer to a sterile bottle;

[0101] ③Then sterilize with 70% alcohol by volume for 3 minutes, then rinse with sterile water for 5 times;

[0102] ④ Afterwards, pour mercury chlori...

Embodiment 3

[0114] A kind of method that takes Shixian peach rhizome bud as explant rapid propagation Shixian peach, described method comprises the following steps:

[0115] (1) Preparation stage of explants

[0116] Collect wild Shixian peaches from November to April of the next year, remove the pseudobulbs, cut off the terminal buds with rhizomes on the rhizomes as explants, remove the old scales and roots covered on the rhizomes, spare;

[0117] (2) Aseptic treatment stage, including the following steps in sequence:

[0118] 1. After the explants prepared in step (1) are rinsed with clear water earlier, remove redundant rhizomes, so that only the rhizomes below 2 mm are reserved on each bud;

[0119] ② Then transfer to the sterilized ultra-clean workbench, rinse with sterile water for 4 times, and then transfer to a sterile bottle;

[0120] ③Then sterilize with 70% alcohol by volume for 3 minutes, then rinse with sterile water 4 times;

[0121] ④ Afterwards, pour mercury chloride w...

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Abstract

The invention relates to a method for propagating pholidota cantonensis, and particularly relates to a method for immediately propagating pholidota cantonensis by using pholidota cantonensis rhizomes as explants, belonging to the technical field of plant tissue culture. The method for immediately propagating the pholidota cantonensis by using pholidota cantonensis rhizomes as the explants comprises the following steps sequentially: (1) a preparing stage of the explants, namely collecting the pholidota cantonensis in a period from November every year to April in next year, removing pseudobulbs, cutting terminal buds or axillary buds with the rhizomes on the rhizomes off, and removing old scales and roots covering the rhizomes for later use; (2) an asepsis stage; (3) a primary culture stage; (4) a subculture strong seedling culturing stage; and (5) a rooting culturing stage. The method provided by the invention is capable of effectively improving the reproduction efficiency of the pholidota cantonensis so that sufficient pholidota cantonensis regenerated plantlets are obtained, and lays the foundation for the large-scale production and application.

Description

technical field [0001] The invention relates to a method for propagating shixiantao, in particular to a method for rapidly propagating shixiantao with rhizomes of shixiantao as explants, and belongs to the technical field of plant tissue culture. Background technique [0002] Pholdota chinensis Lindl. is a perennial epiphytic herb of the Orchidaceae genus Pholidota, commonly known as Pholdota chinensis Lindl. The height of the plant is 15-25cm, the rhizome is thick, horizontal, with many, several fibrous roots; the pseudobulb is ovate-oblong or oblong, 2-7cm long, 0.5-2.5cm thick, with 2 leaves at the top . Leaves obovate-lanceolate, obovate or obovate-elliptic, 8-18cm long, 2-5cm wide, apex acute or acuminate, basically extending downward into long stalks, usually with obvious arc-shaped veins 3 -5 bars. Scape longer than the top of the pseudobulb, erect or more or less drooping, with multiple scales at the base; inflorescence terminal, with several to 20 flowers; bracts...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈菁瑛刘保财黄颖桢赵云青
Owner 福建省农业科学院农业生物资源研究所
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