A time-point quantitative processing method for the production of full triploid of Hong Kong oyster

A Hong Kong oyster and triploid technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as low induction rate and unstable doubling rate

Active Publication Date: 2016-01-20
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defects of low induction rate and unstable doubling rate in the traditional triploid production method, thereby proposing a time point quantitative processing method using the developmental stage of fertilized eggs as a biological index , can produce 100% Hong Kong oyster triploid

Method used

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  • A time-point quantitative processing method for the production of full triploid of Hong Kong oyster

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] a. Acquisition of single eggs: In late June 2013, 30 mature Hong Kong oyster females aged 3-5 were selected from local Hong Kong oysters at the Zhanjiang Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences; the number and quality of eggs Selected with double indicators, and selected 6 individuals with neat development, an average number of eggs of 80 million, complete egg membranes, and dark yellow yolk accumulation as female parents; collected individual eggs, soaked in seawater with a salinity of 18ppt for 45 minutes , when the germinal vesicles are 100% completely ruptured, eggs are obtained. The eggs were washed three times with fresh seawater with a salinity of 18ppt to obtain single egg liquid, and the eggs were all round or oval.

[0019] b. Obtain single sperm: use the core population in step a as the material, screen out 12 male individuals of 2-3 years old; then test the quality of their sperm, and screen out indivi...

Embodiment 2

[0023] a. Acquisition of single eggs: In late May 2014, at the Zhanjiang Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, 25 mature Hong Kong oyster females of 3-5 years old were selected from the Zhuhai population of Hong Kong oysters; Selected with dual indicators of quality, and selected 5 individuals with neat development, an average number of eggs of 120 million, complete egg membranes, and dark yellow yolk accumulation as female parents; collected individual eggs, and placed them in seawater with a salinity of 20ppt Soak for 30 minutes, until 100% of the germinal vesicles are completely ruptured to obtain ovum. The ovum is washed repeatedly 3 times with fresh seawater with a salinity of 20ppt to obtain single ovum liquid, and the ovum is all round or oval.

[0024] b. Obtain single sperm: use the core population in step a as the material, screen out several 10 male individuals of 2-3 years old; then test the quality of the...

Embodiment 3

[0028] a. Acquisition of single eggs: In early May 2014, 27 mature 3-5-year-old Hong Kong oyster females were selected at the Guangxi Shellfish Comprehensive Experimental Station using Hong Kong oysters from the Maowei Sea population as materials; Selected with dual quality indicators, and selected 9 individuals with neat development, an average number of eggs of 60 million, complete egg membranes, and dark yellow yolk accumulation as female parents; collect individual eggs and soak them in seawater with a salinity of 16ppt After 40 minutes, 100% of the germinal vesicles were completely ruptured to obtain eggs. The eggs were washed three times with fresh seawater with a salinity of 16ppt, and the eggs were all round or oval.

[0029] b. Obtaining single sperm: using the core population in step a as the material, select 15 male individuals of 2-3 years of age; then test the quality of their sperm, and screen out individuals with ≥30% sperm activity under the microscope , activ...

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Abstract

The invention discloses a time point quantification treatment method for producing a Crassostrea hongkongensis all-triploid. The time point quantification treatment method in which a fertilized egg development stage is taken as a biological indicator is created through the technical links of collection of single fertilized eggs, timely treatment of the fertilized eggs, detection of larva ploidy and the like, so that a 100-percent Crassostrea hongkongensis all-triploid can be obtained. Through optimization and pairing of the single fertilized eggs, the defect of poor mixed fertilized egg synchronicity in a conventional Crassostrea hongkongensis triploid induction process is overcome effectively, and zygotes with high synchronicity are obtained. A conventional fixed thinking mode in which a time point when 30-50 percent of a first polar body occurs is taken as a treatment time point and the optimal time interval is 20 minutes is overturned. Instead, the fertilized egg development stage is taken as the biological indicator, and A+1 / 3B and B+C are taken as a treatment time point and a treatment time interval respectively, so that the influences of external environmental conditions such as temperature on treatment are avoided, and the Crassostrea hongkongensis all-triploid can be induced successfully.

Description

Technical field: [0001] The invention belongs to the technical field of shellfish genetics and breeding in marine agriculture, and in particular relates to a time-point quantitative processing method for producing Hong Kong oyster whole triploid. Background technique: [0002] my country is a big oyster farming country, with an output of about 3.5-3.8 million tons from 2009 to 2013; while the output of Hong Kong oyster (Crassostrea hongkongensis) is about 1.3 million tons, which accounts for 30% of oyster production and 12% of cultured shellfish production. It accounts for 7% of the total production of mariculture and more than 21% of the world production (China Fisheries Yearbook, 2014). Hong Kong oysters are distributed in Guangdong, Guangxi, and Fujian in my country. They are shellfish with high economic value that grow in warm-temperate coastal areas. They like high-temperature and low-salt environments. Amino acids, known as "milk in the ocean", are an important marine b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张跃环喻子牛苏家齐张扬李军李琼珍
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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