Drought resistant correlative protein of plant and coding gene and application thereof

一种基因、植物的技术，应用在植物抗旱相关蛋白及其编码基因与应用领域，能够解决影响农业生产、植物死亡等问题

Active Publication Date: 2015-01-21
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Plants grow and develop in a complex and changeable environment, and are often subject to adversity stress, among which drought is the main adversity factor that affects and limits plant growth and development, and can even lead to plant death, seriously affecting agricultural production

Method used

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  • Drought resistant correlative protein of plant and coding gene and application thereof
  • Drought resistant correlative protein of plant and coding gene and application thereof
  • Drought resistant correlative protein of plant and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1, the acquisition of protein ZmDREB2.7 and its coding gene

[0043] Take the seeds of corn inbred line B73, and after accelerating germination at 28°C for three days, transfer the germinated seeds to nutrient soil or nutrient solution for three weeks, and take the whole plant to freeze and grind in liquid nitrogen, extract total RNA, and perform reverse transcription , to obtain cDNA, using the cDNA as a template, 5'-ATGGATCGGGTGCCGCCG-3' and 5'-TCAAAGAGGGACGACGAGC-3' as primers for PCR amplification, amplified products were subjected to agarose gel electrophoresis, and a 1.1kb DNA fragment was isolated and purified Sequencing was carried out, and the results showed that the sequence of the DNA fragment is shown in the 99th-1178th position of sequence 2 of the sequence listing.

[0044] Sequence Listing Sequence 2 is the full-length cDNA sequence of the protein ZmDREB2.7 shown in Sequence Listing Sequence 1 in maize inbred line B73, in which the 1st-98th positi...

Embodiment 2

[0045] Example 2. Overexpression of gene ZmDREB2.7 improves drought resistance of Arabidopsis

[0046] 1. Construction of recombinant vector

[0047] The DNA fragment shown in the 99th-1178th position of Sequence Listing Sequence 2 was cloned into the vector pGKX between the Sma I and Sal I restriction sites (located downstream of the 35S promoter), and confirmed by sequencing to obtain the recombinant vector pGZ.

[0048] 2. Acquisition of recombinant Agrobacterium tumefaciens

[0049] Transform the recombinant vector pGZ into the Agrobacterium tumefaciens GV3101+pSoup strain to obtain recombinant Agrobacterium X containing the recombinant vector pGZ;

[0050] The vector pBI121 was transformed into the Agrobacterium tumefaciens GV3101 strain to obtain the recombinant Agrobacterium CK containing the vector pBI121.

[0051] 3. Obtaining transgenic Arabidopsis

[0052] The recombinant Agrobacterium X was transformed into wild-type Colombian ecotype Arabidopsis thaliana by flo...

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Abstract

The present invention provides a method of producing transgenic plants which are drought resistant, plants obtainable by the method and uses thereof.

Description

technical field [0001] The invention relates to a plant drought resistance related protein ZmDREB2.7 and its coding gene and application. Background technique [0002] Plants grow and develop in a complex and changeable environment, and are often subjected to adversity stress, among which drought is the main stress factor that affects and limits plant growth and development, and even causes plant death, seriously affecting agricultural production. Therefore, cultivating drought-resistant crop varieties has always been one of the main goals of agricultural science and technology research. Maize (Zea mays L.) is one of the most important food crops. Drought-resistant gene mining in maize is of great significance for breeding drought-resistant maize varieties and improving maize yield. Contents of the invention [0003] The object of the present invention is to provide a plant drought resistance related protein ZmDREB2.7 and its coding gene and application. [0004] The pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10C12N1/15C12N1/19C12N1/21C12N7/01A01H5/00
CPCC12N15/8273C07K14/415C12N15/8237
Inventor 秦峰刘升学王向兰王宏伟
Owner INST OF BOTANY CHINESE ACAD OF SCI
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