Phosphatidase A1 immobilization method

A technology of phospholipase and immobilized phospholipase, which is used in the food and biological fields, and can solve problems such as difficult large-scale application, narrow application range, and high price

Inactive Publication Date: 2015-01-21
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] But above-mentioned existing about the immobilization method of phospholipase A1, all there is certain defective in application, especially under the situation of producing diacylglycerol, the application range of immobilized phospholipase is narrow, and effect is not outstanding, and in industry It is difficult to implement large-scale applications on the
In addition, when it is applied to the production of DHA phospholipids, the reaction time is too long, which will easily cause the oxidation of DHA
Moreover, in the existing literature and patents, it is rarely mentioned that immobilized phospholipase A1 can be used for esterification reaction. Regarding the esterification of immobilized enzymes, only Novozyme 435 produced by Novozymes has a strong esterification function on the market. , but expensive

Method used

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  • Phosphatidase A1 immobilization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Rinse the D213 carrier with distilled water to remove impurities, soak in 0.5mol / L NaOH solution for 2h, wash with water until neutral, then soak in 0.5mol / L HCl solution for 2h, wash with water until neutral, repeat the above steps 3 times , and finally soaked in 0.5mol / L NaOH solution for 2h, washed with water until neutral.

[0085] Weigh 40g of the processed carrier D21340g into a 250ml conical flask, add 80ml of lecitase Ultra enzyme solution, place it in a shaker at 25°C at 150rpm, shake it at 150rpm, take out the mixture after 1.5h for suction filtration, and fluidize the solid part to a fluidized bed (Midi Glatt) The air inlet temperature is 40°C, the air inlet pressure is 0.6 bar, and dried to a moisture content of about 5%. Labeled Enzyme 1.

Embodiment 2

[0087] Weigh 140 g of the carrier D31 treated according to the carrier treatment method in Example 1 into a 250 ml Erlenmeyer flask, add 80 ml of lecitase Ultra enzyme solution, place it in a shaker at 25°C at 150 rpm, and take out the mixture after 1.5 hours for suction filtration, and remove the solid part Fluidized drying, fluidized bed (Midi Glatt) inlet air temperature 40 ℃, inlet air pressure 0.6 bar, dry to about 5% moisture content. Labeled Enzyme 2.

Embodiment 3

[0089] Weigh 140 g of the carrier D20 treated according to the carrier treatment method in Example 1 into a 250 ml Erlenmeyer flask, add 80 ml of lecitase Ultra enzyme solution, place it in a shaker at 25°C at 150 rpm, and take out the mixture after 1.5 hours for suction filtration. Fluidized drying, fluidized bed (Midi Glatt) inlet air temperature 40 ℃, inlet air pressure 0.6 bar, dry to about 5% moisture content. Labeled Enzyme 3.

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Abstract

The invention relates to a phosphatidase A1 immobilization method. The method comprises the following steps: 1, allowing a carrier selected from macroporous adsorption resin or ion exchange resin to contact with phospholipase A1 in order to immobilize the phospholipase A1 on the carrier; and 2, drying the phospholipase A1 immobilized carrier obtained in step 1 in order to obtain immobilized phospholipase A1. The invention also provides immobilized phospholipase A1 prepared through the method, and an application of the immobilized phospholipase A1. The immobilized phospholipase A1 has the functions of esterification and acid hydrolysis, can be repeatedly used in the oil industry, and has the advantages of high reaction efficiency low cost and potential industrial application.

Description

technical field [0001] The invention relates to the fields of food and biotechnology. Specifically, the present invention relates to a method for immobilizing phospholipase A1. Background technique [0002] Phospholipase A1 is a carboxylate hydrolase with both lipase and phospholipase activities. Compared with lipase, this enzyme has high hydrolysis activity, low price and broad application prospects. [0003] Enzyme immobilization technology refers to the use of certain technical means to bind enzymes in a certain area so that they can perform their unique catalytic reactions and can be recycled and reused. Through this technology, the efficiency of enzyme use can be improved, the immobilized enzyme can be separated from the product and reused, the stability of the enzyme is improved, and it is convenient for continuous and automatic operation. [0004] The immobilized enzyme technology that can be used for fat modification, transesterification, esterification etc. descri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08
Inventor 郑妍辛本荣杨武林杨天奎徐学兵
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
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