Phosphatidase A1 immobilization method

A technology of phospholipase and immobilized phospholipase, which is used in the food and biological fields, and can solve problems such as difficult large-scale application, narrow application range, and high price
CN104293764AInactive Publication Date: 2015-01-21WILMAR SHANGHAI BIOTECH RES & DEV CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WILMAR SHANGHAI BIOTECH RES & DEV CENT
Publication Date
2015-01-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a phosphatidase A1 immobilization method. The method comprises the following steps: 1, allowing a carrier selected from macroporous adsorption resin or ion exchange resin to contact with phospholipase A1 in order to immobilize the phospholipase A1 on the carrier; and 2, drying the phospholipase A1 immobilized carrier obtained in step 1 in order to obtain immobilized phospholipase A1. The invention also provides immobilized phospholipase A1 prepared through the method, and an application of the immobilized phospholipase A1. The immobilized phospholipase A1 has the functions of esterification and acid hydrolysis, can be repeatedly used in the oil industry, and has the advantages of high reaction efficiency low cost and potential industrial application.
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Description

technical field

[0001] The invention relates to the fields of food and biotechnology. Specifically, the present invention relates to a method for immobilizing phospholipase A1. Background technique

[0002] Phospholipase A1 is a carboxylate hydrolase with both lipase and phospholipase activities. Compared with lipase, this enzyme has high hydrolysis activity, low price and broad application prospects.

[0003] Enzyme immobilization technology refers to the use of certain technical means to bind enzymes in a certain area so that they can perform their unique catalytic reactions and can be recycled and reused. Through this technology, the efficiency of enzyme use can be improved, the immobilized enzyme can be separated from the product and reused, the stability of the enzyme is improved, and it is convenient for continuous and automatic operation.

[0004] The immobilized enzyme technology that can be used for fat modification, transesterification, esterification etc. descri...

Claims

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