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Functional SSR labels obviously related with wood quality characters in populus CesAs gene, and applications and kit thereof

A technology of quality traits and poplars, applied in the fields of application, plant gene improvement, DNA/RNA fragments, etc., can solve the problem of not too close linkage of phenotypic traits of species, achieve the effect of shortening the breeding cycle of forest trees and improving the development

Inactive Publication Date: 2015-01-21
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

Most of the previously developed SSR loci come from random regions of the genome, and these SSRs are mostly neutrally evolved, and are not closely linked to the formation of phenotypic traits in species

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  • Functional SSR labels obviously related with wood quality characters in populus CesAs gene, and applications and kit thereof
  • Functional SSR labels obviously related with wood quality characters in populus CesAs gene, and applications and kit thereof
  • Functional SSR labels obviously related with wood quality characters in populus CesAs gene, and applications and kit thereof

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Embodiment Construction

[0023] It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0024] In view of the characteristics of high heterozygosity, long cycle, and outcrossing system of trees, some methods developed in crop genetics research are difficult to implement in forest trees. Therefore, the current major progress in molecular genetics of forest trees is through molecular marker-assisted selection strategies. By dissecting multiple genes that affect wood fiber traits, we can study these quantitative trait loci just like studying quality trait loci, study the positions of these genes on chromosomes, and look for molecular markers that are closely linked to them to genetically improve trees. Its main application methods are quantitative trait mapping based on family popul...

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Abstract

Functional SSR labels obviously related with wood quality characters in a populus CesAs gene, and applications and a kit thereof are disclosed. The functional SSR labels comprise 7A-SSR2 and 7B-SSR1. The 7A-SSR2 is in the exon 3 zone of populus CesA7-A and is (TGA)n. The 7B-SSR1 is in the intron 4 zone of populus CesA7-B and is (TTAA)m. The determined functional SSR labels provide new technical means for early-period screening of breeds with high-quality and for shortening the breeding cycle of forest trees, provide genetic evidences for molecular research for improvement of wood quality characters, and have important significance for improving development of forest genetic breeding engineering in China and for forest breeding strategy implementation at present.

Description

technical field [0001] The invention relates to the field of plant molecular breeding, in particular to a functional SSR marker in the CesAs gene of poplar which is significantly associated with wood quality traits, its application and a kit. Background technique [0002] Poplar grows rapidly, has a short rotation period, is relatively easy for interspecific hybridization and asexual reproduction, and is convenient for genetic manipulation. It is considered to be the most promising fiber energy tree species in the northern hemisphere (Li et al., 2008, The Plant Journal, 54: 569-581). Especially with the rapid development of our country's economy, the current forest production and quality are far from meeting the needs of industry. Therefore, breeding new germplasm of high-quality timber, developing plantation forests of high-quality industrial timber, and increasing the yield per unit area of ​​forest land are the solutions to this problem. Important coping strategies. Howe...

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68A01H1/04
Inventor 张德强杜庆章徐煲铧
Owner BEIJING FORESTRY UNIVERSITY
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