NlAKTIP gene related to growth and development of brown planthopper as well as encoding protein and application thereof

Abstract

The invention discloses an NlAKTIP gene related to growth and development of brown planthopper as well as an encoding protein and application thereof. The AKT interacting protein encoding gene NlAKTIP for regulating and controlling the growth and development of the brown planthopper comprises a nucleotide sequence as shown in SEQ ID NO: 1 and homologous mutant sequences of the nucleotide sequence, the gene plays an important role in maintaining normal growth and development processes of the brown planthopper, the function of the gene is inhibited so as to result in the slow growth of the brown planthopper, the brown planthopper becomes smaller and the eclosion time of the brown planthopper imago is delayed. The protein encoded by the brown planthopper NlAKTIP gene comprises a nucleotide sequence as shown in SEQ ID NO: 2 and homologous mutant sequences of the nucleotide sequence, the function of the gene is inhibited so as to result in the slow growth of the brown planthopper, the brown planthopper becomes smaller and the eclosion time of the brown planthopper imago is delayed. The application of the RNA interference of the brown planthopper NlAKTIP gene in control of the brown planthopper is also disclosed. The RNA interference is successfully implemented to the gene and the results show that the growth and development of the brown planthopper is obviously inhibited, the size is decreased, the weight is reduced and the eclosion time is delayed.

Description

technical field [0001] The invention relates to a gene related to the growth and development of brown planthopper, encoded protein and application thereof. Background technique [0002] Brown planthopper (Nilaparvata ) is a long-distance migratory rice pest, which mainly damages rice plants by sucking phloem sap and spreading rice viruses. In recent years, the brown planthopper has developed into an important pest in Asian rice regions, with an annual damage area of ​​more than 20 million hm2, causing billions of kilograms of rice yield loss (Lin Yongjun et al., 2011). At present, the main emergency control measures against brown planthoppers are spraying chemical pesticides. The extensive abuse of chemical pesticides not only seriously pollutes the environment, but also induces the resistance of brown planthoppers to the long-term application of chemical pesticides (Wang Peng et al., 2013). Among the pesticides used to control brown planthoppers, the effective pesticide...

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Problems solved by technology

The massive abuse of chemical pesticides not only seriously pollutes the environment, but also induces resistance in brown planthoppers (Wang Peng et al., 2013)

Among the pesticides used to control brown planthoppers, the effective pesticides that have played a good role in the past, such as fipronil, imidacloprid, buprofezin, etc., have been eliminated or restricted in use. When carrying out prevention and control, I feel helpless in the choice of pesticides, and chemical control is facing an embarrassing situation that cannot be prevented

The reason is that most of these chemical pesticides aim at large-scale and high-intensity killing of pests. However, due to practical reasons such as the extremely complex genetic diversity of the brown planthopper population, some of them can survive. As a result of high selection pressure, eventually lead to the formation of more adaptive populations

On the other hand, while chemical pesticides kill pests, they will also endanger non-target organisms including natural enemies, which will inevitably have a negative impact on the farmland ecosystem, and the ecological control function has not waited for its due performance

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