Fingerprint chromatogram identification method of amomum tsaoko and alpinia katsumadai

Abstract

The invention provides a fingerprint chromatogram identification method for rapidly identifying amomum tsaoko and alpinia katsumadai. The method comprises the following steps: preparing a test solution via ultrasound-assisted extraction or cold-maceration extraction by taking ethyl acetate, petroleum ether or dichloromethane as an extracting solvent; and carrying out measurement to obtain a fingerprint chromatogram under a certain condition via a GC-MS method so as to identify amomum tsaoko and alpinia katsumadai. Scientific experiments prove that the method is simple, convenient and rapid to operate, and the measured fingerprint chromatogram is high in repeatability and strong in characteristic property, so that the method can be applied to the rapid identification and the quality control on amomum tsaoko and alpinia katsumadai.

Description

technical field [0001] The invention relates to the field of identification of traditional Chinese medicines, in particular to a fingerprint identification method of herbs and herbal medicines. Background technique [0002] Caoguo, also known as Caoguozi, was first recorded in "Compendium of Materia Medica". The 2010 edition of "Chinese Pharmacopoeia" recorded Caoguo as the dry and mature fruit of Amomum tsao-ko Crevost et Lemaire, a plant of the ginger family. , the effect of removing phlegm and cutting malaria; and cardamom is the dried and nearly mature seeds of the ginger plant Alpinia Katsumadai Hayata, which has the effect of drying dampness and promoting qi, warming and relieving vomiting; grass fruit and cardamom have different effects and clinical applications. However, because the two are both ginger medicinal materials, their appearance and smell are very similar, and they are often confused for use. In order to differentiate and identify the grass fruit and the ...

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Problems solved by technology

Recently, there have been reports of using steam distillation or supercritical fluid to extract tsaoguo or cardamom volatile oil, and then use GC-MS method to determine the fingerprint of its volatile oil, but because these methods usually require a large amount of medicinal materials, the extraction process is more troublesome , the extraction time is generally relatively long; and the steam distillation method may cause damage and loss of some active ingredients due to high temperature and long extraction time, so it is not suitable as a rapid identification method for medicinal materials

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