Fingerprint chromatogram identification method of amomum tsaoko and alpinia katsumadai

The technology of fingerprint spectrum and standard fingerprint spectrum is applied in the field of traditional Chinese medicine identification, which can solve the problems of rapid identification method of unsuitable medicinal materials, destruction of effective components, troublesome extraction process, etc., so as to achieve simple and fast extraction and analysis process, easy operation, and avoid high extraction temperature. Effect

Inactive Publication Date: 2015-01-21
GUANGXI UNIV OF CHINESE MEDICINE
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  • Application Information

AI Technical Summary

Problems solved by technology

Recently, there have been reports of using steam distillation or supercritical fluid to extract tsaoguo or cardamom volatile oil, and then use GC-MS method to determine the fingerprint of its volatile oil, but because these methods usually require a large amount of medicinal materials, the extraction process is more troublesome , the extraction time is generally relatively long; and the steam distillation method may cause damage and loss of some active ingredients due to high temperature and long extraction time, so it is not suitable as a rapid identification method for medicinal materials

Method used

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  • Fingerprint chromatogram identification method of amomum tsaoko and alpinia katsumadai
  • Fingerprint chromatogram identification method of amomum tsaoko and alpinia katsumadai
  • Fingerprint chromatogram identification method of amomum tsaoko and alpinia katsumadai

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Determination of the common pattern of fingerprints of Fructus Fructus and Fructus Cardamom

[0033] 1 Experimental material

[0034] 1.1 Experimental medicinal materials: The medicinal herbs of grass fruit and cardamom used in the experiment were purchased from large pharmacies in various provinces and cities across the country. The herbal medicinal materials were identified by Professor Cai Yi from the Chinese Medicine Appraisal Department of Guangxi University of Traditional Chinese Medicine as the dried and mature fruit of Amomum tsao-ko Crevost et Lemaire; Katsumadai Hayata) dried ripe fruit.

[0035] 1.2 Pre-treatment of experimental medicinal materials: Take the cayenne fruit and nutmeg nut from each origin, pulverize with a pulverizer, pass through a 40-mesh sieve, and set aside.

[0036] 1.3 Experimental reagents: ethyl acetate (analytical grade, Sinopharm Chemical Reagent Co., Ltd.), anhydrous sodium sulfate (analytical grade, Tianjin Damao Chemical ...

Embodiment 2

[0055] Example 2 Influence of different extraction solvents and extraction methods on the GC-MS fingerprint of Caoguo

[0056] 1 Experimental materials: The experimental herbs and fruits were purchased from Jiajiakang Pharmacy, Heshan District, Yiyang City, Hunan Province. They were identified as Amomum tsao-ko crevost et lemaire by Professor Cai Yi of the Chinese Medicine Identification Teaching and Research Office of Guangxi University of Traditional Chinese Medicine. ) of dry ripe fruit.

[0057] 1.2 Pretreatment of experimental medicinal materials: the same as in Example 1.

[0058] 1.3 Experimental reagents: petroleum ether (60-90° C.), dichloromethane, ethyl acetate (analytical grade, Sinopharm Chemical Reagent Co., Ltd.), anhydrous sodium sulfate (analytical grade, Tianjin Damao Chemical Reagent Factory).

[0059] 2. Experimental apparatus: the same as in Example 1.

[0060] 3. GC-MS analysis conditions: the same as in Example 1.

[0061] 4. Preparation of the test s...

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Abstract

The invention provides a fingerprint chromatogram identification method for rapidly identifying amomum tsaoko and alpinia katsumadai. The method comprises the following steps: preparing a test solution via ultrasound-assisted extraction or cold-maceration extraction by taking ethyl acetate, petroleum ether or dichloromethane as an extracting solvent; and carrying out measurement to obtain a fingerprint chromatogram under a certain condition via a GC-MS method so as to identify amomum tsaoko and alpinia katsumadai. Scientific experiments prove that the method is simple, convenient and rapid to operate, and the measured fingerprint chromatogram is high in repeatability and strong in characteristic property, so that the method can be applied to the rapid identification and the quality control on amomum tsaoko and alpinia katsumadai.

Description

technical field [0001] The invention relates to the field of identification of traditional Chinese medicines, in particular to a fingerprint identification method of herbs and herbal medicines. Background technique [0002] Caoguo, also known as Caoguozi, was first recorded in "Compendium of Materia Medica". The 2010 edition of "Chinese Pharmacopoeia" recorded Caoguo as the dry and mature fruit of Amomum tsao-ko Crevost et Lemaire, a plant of the ginger family. , the effect of removing phlegm and cutting malaria; and cardamom is the dried and nearly mature seeds of the ginger plant Alpinia Katsumadai Hayata, which has the effect of drying dampness and promoting qi, warming and relieving vomiting; grass fruit and cardamom have different effects and clinical applications. However, because the two are both ginger medicinal materials, their appearance and smell are very similar, and they are often confused for use. In order to differentiate and identify the grass fruit and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 覃洁萍何俏明李耀华梁臣艳
Owner GUANGXI UNIV OF CHINESE MEDICINE
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