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Method for promoting germination of oriental lily hybrid seed

A hybrid seed and lily technology, applied in the biological field, can solve the problems of seed mold and rot, irregular germination, cumbersome operation, etc.

Active Publication Date: 2015-01-28
北京市辐射中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The natural germination time of oriental lily hybrid seeds takes as little as 30 days, and as long as 90-110 days, which is easy to cause mold and rot, and the germination is irregular and the germination rate is extremely low, which restricts the process of lily industrialization development
The routinely used embryo rescue method mostly produces white radicles after 60 days of pollination, which is not only cumbersome to operate, but also has strict requirements on the treatment period.

Method used

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  • Method for promoting germination of oriental lily hybrid seed
  • Method for promoting germination of oriental lily hybrid seed
  • Method for promoting germination of oriental lily hybrid seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, shearing promotes the influence of oriental lily hybrid seed germination

[0035] 1. Sample preparation

[0036] In June 2012, the oriental lily cultivars 'Casablanca' and Marco Polo' were used as the female parent and 'Sorbonne' as the male parent in the Germplasm Resource Garden of Beijing Radiation Center, and the seeds of the hybrid progeny were harvested in October and kept at 4°C Refrigerate to obtain samples. The hybrid parent materials were all purchased from the Netherlands, and the seeds of the hybrid offspring were derived from conventional cross pollination.

[0037] 2. Cutting treatment

[0038] In 2013, the mature hybrid progeny seeds with full grains, clear embryos (you can see the slender part of the endosperm in the middle of the endosperm) were selected for the following three treatments:

[0039] Treatment 1 (CK): Dried seeds, sterilized and filtered, were put into a Erlenmeyer flask containing medium (MS+30g / l sucrose+9g / l agar), num...

Embodiment 2

[0070] Embodiment 2, the influence of different sucrose concentration culture media on the germination rate of oriental lily hybrid seeds

[0071] Configure three media with different sucrose concentrations, MS+20g / l sucrose+9g / l agar, MS+30g / l sucrose+9g / l agar, MS+40g / l sucrose+9g / l agar, seed treatment The method is the seed treatment method in the M4 screened in the above-mentioned embodiment 1, and the culture medium with different sucrose concentrations is inserted, as follows:

[0072] Cut off the endosperm and wings around the dry seed embryos, sterilize and filter the remaining embryos, and put them into a Erlenmeyer flask containing medium (MS+20g / l sucrose+9g / l agar), and put them into the Erlenmeyer flask , No. M5;

[0073] Cut off the endosperm and wings around the dry seed embryos, sterilize and filter the remaining embryos, put them into a triangular flask containing medium (MS+40g / l sucrose+9g / l agar), and put them in the triangular flask , No. M6.

[0074] ...

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Abstract

The invention discloses a method for promoting germination of oriental lily hybrid seed. The provided method for promoting germination of oriental lily hybrid seed comprises the following steps: 1) cutting oriental lily hybrid seed embryo to remove the endosperm at the periphery of embryo and wings, so as to obtain residual embryo; and 2) culturing the residual embryo, so as to promote germination of lily hybrid seed. The method provides technology reference for accelerating the breeding process of oriental lily, and has certain reference meaning on lily seed tissue culture and rapid propagation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for promoting germination of oriental lily hybrid seeds. Background technique [0002] Lily (Lilium spp.) is a perennial bulbous plant and is a famous ornamental flower at home and abroad. Among the four types of lilies popular in the market, oriental lilies account for the largest cultivation proportion, and belong to the group with the largest and most beautiful flowers among the lily hybrid lines. The conventional propagation methods in the production of oriental lily, such as dividing bulbs, dividing bulbils, cutting scales, embedding scales, etc., have a small number of reproduction and may easily cause seedlings to degenerate. Lily has a long growth cycle, and it takes 2-3 years from seed to flowering. Tissue culture not only shortens the growth cycle of lily, greatly improves the reproduction coefficient, but also solves the problems of seedling degradation and many...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01H4/00C12N5/04
Inventor 白锦荣郎利新孔滢窦晓莹包放尚宏忠霍晨思
Owner 北京市辐射中心