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Bacillus sp., and method for producing flocculating agent through composite wastewater cultivation of Bacillus sp.

A technology of Bacillus and Bacillus, which is applied in the field of flocculant production by co-cultivating Bacillus in monosodium glutamate wastewater and soybean product wastewater, can solve the problems of high preparation cost, low flocculation efficiency, unclear metabolic pathway and regulation mechanism, etc., and achieve improved dehydration performance effect

Inactive Publication Date: 2015-01-28
WENZHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] At present, there are still some problems in the research of biological flocculants, mainly as follows: (1) low flocculation efficiency and high preparation cost; (2) metabolic pathways, especially the metabolic pathways and regulation mechanisms of complex flocculants produced by various strains are still unclear (3) Large-scale fermentation technology research from the perspective of large-scale industrial production and key issues urgently needed to be solved (such as reducing costs, improving floc production capacity, production regulation, and improving floc production stability, etc.) is almost blank

Method used

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  • Bacillus sp., and method for producing flocculating agent through composite wastewater cultivation of Bacillus sp.
  • Bacillus sp., and method for producing flocculating agent through composite wastewater cultivation of Bacillus sp.
  • Bacillus sp., and method for producing flocculating agent through composite wastewater cultivation of Bacillus sp.

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preparation example Construction

[0086]The preparation method of flocculant of the present invention, described method comprises the following steps:

[0087] (a) Inoculate the bacillus with the preservation number CGMCC NO.9143 in the LB medium, and cultivate it for 18-24 hours to obtain the primary seed liquid;

[0088] (b) cultivating the primary seed liquid obtained in step (a) for 18-24h in the seed medium to obtain the secondary seed liquid;

[0089] (c) inoculating the secondary seed solution obtained in step (b) into a fermentation medium and culturing to obtain the flocculant.

[0090] In another preferred example, the flocculant is polysaccharide.

[0091] In another preferred embodiment, the step (a) is cultivated under the conditions of 35-45° C. and 150-180 rpm.

[0092] In another preferred embodiment, the step (b) is cultivated under the condition of 35-45°C, and the step (c) is cultivated under the condition of 30-50°C (preferably 35-45°C).

[0093] In another preferred example, the bacteri...

Embodiment 1

[0125] Isolation of flocculant producing bacteria

[0126] The sample is the activated sludge collected from Xipian Sewage Treatment Plant in Wenzhou, Zhejiang. Add 1g of activated sludge to 99ml of sterile water and mix it evenly, then dilute it with sterile water to a weight (g) volume (ml) ratio of 10 -4 、10 -5 、10 -6 Three dilutions were spread and inoculated on the LB plate, and after culturing at 35°C for 48 hours, a single colony was picked and streaked on the LB plate for purification until it was a pure culture by microscopic examination, and then transferred to the LB slant after culture. Store at 4°C.

[0127] The isolated pure strains were inoculated into flocculant-producing medium (glucose 20g, K 2 HPO 4 5g, KH 2 PO 4 2g, (NH 4 ) 2 SO 4 0.2g, NaCl 0.1g, urea 0.5g, yeast extract 0.5g, MgSO 4 0.2g, H 2 (21000ml, pH7.5), shake and culture at 35°C and 160rpm for 72h, centrifuge at 8000rpm for 10min to obtain the supernatant, measure the flocculation activi...

Embodiment 2

[0130] Compound mutagenesis of Bacillus sp.WZ01

[0131]Insert the bacterial strain Bacillus sp.WZ01 obtained in Example 1 into a 250ml Erlenmeyer flask equipped with 50ml LB medium, vibrate at 35°C and 150rpm for 24h to activate, then transfer to In a 250ml Erlenmeyer flask with 50ml of LB medium, shake culture at 35°C and 150rpm until logarithmic phase (about 18h). Take 50ml of bacterial liquid and centrifuge at 5000rpm for 10min, wash the bacteria twice with normal saline, and then make 10 8 pcs ml -1 left and right bacterial suspensions. Take 15ml of bacterial suspension in a plate for ultraviolet mutagenesis. The power is 15W UV ultraviolet lamp, the irradiation distance is 20cm, and the irradiation time is 18min. Take 5ml of the bacterial suspension irradiated by ultraviolet light and inoculate it into a 250ml Erlenmeyer flask filled with 50ml of LB medium wrapped in black paper, and shake and cultivate at 40°C and 150rpm for 4-6h.

[0132] Centrifuge the bacterial ...

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Abstract

The invention discloses a Bacillus sp., and a method for producing flocculating agent through composite wastewater cultivation of the Bacillus sp.. The preservation number of the Bacillus sp. is CGMCC NO.9143. Fermentation cultivation of the Bacillus sp. with bean product wastewater and / or monosodium glutamate wastewater as a medium is carried out to obtain the polysaccharide flocculating agent. The Bacillus sp. and the flocculating agent production method enable the flocculating agent to be highly yielded and COD and NH4<+>-N in the bean product wastewater and the monosodium glutamate wastewater to be removed; and the obtained flocculating agent can improve the concentrated sludge dehydration performance, and can also remove Karenia mikimotoi in a Karenia mikimotoi cultivation solution and Alexandrium tamarense in a Alexandrium tamarense cultivation solution.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the breeding of a flocculant-producing bacillus and a method for compounding monosodium glutamate waste water and bean product waste water to cultivate the bacillus to produce flocculant. Background technique [0002] Bioflocculants are highly efficient, non-toxic and biodegradable, and are typical environmentally friendly functional materials, representing one of the important research and development directions of flocculants. Burterfield first screened flocculant-producing microorganisms from activated sludge in 1935. Since the 1970s, domestic and foreign scholars have screened many flocculant-producing microorganisms, and found that microorganisms with flocculation ability can be isolated from soil, activated sludge, domestic sewage, industrial wastewater and various sediment samples. Including bacteria, algae, actinomycetes, fungi and yeast, etc. At the end of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C02F3/34
CPCC02F3/34C02F2103/32C12P19/04C12N1/205C12R2001/07
Inventor 董新姣周茂洪葛世玫
Owner WENZHOU UNIVERSITY
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