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Composite enzymatic hydrolysis method of protein in degreased plant meal

A technology of protein compounding and plant meal, which is applied in the direction of fermentation, can solve the problems of lack of universal plant protein enzymatic hydrolysis methods, and achieve the effects of easy popularization and application, equipment standardization, and process stability

Inactive Publication Date: 2015-01-28
HEBEI ZIFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are all aimed at a certain plant protein, lacking a general plant protein enzymatic hydrolysis method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Pulverize 10 kg of defatted peanut meal and pass through a 100-mesh sieve.

[0026] 2) Add 50kg of water to the crushed peanut meal, place it in an extraction tank, stir at 50°C, add 50g of compound enzyme, the compound enzyme contains subtilisin, bromelain and papain, and the ratio is 7:2: 1. Extract and enzymatically hydrolyze for 120 minutes, centrifuge after completion, and collect the supernatant.

[0027] 3) Boil the supernatant collected in step 2), inactivate the enzyme, and collect the supernatant by centrifugation.

[0028] 4) Concentrating and drying the supernatant collected in step 3) to obtain 3.28 kg of the polypeptide product.

Embodiment 2

[0030] 1) Pulverize 10 kg of defatted soybean meal and pass through a 100-mesh sieve.

[0031] 2) Add 70kg of water to the crushed soybean meal, place it in an extraction tank, stir at 60°C, add 30g of compound enzyme, the compound enzyme contains subtilisin, bromelain and papain, and the ratio is 10:3: 3. Extract and enzymatically hydrolyze for 150 minutes, centrifuge after completion, and collect the supernatant.

[0032] 3) Boil the supernatant collected in step 2), inactivate the enzyme, and collect the supernatant by centrifugation.

[0033] 4) Concentrating and drying the supernatant collected in step 3) to obtain 2.87 kg of the polypeptide product.

Embodiment 3

[0035] 1) Pulverize 10 kg of defatted wheat germ meal and pass through a 100-mesh sieve.

[0036] 2) Add 40kg of water to the crushed wheat germ meal, place it in an extraction tank, stir at 50°C, add 70g of compound enzyme, the compound enzyme contains subtilisin, bromelain and papain, and the ratio is 8:2 :3, extract and enzymatically hydrolyze for 90 minutes, centrifuge after completion, and collect the supernatant.

[0037] 3) Boil the supernatant collected in step 2), inactivate the enzyme, and collect the supernatant by centrifugation.

[0038] 4) Concentrating and drying the supernatant collected in step 3) to obtain 2.76 kg of the polypeptide product.

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PUM

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Abstract

The invention provides a composite enzymatic hydrolysis method of protein in degreased plant meal. The composite enzymatic hydrolysis method is characterized by comprising the following steps: 1) pretreatment: crushing the degreased plant meal, and sieving; 2) extraction and enzymatic hydrolysis: carrying out extraction and composite enzymatic hydrolysis on the crushed degreased plant meal, and separating solid and liquid; 3) enzyme inactivation: carrying out enzyme inactivation on a supernatant obtained in the second step, and separating solid and liquid; and 4) concentrating and drying: concentrating and drying the supernatant obtained in the third step to obtain a polypeptide product. By being detected, the polypeptide product has angiotensin-converting enzyme inhibiting activity (anti-hypertension activity), antioxidant activity, antibacterial property and anti-inflammation activity and immunity improving activity. The composite enzymatic hydrolysis method is simple in process, is easy to control, is strong in strength and is easy in large scale production.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to a method for compound enzymatic hydrolysis of proteins in defatted vegetable dregs Background technique [0002] At present, there are many kinds of oils extracted from plants, so there are many defatted vegetable meal resources worthy of further development. There are a large number of biologically active polypeptides in the plant protein isolate prepared from plant defatted meal. The activity types of these polypeptides are very rich, which are closely related to the protein source, the type of enzyme used, the preparation process, the molecular weight range and the sequence length. At present, the biologically active types of peptides degraded from proven plant isolates include: inhibition of angiotensin-converting enzyme activity, inhibition of platelet aggregation activity, antioxidant activity, anti-tumor activity, antibacterial activity, anti-inflammatory activity, im...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 杨子丰孔英俊张贵锋马啸宇张泽玉陈静康跻耀
Owner HEBEI ZIFENG BIOTECH
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