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Method for simultaneously determining multiple preservatives in exported vegetables

A method of determination and technology of preservatives, applied in the field of chemical analysis and detection, can solve the problems of vegetable preservation quality decline, economic loss, easy spoilage and mildew, etc., and achieve short time consumption, high accuracy and precision, and simple detection steps Effect

Active Publication Date: 2015-01-28
重庆海关技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-distance transportation of inland vegetables for export is prone to spoilage and mildew. Irregular use or abuse of vegetable preservatives may lead to such drugs exceeding the standard and endangering people's health
Numerous testing items and a long testing cycle not only bring inconvenience to the purchase of fresh vegetable raw materials and export customs clearance, but also lead to a decline in the quality of vegetable preservation and economic losses, etc.

Method used

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  • Method for simultaneously determining multiple preservatives in exported vegetables
  • Method for simultaneously determining multiple preservatives in exported vegetables
  • Method for simultaneously determining multiple preservatives in exported vegetables

Examples

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Embodiment Construction

[0053] The specific implementation of the present invention will be further described below in conjunction with the examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0054] 1) extract

[0055] Use a homogenizer to make vegetable homogenate from the original vegetables, weigh 10.00g vegetable homogenate (accurate to 0.01g) into a 50mL offline tube, add 20mL n-hexane-ethyl acetate (1+2, V / V) solution , vortex and mix for 2 minutes, shake horizontally for 5 minutes, then add 5.00g (accurate to 0.01g) sodium chloride, vortex and mix again for 1min, 5000r / min offline for 3min, draw 10mL supernatant Ⅰ into a 15mL offline tube, To be purified.

[0056] 2) Purify the sample

[0057] Weigh 100mg of ethylenediamine-N-propylsilane (PSA, 60μm) and 500mg of anhydrous sodium sulfate into 10mL of supernatant Ⅰ, vortex and mix for 2min, 5000r / min off-line for 3min, draw supernatant Ⅱ into 25mL...

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Abstract

The invention discloses a method for simultaneously determining multiple preservatives in exported vegetables. The method comprises steps of extracting, purifying, concentrating, ultra performance liquid chromatography-mass spectrum / mass spectrum (UPLC-MS / MS) determination, etc. The method can simultaneously determine eighteen preservatives including imazalil, chlorpyrifos, carbendazol, thiabendazole, prochloraz, pyrimethanil, myclobutanil, flusilazole, propiconazole, triadimefon, iprodione, fenbuconazole, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, butyl p-hydroxybenzoate, ethyl naphthol and p-phenylphenol. The method for simultaneously determining multiple preservatives in exported vegetables disclosed by the invention has the advantages of simple and convenient steps, high accuracy and high precision.

Description

technical field [0001] The invention belongs to the field of chemical analysis and detection, and in particular relates to a simultaneous determination method of multiple preservatives in export vegetables. Background technique [0002] Vegetables are one of the essential foods in people's daily diet. They can provide various vitamins and minerals necessary for the human body, and play an important role in the residents' diet. Eating more vegetables has many functions such as health care and disease prevention, cancer prevention and treatment, nutrition enhancement, digestion promotion, and blood vessel softening. However, during field production, vegetables are often infected by microorganisms such as fungi and pathogenic bacteria, resulting in easy mildew and corruption after picking. Therefore, preservatives play an important role after picking vegetables. Preservatives are often made into various preparations or sprayed on the surface of vegetables, or directly soaked i...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/14
Inventor 李贤良曹淑瑞郗存显唐柏彬周雪张雷王国民
Owner 重庆海关技术中心
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