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Method for determinating seven kinds of quinolone residues in animal-source food through membrane dialysis-high performance liquid chromatography and tandem mass spectrometry

A technology of high-performance liquid chromatography and tandem mass spectrometry, which is applied to measuring devices, analytical materials, instruments, etc., to achieve the effects of simple sample processing, good reproducibility, and high sensitivity

Inactive Publication Date: 2015-01-28
威海出入境检验检疫局检验检疫技术中心 +1
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Problems solved by technology

However, there is no report on the combination of dialysis technology and analytical instruments for the detection of quinolone drug residues.

Method used

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  • Method for determinating seven kinds of quinolone residues in animal-source food through membrane dialysis-high performance liquid chromatography and tandem mass spectrometry
  • Method for determinating seven kinds of quinolone residues in animal-source food through membrane dialysis-high performance liquid chromatography and tandem mass spectrometry
  • Method for determinating seven kinds of quinolone residues in animal-source food through membrane dialysis-high performance liquid chromatography and tandem mass spectrometry

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Embodiment Construction

[0015] In order to specifically illustrate the present invention, the following examples are used for specific illustration, but the present invention is not limited thereto.

[0016] 1. Preparation of Dialysis Bags

[0017] Cut the dialysis membrane into small pieces of appropriate length (about 10cm), wash the dialysis membrane thoroughly with distilled water, put the unused dialysis membrane back into the storage solution, keep it sealed, and wear gloves when touching the dialysis membrane. When in use, one end is clamped with a sinking dialysis bag clip, and after filling with water, pressurize properly with your fingers to check that there is no leakage before loading the sample. Usually, one-third to one-half of the space should be reserved to prevent excessive water and buffer solution outside the bag from entering the bag during dialysis and bursting the bag. After loading the sample, clamp the mouth of the bag with a floating clamp, and put a glass bead in the bag to...

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Abstract

The invention belongs to the field of chemical analysis, and relates to a method for determinating seven kinds of quinolone residues in animal-source food through membrane dialysis-high performance liquid chromatography and tandem mass spectrometry. Through a membrane dialysis technology, a high performance liquid chromatography and tandem mass spectrometry (HPLC-MS / MS) determination method for determinating the seven kinds of quinolone residues in the animal-source food at the same time is established. A standard regenerated cellulose membrane (RC, molecular weight cut off (MWCO): 1000D) as a dialysis membrane, drug residues penetrate into dialysate through dialysis, and the dialysate is extracted by using chloroform; by the method, all quinolone drugs have good linearity within the concentration range of 5.0-100.0 micrograms / kg and have the limit of quantitation of 5.0 micrograms / kg; at three concentration adding levels of 5.0, 10.0 and 25.0 micrograms / kg, the average recycling ratio is 65.5-84.1%; the variable coefficient is 4.3-11.6%. The method is high in sensitivity, good in reproducibility and economical, and can be applied to determination and confirmation of the seven kinds of the quinolone residues in the animal-source food.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a detection method for quinolones in animal-source foods, in particular to a method for determining seven kinds of quinolone residues in animal-source foods by using membrane dialysis-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] Quinolones (QNs) are a class of synthetic antibacterial drugs with strong antibacterial activity and broad antibacterial spectrum. In recent years, quinolones have been widely used as antibacterial drugs in the animal breeding industry, and the US FDA has stipulated the limit of some QNs in poultry meat. The only QNs approved for use in Japan are enrofloxacin, difloxacin, orbifloxacin, danofloxacin, marbofloxacin, ofloxacin, and oxolinic acid. In Regulation No.508 / 1999 published in March 1999, the European Union stipulated the maximum residue limits of danofloxacin, flumequine, marbofloxacin, enrofloxaci...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 杨丽君徐成钢梁君妮王美玲宋晓华安代志
Owner 威海出入境检验检疫局检验检疫技术中心
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