Method for extracting antrodia camphorata oil from antrodia camphorata sporophore
An Antrodia camphorata oil and extraction technology, applied in the field of bioengineering, can solve problems such as the rareness of Antrodia camphorata oil, and achieve the effects of optimizing extraction and separation pressure, avoiding oil oxidation, and avoiding contact.
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Embodiment 1
[0025] A method for extracting Antrodia camphorata oil from Antrodia camphorata mycelium, comprising the following steps:
[0026] (1) Sample pretreatment: take 3g of Antrodia camphorata, rinse it, dry and grind it into powder, add it to the slant medium and shaker medium successively for cultivation, the cultivation temperature is 3 days, and the cultivation temperature is 24°C to obtain Antrodia camphorata Fungal mycelium fermentation broth;
[0027] (2) Vacuum-concentrate the mycelium fermentation liquid in step (1) to 1 / 4 of the original volume, dry to constant weight, and crush through an 80-mesh sieve;
[0028] (3) Extraction of Antrodia camphorata oil by SC-CO2 method: Put the crushed and sieved mycelium powder in step (1) into the extraction kettle of a supercritical CO2 extraction device, and add 15% mycelium powder entrained Antrodia camphorata oil was extracted with the same agent, and after the extraction, it was successively transferred to separation kettle I and...
Embodiment 2
[0036] A method for extracting Antrodia camphorata oil from Antrodia camphorata mycelium, comprising the following steps:
[0037] (1) Sample pretreatment: Take 5 g of Antrodia camphorata, rinse it, dry and grind it into powder, and add it to the slant medium and shaker medium successively for cultivation. The cultivation temperature is 5 days, and the cultivation temperature is 26°C. Fermented liquid of Zhi fungus mycelium;
[0038] (2) Vacuum-concentrate the mycelium fermentation liquid in step (1) to 1 / 5 of the original volume, dry to constant weight, and crush through a 100-mesh sieve;
[0039] (3) Extraction of Antrodia camphorata oil by SC-CO2 method: Put the crushed and sieved mycelium powder in step (1) into the extraction kettle of a supercritical CO2 extraction device, and add 35% mycelium powder entrained Extraction of Antrodia camphorata oil, after extraction, successively transferred to separation kettle I and separation kettle II for separation, the separated mi...
Embodiment 3
[0047] A method for extracting Antrodia camphorata oil from Antrodia camphorata mycelium, comprising the following steps:
[0048] (1) Sample pretreatment: Take 4 g of Antrodia camphorata, rinse it, dry and grind it into powder, and then add it to the slant medium and shaker medium for cultivation. The cultivation temperature is 4 days, and the cultivation temperature is 25 ° C. Fermented liquid of Zhi fungus mycelium;
[0049] (2) Vacuum-concentrate the mycelium fermentation liquid in step (1) to 1 / 4 of the original volume, dry to constant weight, and crush through an 80-mesh sieve;
[0050] (3) Extraction of Antrodia camphorata oil by SC-CO2 method: Put the crushed and sieved mycelium powder in step (1) into the extraction kettle of a supercritical CO2 extraction device, and add 30% mycelium powder entrained Extraction of Antrodia camphorata oil, after extraction, successively transferred to separation kettle I and separation kettle II for separation, the separated mixed so...
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