Method for extracting antrodia camphorata oil from antrodia camphorata sporophore

An Antrodia camphorata oil and extraction technology, applied in the field of bioengineering, can solve problems such as the rareness of Antrodia camphorata oil, and achieve the effects of optimizing extraction and separation pressure, avoiding oil oxidation, and avoiding contact.

Inactive Publication Date: 2015-02-04
中山安荞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Based on the current development of Antrodia camphorata, there is an urgent need to seek an extraction method of Antrodia camphorata oil to meet the renewal and development of Antrodia camphorata products. At present, there are few reports on the extraction method of Antrodia camphorata oil, and there are certain defects in the existing reports. For this reason, the present invention provides a kind of method of extracting Antrodia camphorata oil with mycelia of Antrodia camphorata

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting Antrodia camphorata oil from Antrodia camphorata mycelium, comprising the following steps:

[0026] (1) Sample pretreatment: take 3g of Antrodia camphorata, rinse it, dry and grind it into powder, add it to the slant medium and shaker medium successively for cultivation, the cultivation temperature is 3 days, and the cultivation temperature is 24°C to obtain Antrodia camphorata Fungal mycelium fermentation broth;

[0027] (2) Vacuum-concentrate the mycelium fermentation liquid in step (1) to 1 / 4 of the original volume, dry to constant weight, and crush through an 80-mesh sieve;

[0028] (3) Extraction of Antrodia camphorata oil by SC-CO2 method: Put the crushed and sieved mycelium powder in step (1) into the extraction kettle of a supercritical CO2 extraction device, and add 15% mycelium powder entrained Antrodia camphorata oil was extracted with the same agent, and after the extraction, it was successively transferred to separation kettle I and...

Embodiment 2

[0036] A method for extracting Antrodia camphorata oil from Antrodia camphorata mycelium, comprising the following steps:

[0037] (1) Sample pretreatment: Take 5 g of Antrodia camphorata, rinse it, dry and grind it into powder, and add it to the slant medium and shaker medium successively for cultivation. The cultivation temperature is 5 days, and the cultivation temperature is 26°C. Fermented liquid of Zhi fungus mycelium;

[0038] (2) Vacuum-concentrate the mycelium fermentation liquid in step (1) to 1 / 5 of the original volume, dry to constant weight, and crush through a 100-mesh sieve;

[0039] (3) Extraction of Antrodia camphorata oil by SC-CO2 method: Put the crushed and sieved mycelium powder in step (1) into the extraction kettle of a supercritical CO2 extraction device, and add 35% mycelium powder entrained Extraction of Antrodia camphorata oil, after extraction, successively transferred to separation kettle I and separation kettle II for separation, the separated mi...

Embodiment 3

[0047] A method for extracting Antrodia camphorata oil from Antrodia camphorata mycelium, comprising the following steps:

[0048] (1) Sample pretreatment: Take 4 g of Antrodia camphorata, rinse it, dry and grind it into powder, and then add it to the slant medium and shaker medium for cultivation. The cultivation temperature is 4 days, and the cultivation temperature is 25 ° C. Fermented liquid of Zhi fungus mycelium;

[0049] (2) Vacuum-concentrate the mycelium fermentation liquid in step (1) to 1 / 4 of the original volume, dry to constant weight, and crush through an 80-mesh sieve;

[0050] (3) Extraction of Antrodia camphorata oil by SC-CO2 method: Put the crushed and sieved mycelium powder in step (1) into the extraction kettle of a supercritical CO2 extraction device, and add 30% mycelium powder entrained Extraction of Antrodia camphorata oil, after extraction, successively transferred to separation kettle I and separation kettle II for separation, the separated mixed so...

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Abstract

The invention relates to a method for extracting antrodia camphorata oil from antrodia camphorata sporophore. The method comprises the following steps: firstly drying and grinding antrodia camphorata, and successively adding into a slant medium and a shaking medium for culture, so as to obtain an antrodia camphorata sporophore fermentation liquid; then performing vacuum condensation on the sporophore fermentation liquid to have a volume accounting for 1/4-1/5 of the original volume, drying to obtain a constant weight, crushing and sieving; and finally packing the sporophore subjected to crushing and sieving into an extraction kettle of a supercritical CO2 extraction apparatus, adding an entrainer to extract antrodia camphorata oil, after extraction, successively transferring into a separation kettle I and a separation kettle II for separation, performing centrifugal separation on the mixed liquid subjected to separation at a centrifugation speed of 5000-8000 r/min, and collecting antrodia camphorata oil. According to the method, the sporophore fermentation liquid is condensed under the condition of vacuum, so that grease oxidation is avoided, and the sample is guaranteed to be condensed at a relatively low temperature. Temperature and pressure for extraction and separation are optimized and limited, so that maximum production and sufficient extraction and separation of antrodia camphorata oil are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for extracting antrodia camphorata oil from fruiting bodies of antrodia camphorata. Background technique [0002] Antrodia camphorata, also known as Antrodia camphorata or Antrodia camphorata, is a unique and precious fungus in Taiwan. The surface of Antrodia camphorata is very red, and there are spores inside. Its fruiting bodies have no stipe and have a strong aroma. Antrodia camphorata is mainly distributed in Taiwan, my country, and rarely seen in other regions. In recent years, studies have found that Antrodia camphorata has complex components and contains many physiologically active substances with various physiological activities, such as anti-oxidation, liver protection, anti-cancer, detoxification, anti-inflammatory, etc. It has a miraculous effect on food poisoning and other critical diseases. Since the news of the miraculous curative effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B1/10C11B3/00
CPCC11B1/025C11B1/104C11B3/006C11B3/16
Inventor 刘巴宁郑安乔
Owner 中山安荞生物科技有限公司
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