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Interfering RNA for inhibiting and treating echinococcus granulosus disease and application thereof

A technology for echinococcosis and echinococcosis, which is applied in DNA/RNA fragments, recombinant DNA technology, medical preparations containing active ingredients, etc., can solve unsatisfactory side effects and unsatisfactory killing effects , anaphylactic shock and other problems

Inactive Publication Date: 2015-02-18
吕海龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, extensive research has been done on 95% ethanol, hypertonic saline, 5% formalin, silver nitrate, albendazole and other drugs and their combined effects in vitro, in animals and clinically. , although they can play a certain effect, they are not ideal and have relatively large side effects: 95% ethanol can cause serious hepatobiliary complications in the body, and 20% hypertonic saline can cause serious hepatobiliary complications in the patient. 10%H 2 o 2 It will cause air embolism and even anaphylactic shock in the body. Serious hepatobiliary complications will occur after formalin use. 20% silver nitrate can cause intraoperative shock, cardiovascular failure, and renal failure in patients. Side effects such as hepatic insufficiency, postoperative dyspnea, cyanosis, hypotension and a series of side effects, after clinical use of albendazole (ABZ), the level of liver enzymes will increase. It can effectively kill, but it cannot be applied in the human body, and the killing effect of low-concentration acidic and alkaline solutions is not ideal
[0005] According to the cognition of the pharmaceutical field, the ideal local chemotherapy drug should have a rapid and complete drug action effect (without limitations and side effects), from this point of view, no ideal local chemotherapy drug has been found yet

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  • Interfering RNA for inhibiting and treating echinococcus granulosus disease and application thereof
  • Interfering RNA for inhibiting and treating echinococcus granulosus disease and application thereof
  • Interfering RNA for inhibiting and treating echinococcus granulosus disease and application thereof

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Embodiment Construction

[0020] In order to illustrate the effect of the interfering RNA of the present invention, the applicant carried out an in vitro killing experiment of Echinococcus granulosa, and the specific experimental process is as follows:

[0021] Experiment 1: Collection and culture of Echinococcus granulosa protosegments

[0022] Take the fresh sheep liver naturally infected with cystic hepatic hydatid disease, wash the surface of the sheep liver several times until it is clean, and then disinfect it with 75% alcohol. The protoscapular segment was washed with PBS (PH=7.2) for several times until it was clear, and 0.1% eosin staining solution was used for staining rejection reaction, and more than 98% were rejected. The protosegments were observed with the naked eye as white fine sand-like uniform particles. The protosegments with good activity settled faster, while those with poor activity or dead settled relatively slowly. Under the inverted microscope, the protozoan body was concave,...

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Abstract

The invention discloses an interfering RNA for inhibiting and treating an echinococcus granulosus disease. The interfering RNA targets a GRP78 gene of echinococcus granulosus and has the target sequence of CCTCGTGGTTTACCTCAAA. By transfecting protoscoleces of echinococcus granulosus through electroporation, siRNA-GRP78 provided by the invention can destroy structures of the protoscoleces of cystic echinococcosis, induce apoptosis of the protoscoleces and reduce the activities of the protoscoleces so as to inhibit and kill the protoscoleces of echinococcus granulosus.

Description

technical field [0001] The invention relates to an interfering RNA and its application for inhibiting and treating echinococcosis granulosus, and belongs to the field of biomedical engineering. Background technique [0002] Echinococcosis (echinococcosis), commonly known as hydatid disease, is a zoonotic disease that seriously endangers human health and the development of animal husbandry caused by the parasitic larvae of Echinococcus tapeworm in the middle tapeworm stage. suffering from parasitic diseases. There are many types of echinococcosis, currently in my country there are mainly two types of echinococcosis (cystic echinococcosis, CE) and echinococcosis (alveolar echinococcosis, AE). , which were caused by Echinococcus granulosus (E.g) and Echinococcus multilocularis (E.m) parasitizing in humans and some animals. Echinococcus granulosus has a global distribution and is mainly prevalent in Xinjiang, Qinghai, Inner Mongolia, Tibet and other provinces with developed ani...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K31/7088A61P33/10
Inventor 吕海龙姜玉峰裴学莲
Owner 吕海龙
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