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Method for identifying age of ginseng by utilizing length of ginseng telomere

A technology of ginseng and age, which is applied in the direction of biochemical equipment and methods, microbe measurement/inspection, etc., can solve the problem that the identification and quantification of ginseng age cannot be recognized by others

Active Publication Date: 2015-02-18
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, no matter which kind of identification has commercial exaggeration in it, the real quantitative and digital research on the identification of ginseng age is not systematic and cannot be recognized by people

Method used

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  • Method for identifying age of ginseng by utilizing length of ginseng telomere
  • Method for identifying age of ginseng by utilizing length of ginseng telomere
  • Method for identifying age of ginseng by utilizing length of ginseng telomere

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Effect test

Embodiment 1

[0048] Example 1. Establishment of a method for identifying the age of ginseng by using ginseng telomere length

[0049] Ginseng tested: ginseng samples of different years from two regions, see Table 1 for details.

[0050] Table 1 Ginseng samples of different years in two regions

[0051]

[0052] 1. Extraction of Ginseng Genomic DNA

[0053] Genomic DNA of each tested ginseng was extracted by CTAB method. details as follows:

[0054] Take 10-50 mg dry weight (without mildew) of the ginseng to be tested located 1-2 cm below the reed head, put it in a pulverizer, grind it, and pass it through a 40-mesh sieve. Transfer the powder to a 2.0mL Eppendorf tube, add 900μL of sterilized CTAB extract (recipe: 2% (2g / 100ml) CTAB, 100mmol / L Tris-HCl pH=8.0, 20mmol / L EDTA, 1.4mol / L NaCl), 0.02g PVP 40000, 10μL β-mercaptoethanol, fully vortexed and mixed, 65°C water bath for 1.5h-2h, and gently shaked 2-3 times during the period. After completion, take it out and cool to room temp...

Embodiment 2

[0076] Example 2, the application of the method of identifying the age of ginseng by using the telomere length of ginseng

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Abstract

The invention discloses a method for identifying the age of ginseng by utilizing the length of a ginseng telomere. The method comprises the following steps: (A) establishing a mathematical model: (a1) selecting a plurality of samples from the positions 1-2 cm below the rhizomes of the ginseng of known age and extracting genomic DNA from each sample; (a2) taking each genomic DNA as a template, conducting qPCR on a primer pair distinguished from the ginseng telomere to obtain a Ct value and marking the Ct value as T, and conducting qPCR on a primer pair distinguished from a single copy gene of the ginseng to obtain another Ct value and marking the Ct value as S; (a3) calculating the T / S ratio for each ginseng; (a4) taking the ages of the ginseng as the abscissa values and taking all the calculated T / S ratios as the ordinate values to draw a standard linear curve and obtain a curve equation; (B) adopting the mathematical model to identify the age of ginseng to be measured: obtaining the T / S ratio for the ginseng to be measured according to the operations from step (a1) to step (a3), and substituting the T / S ratio into the mathematical model to obtain the age of the ginseng to be measured. The method has the advantages that the identified age of ginseng is highly identical with the actual age of the ginseng, the cost is low, and the flux is high. Compared with the traditional Southern blot technology adopted for measuring the length of a ginseng telomere, the method provided by the invention has less demand for DNA and a shorter test period.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for identifying the age of ginseng by using the telomere length of ginseng, in particular to a method for identifying the age of ginseng according to the relative telomere length of ginseng by using the qPCR method. Background technique [0002] Ginseng is the dry root and rhizome of Panax ginseng C.A.Mey., a plant of Araliaceae. Among many herbal plants, ginseng is famous for its long life and high price, and is known as the king of all herbs. The greatest value of ginseng lies in its medicinal efficacy. As early as in "Shen Nong's Herbal Classic", there is a description of the magical effect of ginseng. In today's society, ginseng appears in the market as a cultural symbol or as a collectible. Ginseng with a long life and good root type is sought after by people. A small ginseng may cost tens to several million yuan. [0003] The key to the price of ginseng lies in the le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q2531/113C12Q2537/165C12Q2563/107
Inventor 黄璐琦蒋超袁媛王尧龙
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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