Secondary proliferation culture method of color osmanthusfragrans test-tube plantlets

A technology of subculture multiplication and cultivation method, which is applied in the field of subculture multiplication and cultivation of Caigui test-tube seedlings, can solve the problems of vastly different technical methods, poor growth uniformity of cutting seedlings, limited production, etc., and achieve the goal of making up for defects and fast propagation speed Effect

Inactive Publication Date: 2015-02-25
上海绿力农业科技有限公司
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Problems solved by technology

[0004] The propagation of five-color osmanthus generally adopts twig cuttings. The production is limited by seasonal changes and its cutting propagation requires a large number of cuttings. Due to the inconsistency of cutting sources, the growth uniformity of cutting seedlings may be worse than that of tissue culture s

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  • Secondary proliferation culture method of color osmanthusfragrans test-tube plantlets

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Embodiment Construction

[0018] The present invention is further illustrated by the following examples.

[0019] Caigui test-tube plantlet subculture proliferation culture method of the present invention, it comprises the steps:

[0020] Use the newly germinated shoots of Caigui as explants, after sterilizing the explants, cut them into 1-1.5cm segments under aseptic conditions, inoculate them into the axillary bud induction medium, and cultivate them for 4-5 weeks to produce Axillary buds: cut the axillary buds into 1-1.5cm terminal buds or stem segments, inoculate them into the same medium for subculture and multiplication culture, and cultivate for 4-5 weeks to obtain clustered buds; the above-mentioned cultivation process is under 14 hours of light every day , The photosynthetically active photon beam density is 40-50μmol / m 2 s and a temperature of 24 to 26°C, the axillary bud induction medium or subculture medium consists of basic medium, cytokinin, agar 5.5g / L and white sugar 30g / L, and adj...

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Abstract

The present invention relates to a secondary proliferation culture method of color osmanthusfragrans test-tube plantlets. The method comprises: adopting the newly-germinating shoots of the color osmanthusfragrans as the explants, carrying out disinfection sterilization on the explants, cutting into the small segments with the length of 1-1.5 cm under a sterile condition, inoculating into an axillary bud induction culture medium, culturing for 4-5 weeks to produce axillary buds, cutting the axillary buds into the terminal bud or stem segment with the length of 1-1.5 cm, and inoculating into the same culture medium to carry out secondary proliferation culture for 4-5 weeks to obtain the clustered shoots, wherein the culture process is performed under the conditions of the lighting time of 14 h each day, the photosynthetically active photon flux density of 40-50 [mu]mol/m<2>.s and the temperature of 24-26 DEG C, the axillary bud induction culture medium comprises a base culture medium, 0.1-0.5 mg/L of TDZ, 0.1-1.0 mg/L of KT, 5.5 g/L of agar, and 30 g/L of white granulated sugar, and the pH value is adjusted to 5.8. The secondary proliferation culture method of the present invention has characteristics of rapid breeding and no limitation of the season change on the production. In addition, the defects of the cutting seedlings are effectively overcome.

Description

technical field [0001] The invention relates to the technical field of rapid propagation of tissue-cultured test-tube plantlets, in particular to a method for subculture and cultivation of test-tube plantlets of Caigui. Background technique [0002] Caigui is an evergreen small tree or shrub belonging to Oleaceae (Oleaceae). The unique color change of Caigui leaves shows its high ornamental value. As an important garden and economic tree species in my country, it has a broad market prospect. Especially the five-color leaf osmanthus among the colorful osmanthus varieties. [0003] The majority of Osmanthus plants are ornamental flowers and trees and excellent garden tree species in China. Among them, osmanthus is one of the top ten famous flowers in China, with good shape, color, fragrance and rhyme, and has high ornamental value. At present, there are varieties of Caiyegui in China, such as Yuntian Caigui and Yinbian Caigui. The leaves are finally in the state of mosaic lea...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈权肖建梅
Owner 上海绿力农业科技有限公司
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