Microorganism compound preparation and application

A compound preparation and microbial technology, applied in the field of microorganisms, can solve the problems of single control effect, aggravation of sickle wilt or bacterial wilt, lack of disease-resistant varieties, etc., and achieve outstanding control effect

Inactive Publication Date: 2015-02-25
中国科学院上海生命科学研究院湖州现代农业生物技术产业创新中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such diseases often occur mixedly in the field. For example, root-knot nematode disease will greatly aggravate the occurrence of Fusarium wilt or bacterial wilt. There is a lack of disease-resistant varieties and high-efficiency and low-toxicity pesticides in production, so the loss is huge
[0003] During the implementation of biological control of soil-borne diseases, a variety of microbial agents have achieved certain control effects through measures such as mixed application with basal fertilizers and root irrigation, but there are still some The outstanding problems are: 1. Single control effect and poor broad spectrum; 2. Short duration of control effect; 3. High cost for continuous use

Method used

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  • Microorganism compound preparation and application

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Experimental program
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Effect test

Embodiment 1

[0019] 1. Microorganism composite preparation, which is prepared by the following method:

[0020] (1) Activation of strains

[0021] Bacillus subtilis Bacillus subtilis (Strain Collection No. CGMCCNo.5652), Bacillus cereus Bacillus cereus Culture preservation number (ATCC 11077), Bacillus licheniformis Bacillus licheniformis (Bacteria preservation number ATCC 11946) After the strain was taken out from the -80°C ultra-low temperature refrigerator, it was activated and cultured through 3 transfers, and the medium was LB solid medium;

[0022] (2) Preparation of seed bacteria solution

[0023] Pick a single colony separately, and culture it for 15 hours in a new liquid seed medium LB at 28°C and 180rpm;

[0024] (3) Inoculate into the liquid culture medium of the fermenter according to the inoculum amount of 0.5%;

[0025] The formula of the liquid medium is: by weight percentage, maltose 0.2%; corn flour, 0.5%; soybean flour, 0.4%; tryptone, 0.5%; CaCl 2 , 0.02%; MnSO ...

Embodiment 2

[0036] 1. Microorganism composite preparation, which is prepared by the following method:

[0037] (1) Activation of strains

[0038] Bacillus subtilis Bacillus subtilis (Strain Collection No. CGMCCNo.5652), Bacillus cereus Bacillus cereus Culture preservation number (ATCC 11077), Bacillus licheniformis Bacillus licheniformis (Bacteria preservation number ATCC 11946) After the strain was taken out from the -80°C ultra-low temperature refrigerator, it was activated and cultured through 3 transfers, and the medium was LB solid medium;

[0039] (2) Preparation of seed bacteria solution

[0040] Pick a single colony separately, and culture it in a new liquid seed medium LB at 37°C, 200rpm for 15 hours;

[0041] (3) Inoculate into the liquid culture medium of the fermenter according to the inoculum amount of 0.5%;

[0042] The formula of the liquid medium is: by weight percentage, maltose 0.5%; corn flour, 0.7%; soybean flour, 1%; tryptone, 0.5%; CaCl 2 , 0.02%; MnSO 4 , ...

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Abstract

The invention relates to a microorganism compound preparation, belonging to the technical field of microorganisms. The microorganism compound preparation is prepared from a compound bacterial agent, as a medical component, formed by compounding a fermentation culture solution of preparation (a)-bacillus subtilis with the strain preservation number of CGMCC No.5652, a fermentation culture solution of preparation (b)-bacilus cereus with the strain preservation number of ATCC11077 and a fermentation culture solution of preparation (c)-bacillus licheniformis with the strain preservation number of ATCC11946, and a pharmaceutically acceptable additive as an auxiliary; and the bacterial colony total quantity ratio in the prepared microorganism compound preparation is that the bacillus subtilis with the strain preservation number of CGMCC No.5652 to the bacillus licheniformis with the strain preservation number of ATCC11946 to the bacillus licheniformis with the strain preservation number of ATCC11946 is (40-180) to (3-15) to (9-33). The microorganism compound preparation can be used for effectively preventing and treating a plurality of soil-borne diseases of solanaceae plants and has the advantage of prominent prevention effect.

Description

technical field [0001] The invention relates to a microbial compound preparation for preventing and controlling soil-borne diseases of Solanaceae crops, belonging to the technical field of microorganisms. Background technique [0002] Common soil-borne diseases of Solanaceae crops mainly include fusarium wilt, blight, bacterial wilt, root-knot nematode and so on. Such diseases often occur mixedly in the field. For example, root-knot nematode disease will greatly aggravate the occurrence of Fusarium wilt or bacterial wilt. In production, there is a lack of disease-resistant varieties and high-efficiency and low-toxicity pesticides, resulting in huge losses. [0003] During the implementation of biological control of soil-borne diseases, a variety of microbial agents have achieved certain control effects through measures such as mixed application with basal fertilizers and root irrigation, but there are still outstanding problems: 1. Single control effect and poor broad-spectr...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00
Inventor 高丙利王成树李师默张鹏
Owner 中国科学院上海生命科学研究院湖州现代农业生物技术产业创新中心
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