Human oral squamous cell carcinoma cal‑27‑hcpt drug-resistant cell line

A CAL-27-HCPT, CAL-27 technology, applied in the field of CAL-27-HCPT drug-resistant cell lines of human oral squamous cell carcinoma, can solve problems such as gene changes, and achieve the effect of stable traits

Active Publication Date: 2017-08-15
上海尤里卡信息科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defect that the human oral squamous cell carcinoma drug-resistant cell line screened by the current conventional method is easy to cause genetic changes due to human factors, and to use human oral squamous cell carcinoma CAL-27 cells as parent cells, select Tumor chemotherapeutic drugs are commonly used as screening drugs, and the real action process of drugs after administration in the human body is simulated in vitro, so as to establish a stable and effective screening system and method for drug-resistant cell lines

Method used

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  • Human oral squamous cell carcinoma cal‑27‑hcpt drug-resistant cell line
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  • Human oral squamous cell carcinoma cal‑27‑hcpt drug-resistant cell line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Screening of human oral squamous cell carcinoma CAL-27 / 5-Fu drug-resistant cell lines

[0069] In this example, 5-fluorouracil (5-Fu) was used as a screening drug to induce and screen CAL-27 cells, to establish a CAL-27 / 5-Fu drug-resistant cell line, and to detect the characteristics of drug-resistant cells.

[0070] 1. Parental cell culture:

[0071] Human oral squamous cell carcinoma CAL-27 (ATCC NO. is CRL-2095) cells were grown in high-sugar DMEM medium containing 10% FBS, placed in 5% CO 2 , Cultured in a 37°C incubator.

[0072] 2. Parent cell IC 50 Determination of IC with 20 calculation

[0073] Drug: 5-Fluorouracil (5-Fu)

[0074] Dosage: Design 8 drug concentration gradients (0μg / ml, 0.02μg / ml, 0.04μg / ml, 0.08μg / ml, 0.16μg / ml, 0.32μg / ml, 0.64μg / ml, 1.28μg / ml), Measuring the IC of CAL-27 cells by MTT method 50 concentration. The full name of MTT is 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenyltetrazolium romide, and its Chinese chemical name ...

Embodiment 2

[0110] Example 2 Screening of human oral squamous cell carcinoma CAL-27 / CDDP drug-resistant cell lines

[0111] CAL-27 cells were screened by cisplatin (CDDP) drug induction, CAL-27 / CDDP drug-resistant cell lines were established, and the characteristics of drug-resistant cells were detected.

[0112] One, parent cell culture: operation is the same as embodiment 1.

[0113] 2. Parent cell IC 50 Determination of IC with 20 calculation

[0114] Drug: Cisplatin (CDDP)

[0115] Dosage: with embodiment 1.

[0116] The IC of CAL-27 cells was calculated by the modified Cole's method 50 The concentration was 2.333 μg / ml, and the IC was calculated 20 The concentration of 0.75 μg / ml was used as the initial concentration for screening.

[0117] 3. Induction of drug-resistant cell lines in human oral squamous cell carcinoma

[0118] Human oral squamous cell carcinoma CAL-27 cells in good growth state and in the logarithmic growth phase were collected and counted by trypsinization....

Embodiment 3

[0136] Example 3 Screening of human oral squamous cell carcinoma CAL-27-HCPT drug-resistant cell line

[0137] CAL-27 cells were screened by hydroxycamptothecin (HCPT) drugs, and CAL-27-CDDP drug-resistant cell lines were established, and the characteristics of drug-resistant cells were detected.

[0138] One, parent cell culture: operation is the same as embodiment 1

[0139] 2. Parent cell IC 50 Determination of IC with 20 calculation

[0140] Drug: Hydroxycamptothecin (HCPT)

[0141] Dosage: with embodiment 1.

[0142] The IC of CAL-27 cells was calculated by the modified Cole's method 50 The concentration is 0.101 μg / ml, and the IC is calculated 20 The concentration of 0.01 μg / ml was used as the initial concentration for screening.

[0143] 3. Induction of drug-resistant cell lines in human oral squamous cell carcinoma

[0144] Human oral squamous cell carcinoma CAL-27 cells in good growth state and in the logarithmic growth phase were collected and counted by tryp...

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Abstract

The invention discloses a human oral squamous carcinoma CAL-27-HCPT drug-resistant cell line. The human oral squamous carcinoma CAL-27-HCPT drug-resistant cell line has the collection number of CCTCC NO: C2012151. The drug-resistant cell line has the biological properties of clinical human oral squamous carcinoma and is an ideal model for researching the drug resistance mechanism of human oral squamous carcinoma cells.

Description

[0001] This application is a divisional application of the following applications: filing date, January 17, 2013; application number, 201310017722.3; invention title, culture method of human oral squamous cell carcinoma CAL-27 drug-resistant cell line and the cell line obtained from culture. technical field [0002] The invention belongs to the field of biotechnology, in particular to a CAL-27-HCPT drug-resistant cell line of human oral cavity squamous cell carcinoma. Background technique [0003] In my country, carcinoma is the most common malignant tumor of the oral and maxillofacial region, and sarcoma is rare. Among cancers, squamous cell carcinoma is the most common, generally accounting for more than 80%; followed by glandular epithelial carcinoma (such as mucoepidermoid carcinoma, adenocarcinoma, adenoid cystic carcinoma, malignant pleomorphic adenoma, acinar carcinoma) cell carcinoma, etc.) and undifferentiated carcinoma. Squamous cell carcinoma often metastasizes t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09A01K67/027
Inventor 王海峰洪谊姚远颋谈竹君劳昕元
Owner 上海尤里卡信息科技有限公司
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