Compound stabilizer used for lactic dehydrogenase kit
A composite stabilizer, lactate dehydrogenase technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of high implementation cost, instability, and complex methods, and can prolong the storage time and improve the stability. Sexual, easy to formulate effects
Active Publication Date: 2015-03-04
SUZHOU COMING CHENGYE MEDICAL TECH
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Problems solved by technology
In the patent CN200980105640, the coenzyme is chemically modified by adding glucose dehydrogenase to generate a relatively stable coenzyme derivative, so that it can achieve a stable effect without affecting the application of the reagent, but this method is costly and does not It is easy to promote; in the patent CN1179792A, an enzyme and a substrate are used to reduce the oxidized coenzyme to a reduced coenzyme, which facilitates the regeneration of the coenzyme during storage, but this method is costly to implement, and the added enzyme itself is also unstable; Patent CN02139298 by adding NAD + , using the enzyme-substrate-NAD + or enzyme-substrate-NADP + The system circulation converts the oxidized coenzyme into the reduced coenzyme, which can be measured when the reduced coenzyme accumulates to a certain concentration. Kit requirements; patent 200610030784.8 provides a composite stabilizer for NADH, but it can only prove that the composite stabilizer has a certain stabilizing effect on NADH that exists alone, and it does not mean that it can also be added to a kit with more complex components. have a similar stabilizing effect
Method used
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Embodiment 1
[0013] A compound stabilizer for lactate dehydrogenase kit is provided, the content of each component of the compound stabilizer is: Tris 6.06 g / L, EDTA-2Na 50 g / L, trehalose 25 g / L, β - Cyclodextrin 20 g / L, Glycerin 10 g / L, Polyvinylpyrrolidone 20 g / L, Proclin300 5 g / L. After the above-mentioned components are fully dissolved and mixed, a finished liquid composite stabilizer can be prepared.
[0014] Example 1 was applied to the lactate dehydrogenase assay kit, and evaluated from the aspects of stability, accuracy and usage.
[0015] (1) Stability and accuracy test
[0016] 1. Stabilizer for testing: composite stabilizer described in Example 1.
[0017] 2. The test kit is a lactate dehydrogenase assay kit, which consists of reagent 1 and reagent 2. The components are as follows:
[0018] Reagent 1: disodium hydrogen phosphate 25.069 g / L, potassium dihydrogen phosphate 2.435 g / L, α-ketobutyric acid sodium salt 0.496 g / L;
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The invention discloses a compound stabilizer used for a lactic dehydrogenase kit. The compound stabilizer comprises tris hydroxy methyl aminomethan, edetic acid disodium, mycose, beta-cyclodextrin, glycerol, polyvinylpyrrolidone and liquid biological preservatives Proclin300. By means of the mode, the compound stabilizer used for the lactic dehydrogenase kit comprises buffer agents, chelating agents, saccharides, surface active agents, the organic solvents and corrosion removers. After the compound stabilizer is added into the lactic dehydrogenase measurement kit, the stability of testing results can be obviously improved, and the retention time of the lactic dehydrogenase measurement kit can be effectively prolonged.
Description
technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a compound stabilizer used in a lactate dehydrogenase kit. Background technique [0002] Serum lactate dehydrogenase (LDH) is an oxidoreductase that catalyzes the interconversion reaction of pyruvate and lactate. Lactate dehydrogenase is divided into 5 isoenzymes according to subunits, and there are two types: muscle type and cardiac type. The activity of lactate dehydrogenase is used to evaluate the damage of cells or tissues, based on the conversion of reduced nicotinamide adenine dinucleotide (NADH) to oxidized nicotinamide adenine dinucleotide (NAD + ), causing the change of absorbance at 340nm wavelength, so as to quantitatively measure the activity of lactate dehydrogenase, the content of lactate dehydrogenase is proportional to the consumption of NADH, and NADH, as a coenzyme, its activity is susceptible to various factors Interference, and itself is easily oxidized, wh...
Claims
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IPC IPC(8): C12Q1/32
Inventor 周立霞杨灵勇王燕华李勋波
Owner SUZHOU COMING CHENGYE MEDICAL TECH