Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing specific egg yolk antibody against influenza A virus H3N2

A technology of chicken egg yolk antibody and influenza virus, which is applied in the field of preparation of specific chicken egg yolk antibody against influenza A (H3N2) virus, and can solve problems such as vaccine development lag

Inactive Publication Date: 2015-03-11
广东京豪生物制药有限公司
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control measures against human influenza A (H3N2) pandemic mainly use chemical drugs and vaccines, but the excessive use of chemical drugs often produces side effects and the development of vaccines often lags behind the outbreak of the epidemic and the disadvantages that vaccines can only prevent susceptible populations significantly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0006] Embodiment 1: Preparation of chicken egg yolk antibody specific to influenza A (H3N2) virus.

[0007] (1) Preparation of influenza A (H3N2) virus: inoculate the influenza A (H3N2) virus strain into MDCK cells grown to a monolayer, CO 2 Cultivate in the incubator until the cells appear cytopathic CPE++++, harvest, freeze and thaw three times at -20°C, centrifuge, add PBS to dilute the purified virus solution, determine the TCID50 value, inactivate at 56°C for 30 minutes, and store at 4°C.

[0008] (2) Preparation of influenza A H3N2 virus immune antigen: according to the TCID50 value, the above influenza A H3N2 virus was diluted with 0.01mol / L phosphate buffer saline PBS (pH=7.2), and mixed with complete Freund's adjuvant according to the 1:1 (V / V) fully emulsified to prepare immune antigen.

[0009] (3) Immune laying hens with H3N2 influenza virus immunization antigens: Take healthy laying hens of appropriate age, and use emulsified immune antigens on the muscles, In...

Embodiment approach 2

[0015] Embodiment 2: Detection of the purity of the specific chicken egg yolk antibody against influenza A (H3N2) virus.

[0016] The purity of chicken egg yolk antibody specific to H3N2 influenza virus was separated by cellulose acetate membrane electrophoresis. Cellulose acetate membrane electrophoresis is an electrophoresis method based on a cellulose acetate membrane as a support. Cellulose acetate membrane is made of cellulose diacetate. It has a uniform foam-like structure with a thickness of only 120 microns. It has strong permeability and no resistance to molecular movement. It is easy and fast to perform protein electrophoresis as a support for zone electrophoresis. It has the advantages of less sample consumption, wide application range, clear separation, and no adsorption phenomenon. It has been widely used in the separation and identification of serum proteins, lipoproteins, hemoglobin, glycoproteins, and isoenzymes. Test results show: purity ≥ 40%.

Embodiment approach 3

[0017] Embodiment 3: ELISA titer and activity detection of anti-influenza A H3N2 virus-specific chicken egg yolk antibody.

[0018] The "ELISA method" (enzyme-linked immunosorbent assay) is used to detect the ELISA titer of the anti-H3N2 influenza virus-specific chicken egg yolk antibody prepared by the process of the present invention. The test results showed that the titer reached 1:1280.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing a specific egg yolk antibody against influenza A virus H3N2. The method comprises the following steps: producing an influenza A virus H3N2 immune antigen and immune laying hens to obtain immune eggs; extracting a specific egg yolk antibody crude product against influenza A virus H3N2 from the yolks of the immune eggs based on a water dilution method; performing ultrafiltration purification, aseptic filtration and ultralow temperature spray drying on the crude product to obtain the specific egg yolk antibody against influenza A virus H3N2; detecting the purity and activity of the antibody. The method disclosed by the invention has the advantages of low cost and high yield, and the antibody obtained according to the method has high specificity, and can realize industrialized production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a method for preparing an anti-type A H3N2 influenza virus-specific chicken egg yolk antibody. Background technique [0002] Influenza A virus can be divided into 16 subtypes (H1-H16) according to the difference of the hemagglutinin (HA) antigen on its surface, and 9 subtypes (N1-H16) according to the difference of the neuraminidase (NA) antigen. -N9). Influenza viruses produce a new influenza virus (antigenic shift) through the cumulative change of surface protein antigenicity (antigenic drift) or gene rearrangement of different influenza viruses (antigenic shift) to evade the immune recognition of the infected host, thereby spreading and multiplying in the infected population . Influenza A (H3N2) is a respiratory disease caused by the influenza A (H3N2) virus. The H3N2 influenza outbreak in Hong Kong in 1968 killed millions of people. In early 2013, H3N2 seasonal influenz...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/02
Inventor 卢晓露陈文彪
Owner 广东京豪生物制药有限公司
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com