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Three-dimensional tissue and organ culture model and its high-throughput automatic three-dimensional image analysis platform and its application

A technology of tissues and organs and three-dimensional images, applied in the field of bioengineering, can solve problems affecting drug treatment and research and development of new drug sensitivity or reactivity, etc., to achieve the effect of increasing the cure rate and quality of life, optimizing the growth conditions of culture, and having a long cycle

Active Publication Date: 2016-11-23
NANJING KDRB BIOTECH INC LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing technology still lacks an automated platform compatible with high-throughput 3D model cultivation and quantification, which affects the prediction of the sensitivity or responsiveness of various types of tumor tissues to drug treatment and the development of new drugs, and cannot be used clinically and new drugs. Large Sample Operations for Screening

Method used

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  • Three-dimensional tissue and organ culture model and its high-throughput automatic three-dimensional image analysis platform and its application
  • Three-dimensional tissue and organ culture model and its high-throughput automatic three-dimensional image analysis platform and its application
  • Three-dimensional tissue and organ culture model and its high-throughput automatic three-dimensional image analysis platform and its application

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Experimental program
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Embodiment 1

[0040] like figure 1 As shown, a three-dimensional tissue and organ culture model and its high-throughput stereoscopic image analysis platform include a three-dimensional tissue and organ culture system, a three-dimensional scanning sampling system connected to the three-dimensional tissue and organ culture system and a high-capacity Data storage and computer analysis systems. The three-dimensional tissue and organ culture system includes an incubator, in which a porous culture plate is arranged, and a tissue and organ culture support composed of matrigel and collagen is arranged on the culture plate.

[0041] like figure 2 As shown, the three-dimensional scanning sampling system includes an inverted fluorescence microscope and a three-dimensional automatic scanning photography device, and the three-dimensional scanning sampling system includes a phase-contrast lighting source 1 and a fully automatic X-scanning mobile platform 2 located below the phase-contrast lighting sour...

Embodiment 2

[0044] Now take the clinical anti-tumor drug screening as an example to illustrate the working principle and operation steps of the platform of the present invention. There are at least a dozen chemotherapy drugs currently in use, and 6 or 7 targeted therapy drugs. High-throughput screening of multiple patients, multiple samples, and multiple drugs is an urgent need for many large general hospitals and cancer hospitals. If the efficacy information of the drug can be obtained in a short period of time, it will greatly contribute to the improvement of the efficacy of tumor treatment.

[0045] The three-dimensional tissue and organ culture model of the present invention and its high-throughput three-dimensional image analysis platform screening method for anti-tumor drugs, which includes the following steps: image 3 Shown:

[0046] (1) Fully mix Matrigel and collagen according to the optimized ratio, and then inject it into the wells of the culture plate;

[0047] (2) Cut the...

Embodiment 3

[0055] Taking the research of new anti-tumor drugs as an example, the working principle and operation steps of this platform are explained. Finding and screening new drugs, especially targeting specific gene products or pathways, is a key topic in the field of cancer drug development. The present invention can be applied to medicines in pre-clinical and clinical trial phases.

[0056] A panel of tumor cells or tissues is selected, either from an existing cell line or from a clinical tumor patient. Culture on the surface of Matrigel and collagen for 1 week; add different concentrations of new anti-tumor drugs, continue to culture for a week, and perform 3D volume scanning measurement on the tissue every day; finally, quantitatively evaluate the tumor response or the efficacy of new drugs. For effective drugs, gene mutation research of relevant tumor cells can be carried out, so this method can be used for the development of new tumor biomarkers, linking gene mutations and phar...

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Abstract

The invention discloses a three dimensional tissue and organ culture model, a high throughput automatic stereo image analyzing platform thereof, and a method utilizing the provided platform to screen antitumor drugs. The platform can be applied to the culture system of three dimensional tissue and organ / organ-like substance, three dimensional scanning and sampling system, and high-volume data storage and computer analysis system. The provided platform can simultaneously process a large amount of clinical samples and screen a plurality of antitumor drugs, is capable of greatly reducing the cost and maximally reducing the detection time, thus is widely applied to the drug selection schemes and dosage optimization in clinic, novel drug development, and basic researches on interactions among tissue, organ, biological macromolecules, and other micromolecules.

Description

technical field [0001] The invention relates to a high-throughput analysis platform in the field of bioengineering technology, in particular to a three-dimensional tissue and organ (including organoid) culture model and a high-throughput automatic three-dimensional image analysis platform, which can be widely used in individualized clinical Drug screening, new drug development and biological research. Background technique [0002] Chemotherapy is an important treatment for tumors, but there are great individual differences in the clinical response of cancer patients to anticancer therapies, such as chemotherapy, which is manifested in the fact that anticancer drugs are often only effective for some patients, and the reason is considered to be due to genetic differences And driven by the microenvironment formed between the body and the tumor. Many patients suffer from unnecessary and damaging treatments because the sensitivity and resistance of individual tumors to drugs can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12Q1/06
CPCC12M21/08C12M31/10C12Q1/06
Inventor 杭渤毛建华金键李斌
Owner NANJING KDRB BIOTECH INC LTD