Application of phenyl ether compound with antitumor activity

Abstract

The invention discloses an application of a phenyl ether compound as shown in general formula I in preparation of antitumor drugs. The compound of the general formula I has very good antitumor activity, and especially has excellent activity for human bladder cancer LNCap, T24, J82 and human prostate cancer PC-3.

Description

Technical field

[0001] The invention belongs to the field of medicine and relates to the field of anti-tumor drugs. Specifically related to the application of phenyl ether compounds as anti-tumor drugs. Background technique

[0002] The compound of the present invention has been disclosed in patents CN1887847A, CN101119961A, WO2007000098A, US7947734A, KR100963911A, and JP2008546815A. It has bactericidal and acaricidal activities, but there is no report on its application as a preparation of anti-tumor drugs. Contents of the invention

[0003] The object of the present invention is to provide the application of phenyl ether compounds having a structure represented by general formula I in the preparation of anti-tumor drugs.

[0004] The technical solution of the present invention is as follows:

[0005] Application of benzene ether compounds as anti-tumor drugs, such as fluoxastrobin, enoxastrobin, azoxystrobin, bifujunzhi, picoxystrobin ), pyraclostrobin, triclopyricarb...

Examples

Embodiment 1

[0071] Example 1: For the growth inhibition rate of human bladder cancer cells J82, LNCap, T24, and prostate cancer cell PC-3, about 1000 to 3000 cells were planted in a 24-well plate using in vitro cell culture technology, and then each well was Add 1 ml of cell culture medium well known to those skilled in the art that can cultivate the tumor cell lines used in the test, and place in a cell culture incubator (CO 2 5%, 370°C) for 24 hours, then add an appropriate concentration of the above-mentioned substance to be tested into the wells, and note that the volume of the added solution does not exceed 0.5% of the total volume. Let the cells continue to grow in the cell culture incubator. After one week, aspirate the cell culture medium and wash once with cold 1 ml PBS. Then, fix with 1% formalin at room temperature for 10 minutes, and wash once with cold 1 ml PBS. Add 0.1% crystal violet to stain for 30 minutes. Crystal violet recycling. The stained cells were washed slowly ...

Embodiment 2

[0079] Example 2: For the growth inhibition rate of human leukemia HL-60 cells, the conventional MTT method was used to measure the inhibition rate of the test samples on the growth of each human cancer cell. The cells were taken out from the incubator, washed twice with PBS solution, digested with 0.25% trypsin solution, and a medium well-known to those skilled in the art capable of cultivating tumor cell lines for testing was added to stop the digestion of each cell line, and after centrifugation, Pipette to form a cell suspension and count under an inverted microscope. And use the medium for cultivating each cancer cell line to prepare the cells to a concentration of 5x10 4 / ml of cell suspension, add 100 μl of cells to each well of a 96-well plate, place it in 5% carbon dioxide, and incubate overnight in humidified air at 37°C, add the above-mentioned drugs to be tested diluted into different concentration gradients, and let it act for 48 hours. MTT was added, and after 4 ...

Technical field

[0001] The invention belongs to the field of medicine and relates to the field of anti-tumor drugs. Specifically related to the application of phenyl ether compounds as anti-tumor drugs. Background technique

[0002] The compound of the present invention has been disclosed in patents CN1887847A, CN101119961A, WO2007000098A, US7947734A, KR100963911A, and JP2008546815A. It has bactericidal and acaricidal activities, but there is no report on its application as a preparation of anti-tumor drugs. Contents of the invention

[0003] The object of the present invention is to provide the application of phenyl ether compounds having a structure represented by general formula I in the preparation of anti-tumor drugs.

[0004] The technical solution of the present invention is as follows:

[0005] Application of benzene ether compounds as anti-tumor drugs, such as fluoxastrobin, enoxastrobin, azoxystrobin, bifujunzhi, picoxystrobin ), pyraclostrobin, triclopyricarb...

Examples

Embodiment 1

[0071] Example 1: For the growth inhibition rate of human bladder cancer cells J82, LNCap, T24, and prostate cancer cell PC-3, about 1000 to 3000 cells were planted in a 24-well plate using in vitro cell culture technology, and then each well was Add 1 ml of cell culture medium well known to those skilled in the art that can cultivate the tumor cell lines used in the test, and place in a cell culture incubator (CO 2 5%, 370°C) for 24 hours, then add an appropriate concentration of the above-mentioned substance to be tested into the wells, and note that the volume of the added solution does not exceed 0.5% of the total volume. Let the cells continue to grow in the cell culture incubator. After one week, aspirate the cell culture medium and wash once with cold 1 ml PBS. Then, fix with 1% formalin at room temperature for 10 minutes, and wash once with cold 1 ml PBS. Add 0.1% crystal violet to stain for 30 minutes. Crystal violet recycling. The stained cells were washed slowly ...

Embodiment 2

[0079] Example 2: For the growth inhibition rate of human leukemia HL-60 cells, the conventional MTT method was used to measure the inhibition rate of the test samples on the growth of each human cancer cell. The cells were taken out from the incubator, washed twice with PBS solution, digested with 0.25% trypsin solution, and a medium well-known to those skilled in the art capable of cultivating tumor cell lines for testing was added to stop the digestion of each cell line, and after centrifugation, Pipette to form a cell suspension and count under an inverted microscope. And use the medium for cultivating each cancer cell line to prepare the cells to a concentration of 5x10 4 / ml of cell suspension, add 100 μl of cells to each well of a 96-well plate, place it in 5% carbon dioxide, and incubate overnight in humidified air at 37°C, add the above-mentioned drugs to be tested diluted into different concentration gradients, and let it act for 48 hours. MTT was added, and after 4 ...

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