Fingerprint spectrum consisting of small RNAs and application thereof to diagnosis of bladder cancer

A technology of bladder cancer and collection, applied in the fields of biomedicine, bioengineering and detection, can solve the problems of low sensitivity, inability to diagnose patients' compliance in early stage, etc.

Pending Publication Date: 2018-11-02
SHANGHAI XIANGQIONG TECHNOLOGY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although a series of urine biomarkers have been reported in recent years that may be helpful for the early diagnosis, prognosis, recurrence and treatment of bladder cancer, the current gold standard for clinical diagnosis is urine exf

Method used

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  • Fingerprint spectrum consisting of small RNAs and application thereof to diagnosis of bladder cancer
  • Fingerprint spectrum consisting of small RNAs and application thereof to diagnosis of bladder cancer
  • Fingerprint spectrum consisting of small RNAs and application thereof to diagnosis of bladder cancer

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preparation example Construction

[0109] The preparation of the miRNA chip can adopt conventional manufacturing methods of biochips known in the art. For example, if the solid phase carrier is a modified glass slide or silicon wafer, and the 5' end of the probe contains amino-modified poly dT strings, the oligonucleotide probe can be formulated into a solution, and then spotted with a spotting instrument. The miRNA chip of the present invention can be obtained by arranging in a predetermined sequence or array on a modified glass slide or a silicon chip, and then fixing it by standing overnight. If the nucleic acid does not contain amino modification, its preparation method can also refer to: "Gene Diagnosis Technology-Non-Radioactive Operation Manual" edited by Wang Shenwu; J.L.erisi, V.R.Iyer, P.O.BROWN. Exploring the metabolic and genetic control of gene expression on a genomic scale. Science, 1997; 278:680 and Ma Liren, edited by Jiang Zhonghua. Biochip. Beijing: Chemical Industry Press, 2000, 1-130.

[01...

Embodiment 1

[0132] Sample collection and information analysis

[0133] The samples in this study were frozen tissue samples collected from Zhongshan Hospital Affiliated to Fudan University between March 2016 and November 2016. All samples were collected with personal consent and approved by the hospital's ethics committee, and pathological diagnosis results were confirmed by more than two pathologists. A total of 59 samples, including 30 samples of bladder cancer, 29 samples of paracancerous (normal) tissue, aged from 46 to 90 years (average 71.1 years). Samples were removed from patients and stored at -80°C. The clinical data of the patients, including the age of onset, gender, tumor stage, tumor grade and other sample information, are shown in Table 1.

[0134] Table 1 Sample information

[0135]

[0136]

[0137] During the biostatistical analysis, the 59 samples were divided into two parts. The first part was training modeling samples, including 19 bladder cancer samples and ...

Embodiment 2

[0139] Total RNA Extraction

[0140] About 50 mg of tissue fragments were excised from frozen tissues, and total RNA was extracted according to the instructions of miRNeasy mini kit (Qiagen, 217004).

[0141] The quality was detected by electrophoresis, the OD260nm and OD280nm were measured by ultraviolet spectrophotometry, and the RNA concentration was calculated. Store at -80°C.

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Abstract

The invention relates to a fingerprint spectrum consisting of small RNAs and application thereof to diagnosis of bladder cancer. The fingerprint spectrum consisting of a plurality of RNAs can very effectively distinguish a bladder cancer tissue and a paracancerous tissue with high sensitivity and strong specificity, and can be effectively applied to the diagnosis of the bladder cancer.

Description

technical field [0001] The invention relates to the technical fields of biomedicine, bioengineering and detection, in particular to a group of small RNA fingerprints and its application in the diagnosis of human bladder cancer. Background technique [0002] Bladder cancer (Carcinoma of Bladder; Bladder cancer) is a common malignant tumor of the urinary system, the incidence is mainly male, and its incidence rate ranks fourth in the incidence of male tumors, second only to prostate cancer; the mortality rate ranks first among male tumors. The 9th place in men's disease is 14 times that of women's. In China, bladder cancer ranks first among malignant tumors of the male genitourinary system, and has been increasing year by year in recent years. [0003] The vast majority of primary bladder tumors come from epithelial tissue, of which about 90%-95% are urothelial cell carcinoma (UCC), squamous cell carcinoma (3%-7%) and adenocarcinoma (2% )Relatively small. Although a series ...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/11C12N15/63C40B40/06
CPCC12N15/113C12N15/63C12Q1/6886C40B40/06C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 谭若颖张奉武朱苗骏姜帅郭剑明
Owner SHANGHAI XIANGQIONG TECHNOLOGY LTD
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