Method for extracting chlorogenic acid in artemisia scoparia
An extraction method and technology of chlorogenic acid, applied in the separation/purification of carboxylic acid esters, organic chemistry and other directions, can solve the problems of low impurity content, small difficulty in subsequent processes, and great difficulty in subsequent processes, and achieve low impurity content and subsequent processing. The effect of less difficult craftsmanship
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example 1
[0020] Place the fresh bacterial culture in a constant temperature oven at 40°C for 1 hour, crush and sieve it into 40 meshes with a small pulverizer; accurately weigh 10g of the bacterial culture and place it in a triangular flask, add 80mL of distilled water in a water bath at 80°C After 15 minutes, add 80mL of ice water to it, and when the temperature is cooled to 40°C, shake and extract at a speed of 60rpm for 1.5h, squeeze and filter through four layers of gauze, and then centrifuge to obtain the crude extract of chlorogenic acid; Add 100mL of 80% ethanol to the mixture, place it on a water bath and heat it to 80°C and reflux for 1h, and when it is cooled to 30°C, filter it with 40g of 100-mesh diatomaceous earth; place the filtrate in a rotary evaporator and evaporate to dryness, and the residue is washed with 80% ethanol Dissolve the solution into a 25mL brown volumetric flask, add 80% ethanol to the mark, shake well, filter, and take the filtrate; extract twice with 150...
example 2
[0022] Place the fresh bacterial culture in a constant temperature oven at 40°C for 1 hour, crush and sieve it into 45 meshes with a small pulverizer; accurately weigh 10g of the bacterial culture and place it in a triangular flask, add 80mL of distilled water to it in a water bath at 80°C After 15 minutes, add 80mL of ice water to it, and when the temperature is cooled to 40°C, shake and extract at a speed of 60rpm for 1.5h, squeeze and filter through four layers of gauze, and then centrifuge to obtain the crude extract of chlorogenic acid; Add 100mL of 80% ethanol to the mixture, place it on a water bath and heat it to 80-90°C and reflux for 1h, and when it is cooled to 35°C, filter it with 40g of 100-mesh diatomaceous earth; place the filtrate in a rotary evaporator and evaporate to dryness, and use 80 Dissolve % ethanol solution into a 25mL brown volumetric flask, add 80% ethanol to the mark, shake well, filter, and take the filtrate; extract twice with 150mL of dichloromet...
example 3
[0024] Place the fresh bacterial culture in a constant temperature oven at 40°C for 1 hour, crush and sieve it into 50 meshes with a small pulverizer; accurately weigh 10g of the bacterial culture and place it in a triangular flask, add 80mL of distilled water to it in a water bath at 80°C After 15 minutes, add 80mL of ice water to it, and when the temperature is cooled to 40°C, shake and extract at a speed of 60rpm for 1.5h, squeeze and filter through four layers of gauze, and then centrifuge to obtain the crude extract of chlorogenic acid; Add 100mL of 80% ethanol to the solution, put it on a water bath and heat it to 90°C and reflux for 1h, and when it is cooled to 40°C, filter it with 40g of 100-mesh diatomaceous earth; place the filtrate in a rotary evaporator and evaporate the residue to Dissolve the solution into a 25mL brown volumetric flask, add 80% ethanol to the mark, shake well, filter, and take the filtrate; extract 3 times with 150mL dichloromethane, then purify w...
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