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Method for inducing cardiac differentiation of stem cells by utilizing small molecule compounds

A small molecule compound and compound technology, applied in the field of stem cell biology and regenerative medicine, can solve the problems of high price of growth factors and cytokines, unsuitable for large-scale clinical use, carcinogenicity, etc., and achieve potential clinical application value and good safety. , the effect of high induction efficiency

Active Publication Date: 2015-03-25
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although people can induce the myocardial differentiation of stem cells through factors or small molecules, these methods have their limitations, such as DMSO is carcinogenic; growth factors and cytokines are relatively expensive, and are not suitable for large-scale clinical use.

Method used

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  • Method for inducing cardiac differentiation of stem cells by utilizing small molecule compounds
  • Method for inducing cardiac differentiation of stem cells by utilizing small molecule compounds
  • Method for inducing cardiac differentiation of stem cells by utilizing small molecule compounds

Examples

Experimental program
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Effect test

Embodiment 1

[0060] 3-(4'-Benyloxycarbonylamino-biphenyl-4-yl)-propionic acid (E134)

[0061]

[0062] Dissolve 0.5mmol raw material 3-(4'-amino-biphenyl-4-yl)-propionic acid 122mg in 2ml THF and 2ml H 2 O mixed solution, left to cool at 0 °C. At this temperature, dropwise add 0.6mmol raw material Bian oxyacyl chloride 102mg and 1mlNa 2 CO 3 / H 2 O, gradually raised to room temperature, the reaction 5h. The reaction product is saturated with NH 4 Cl solution was dissolved, extracted with ethyl acetate, dried with anhydrous sodium sulfate after recovering the solvent, the extract was subjected to silica gel column chromatography, and 154 mg of white solid product I was obtained with petroleum ether: acetone (6:1-3:1) (Yield 82%). 13 C NMR (125MHz, DMSO-d 6 ( overlap, 2C), 126.6 (overlap, 2C), 119.0, 66.3 (-CH 2 O), 35.6 (-CH 2 ), 30.4 (-CH 2 )( figure 2 ). 1 H NMR (500MHz, DMSO-d 6 ): δ12.14(s, 1H), 9.86(s, 1H), 7.56(m, 6H), 7.42(m, 5H), 7.29(m, 2H), 5.18(s, 2H), 2.85(t, ...

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Abstract

The invention discloses a method for inducing cardiac differentiation of stem cells by utilizing small molecule compounds, relating to the fields of stem cell biology and regenerative medicines. The small molecule compounds refer to a type of carbamate compounds (I) or medicinal salts, hydrates, solvates and isomers thereof. The method comprises the following steps: adding the small molecule compounds into a differential medium to directly act on tested cells, continuously using the small molecule compounds according to the form and growth states of the tested cells and the identification conditions until the expression level of corresponding target genes in the tested cells is increased, and differentiating the tested cells into cardiomyocyte-like cells, wherein the stem cells comprise embryonic stem cells, induced pluripotent stem cells and mesenchymal stem cells. The experiment proves that after the inducers are added into the cell differentiation medium, the myocardial cell differentiation proportion of the stem cells is obviously improved. The differential medium containing a type of carbamate compounds or medicinal salts, hydrates, solvates and isomers thereof can be used for inducing the stem cells to be differentiated into myocardial cells.

Description

technical field [0001] The present invention relates to the field of stem cell biology and regenerative medicine, in particular to the use of small molecular compounds (a class of carbamate compounds or their pharmaceutically acceptable salts, hydrates, solvates and isomers) to induce stem cells to differentiate into myocardial Methods. Background technique [0002] Stem cell-based regenerative medicine has opened up a new and broad field for the development of life science and medicine. Researchers believe that stem cell (regenerative medicine) research has the potential to change the way humans respond to disease by repairing specific tissues or organs. Stem cell regenerative medicine has broad application prospects in the treatment of cardiomyopathy and other diseases that currently have no good treatment options. [0003] Stem cells are primitive undifferentiated cells that have the ability to differentiate into other cell types. According to different sources, stem c...

Claims

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Application Information

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IPC IPC(8): C07C271/28C07C269/04C07D295/192C07D263/26C12N5/077
Inventor 徐秀琴邱彦刘靖林瑜
Owner XIAMEN UNIV
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