Illuminating system of imaging flow cytometry

Abstract

The invention discloses an illuminating system of an imaging flow cytometry and belongs to the technical field of optical design. The system aims to solve the problems of the prior art that optical energy loss is high, utilization rate is low, researching and manufacturing cost is high, stability is poor, and the design of a beam-focusing machine is limited to a certain extent. The system comprises a laser light source, a digital micro mirror array, a piezoelectric ceramics tilt platform, a laser expanding system, a cylindrical mirror and a focusing lens, wherein basic mode light rays output by the laser light source become reflection light rays through the digital micro mirror array, the reflection light rays are expanded through the laser expanding system to form round spot expanded light rays, the expanded light rays are imaged to be oval light spots through the cylindrical mirror, the oval light spots become focused light rays through the focusing lens, the focused light rays are focused into a uniform linear light spot at the position of a target surface, and the digital micro mirror array is arranged on the piezoelectric ceramics tilt platform and achieves high-speed scanning under the control of the piezoelectric ceramics tilt platform.

Description

technical field [0001] The invention belongs to the technical field of optical design, and in particular relates to an illumination system for detection equipment such as an imaging flow cytometer. Background technique [0002] During the development of imaging flow cytometer, high brightness and uniform requirements are put forward for its illumination system. The fundamental mode laser light source (TEM00) with outstanding characteristics such as high brightness, monochromaticity, and coherence has become the most suitable choice. In order to uniformly irradiate the cells and improve the resolution, the diameter of the laser spot irradiated on the cells should be similar to the diameter of the cells. Therefore, the intensity distribution of the laser light used for illumination is required to be flat-topped like a rectangular pulse. [0003] In order to form a flat-top light intensity distribution on the target surface, the illumination system in the current imaging flow...

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Problems solved by technology

[0004] The purpose of the present invention is to propose a lighting system for imaging flow cytometers, which solves the problems of large loss of light energy, low utilization rate, high development cost, poor stability and certain restrictions on the design of light machines in the prior art.

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