Method for separating and purifying high-purity general flavone in kandelia candel leaves

A technology for separating and purifying, and applying to the field of preparation of high-purity total flavonoids from A. chinensis, can solve the problems of low purity, low purity of total flavonoids, environmental pollution and the like, and achieves high pertinence, strong whitening and freckle-removing activity, and simple method. Effect

Inactive Publication Date: 2015-04-01
ZHEJIANG SHUREN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main shortcoming of above-mentioned two kinds of methods is: the obtained total flavonoids purity is low, after passing through macroporous resin, purity is all less than 20%, even after using a large amount of poisonous organic s

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Extraction: take candela leaves and crush them into coarse powder, take 1000g, use ethanol with a volume fraction of 60% as the extractant, the ratio of solid to liquid is 1: 15 (mass to volume ratio), the extraction temperature is 80 °C, and the extraction time is 0.5 h. Extract 2 times;

[0038] (2) Separation and purification: Purify the total flavonoids of candela leaves obtained in step (1) with HPD722 macroporous adsorption resin. The specific purification process is: the concentration of total flavonoids in the sample is 5.0 mg / mL, and the flow rate of the sample is 3BV / h. The sample loading volume is 6.5 BV resin column volume, the adsorption time is 30 min, the impurity is removed by washing with 3.5 times water, the eluent is ethanol with a volume fraction of 50%, the elution flow rate is 3BV / h, and the eluent dosage is 4 BV resin column volume, collected the ethanol solution, recovered the solvent under reduced pressure, dried, and pulverized to obtain 94...

Embodiment 2

[0040] (1) Extraction: take candela leaves and crush them into coarse powder, take 1000g, use ethanol with a volume fraction of 70% as the extractant, the ratio of solid to liquid is 1: 15 (mass to volume ratio), the extraction temperature is 60 °C, and the extraction time is 1.5 h. Extract 2 times;

[0041] (2) Separation and purification: Purify the total flavonoids of candela leaves obtained in step (1) with HPD722 macroporous adsorption resin. The specific purification process is: the concentration of total flavonoids in the sample is 5.0mg / mL, and the flow rate of the sample is 2.5BV / h , the loading volume is 6BV resin column volume, the adsorption time is 30 min, wash with 3.5 times water to remove impurities, the eluent is ethanol with a volume fraction of 35%, the elution flow rate is 4BV / h, and the amount of eluent is 4 BV resin column volume, collect the ethanol liquid, recover the solvent under reduced pressure, dry, and pulverize to obtain 93.23 g of total flavonoi...

Embodiment 3

[0043] (1) Extraction: Take candela leaves and crush them into coarse powder, take 1000g, use ethanol with a volume fraction of 70% as the extractant, the ratio of solid to liquid is 1: 15 (mass to volume ratio), the extraction temperature is 80 °C, and the extraction time is 0.5 h. Extract 2 times;

[0044] (2) Separation and purification: Purify the total flavonoids of candela leaves obtained in step (1) with HPD100 macroporous resin. The sample loading volume is 5.5 BV resin column volume, the adsorption time is 30 min, the impurities are washed with 3.5 times water, the eluent is ethanol with a volume fraction of 50%, the elution flow rate is 3BV / h, and the eluent dosage is 4 BV resin Column volume, ethanol solution was collected, the solvent was recovered under reduced pressure, dried, and pulverized to obtain 85.51 g of total flavonoid powder of yellow-brown candela leaves, with a total flavonoid content of 65.21%.

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Abstract

The invention relates to a preparation method of high-purity general flavone in kandelia candel leaves. The preparation method disclosed by the invention comprises the following steps of: (1) crushing kandelia candel leaves into coarse powder, extracting by using 15 times of ethanol solution with the volume fraction of 60-70% for two times, wherein the extraction time for each time is 0.5-1.5h; the extraction temperature is 60-80 DEG C, and mixing extracting solution; (2) concentrating the extracting solution obtained in the step (1) till the concentration of the extracting solution is 5 mg/ml, centrifuging to obtain supernate, purifying by using nonpolar macroporous adsorption resin, adsorbing for 30 min, washing by using water to remove impurities, and eluting by using 35-80% of ethanol, wherein the nonpolar macroporous adsorption resin is one or more of HPD722 type, adsorption resin HPD100 type adsorption resin and HPD826 adsorption resin; and (3) collecting eluant obtained in the step (2), decompressing to recycle a solvent, drying, and crushing to obtain the high-purity general flavone. The content of general flavone in kandelia candel leaves is at least 60%; the method disclosed by the invention is simple, convenient, high-efficiency, environmentally friendly and high in pertinence; and thus, high-purity general flavone in kandelia candel leaves having high skin whitening and freckle removing activities can be obtained.

Description

technical field [0001] The invention relates to a preparation method of high-purity total flavonoids of candela leaves, belonging to the technical field of biological separation. Background technique [0002] Kandelia candel (Kandelia candel) is a plant of the genus Candel in the family Mangroveaceae. Candel is the most cold-resistant tree species among mangrove plants in my country. From south to north in the distribution area of ​​mangrove plants in my country, the species of mangrove plants gradually decrease, and only candel is still distributed. Reach the northernmost point 28 o 25'N (Yueqing, Zhejiang). The chemical components in candela mainly include flavonoids, tannins, sterols, fatty acids and polysaccharides. In recent years, many scholars have studied various chemical components in candela, and found some functions with drug development value. For example, scholar Li Baocai reported in the article "Research on Lipid Chemistry and Active Substances of Mangrove Ca...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K127/00
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/55
Inventor 李成平唐岚饶桂维计燕萍徐兴严小平活泼
Owner ZHEJIANG SHUREN UNIV
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