Method for utilizing vitex negundo var ineica scrap to culture hericium erinaceus
A technology of vitex shavings and vitex, applied in the directions of botanical equipment and methods, application, cultivation, etc., can solve problems such as waste of wild vitex, and achieve the effects of reuse, high economic benefits, and abundant resources.
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[0042] Embodiment: Utilize the method for cultivating Hericium erinaceus by Vitex shavings, Hericium erinaceus bacterial classification is market purchase
[0043] Step A. Preparation of Liquid Strains
[0044] First-class liquid culture medium formula: 20% potato, 3% bran, 2% glucose, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 10mg / L, CMC 0.3%, natural pH;
[0045] Secondary liquid culture medium formula: Vitex powder 5%, potato 20%, bran 3%, glucose 2%, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 110mg / L, CMC 0.3%, natural pH; among them, Vitex twigs are crushed and sieved through an 80-mesh sieve to obtain a powder, boiled with potatoes and bran for 30 minutes, filtered to remove slag to obtain an extract.
[0046] Use a 500mL Erlenmeyer flask with a liquid volume of 200mL for the preparation of the first-level liquid strains. After autoclaving at 121°C for 30 minutes, insert commercially available PDA slant strains under aseptic conditions, and the inoculation volume of each bottle i...
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