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Chondrocyte culture medium and chondrocyte culture method

A technology of chondrocytes and culture methods, which is applied in the direction of bone/connective tissue cells, animal cells, vertebrate cells, etc., and can solve problems such as limiting the application of chondrocytes, poor proliferation ability of chondrocytes, and serious dedifferentiation tendency of chondrocytes

Active Publication Date: 2015-04-01
SHAANXI RUISHENG BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the inventors have found that in the process of culturing chondrocytes using this chondrocyte medium, the tendency of chondrocyte dedifferentiation is serious, and the proliferation ability of chondrocytes is poor, which greatly limits the application of chondrocytes.

Method used

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  • Chondrocyte culture medium and chondrocyte culture method
  • Chondrocyte culture medium and chondrocyte culture method
  • Chondrocyte culture medium and chondrocyte culture method

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specific Embodiment approach

[0055] Hereinafter, the present invention will be described in detail with reference to Examples, Comparative Examples, and Experimental Examples of the present invention. These examples are merely examples for specifically illustrating the present invention, and those skilled in the art will understand that the scope of the present invention is not limited by these examples, comparative examples, and test examples.

Embodiment 1

[0061] For convenience, the chondrocyte culture medium of Example 1 is called A formula.

[0062] The preparation of A formula:

[0063] Add sterile ultrapure water to the basal medium dry powder DMEM, dissolve to a constant volume, fully dissolve, filter with a 0.22 micron filter membrane, package, and then add 10% autologous serum and 60 μg / ml Vc to the basal culture medium, and A formula is obtained by combining the factors. A formula is as shown in table 1:

[0064] Table 1

[0065]

Embodiment 2

[0067] For convenience, the chondrocyte culture medium of Example 2 is referred to as B formula.

[0068] Preparation of formula B:

[0069] Add sterile ultrapure water to the basic medium dry powder DF12, dissolve to a constant volume, fully dissolve, filter with a 0.22 micron filter membrane, package, then add 5% autologous serum and 20 μg / ml Vc to the basic medium, and Combination of factors to obtain B formula. B formula is as shown in table 2:

[0070] Table 2

[0071]

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Abstract

The invention relates to a chondrocyte culture medium and a chondrocyte culture method. The chondrocyte culture medium can reduce dedifferentiation of the chondrocyte and improve the multiplication capacity of the chondrocyte. The chondrocyte culture medium provided by the embodiment of the invention comprises a basal culture medium, serum with a concentration of 5-20%, a platelet-derived growth factor with a concentration of 1-20 ng / ml, and beta-mercaptoethanol with a concentration of (1-5)*10<-5> M / L.

Description

Technical field [0001] The present invention relates to the field of chondrocyte culture, and in particular to a chondrocyte culture medium and a chondrocyte culture method. Background technique [0002] Articular cartilage is mainly an avascular tissue composed of cartilage cells and extracellular matrix. It mainly relies on the movement and extrusion of joints to absorb nutrients. Therefore, the self-repair ability of cartilage after injury is relatively weak. Repair and transplantation after injury have always been the focus of the medical community. One of the problems. In recent years, with the development of tissue engineering technology, autologous cartilage transplantation has been used in the clinical treatment of cartilage damage at home and abroad. Clinical research has limited access to patient tissue, and because chondrocytes are terminally differentiated cells with limited in vitro proliferation ability and easy dedifferentiation, the culture and expansion of ...

Claims

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Application Information

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IPC IPC(8): C12N5/077
Inventor 邱彩娥田智泉朱君郭静
Owner SHAANXI RUISHENG BIOTECH
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