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Method of isolating and screening antagonistic bacteria from rubber tree leaves

A technology of separation and screening, tree leaves, applied in the field of separation and screening of antagonistic bacteria, can solve the problems that restrict the sustainable development of agriculture, and achieve the effects of controlling the occurrence of diseases, obvious antagonism, and reducing pollution

Inactive Publication Date: 2015-04-01
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the control of rubber anthracnose in production is still dominated by chemical fungicides, and the problems of bacterial resistance, toxicity and environmental pollution caused by chemical pesticides have become increasingly prominent, which has become a major obstacle restricting the sustainable development of agriculture.

Method used

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  • Method of isolating and screening antagonistic bacteria from rubber tree leaves
  • Method of isolating and screening antagonistic bacteria from rubber tree leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for isolating and screening antagonistic bacteria from rubber tree leaves specifically comprises the following steps:

[0026] 1 Sampling and collecting rubber susceptible leaves in different areas, putting the collected leaves into plastic bags and recording.

[0027] 2 Surface disinfection of materials and separation of antagonistic bacteria

[0028] 2.1 Rinse with clean water and cut off the diseased leaves. Wash off the dust and grit on the leaves with clean water and dry them. Use scissors to cut small leaves with a size of 5 × 5mm at the junction of disease and health for subsequent use;

[0029] 2.2 Disinfection of samples Put the small diseased leaves clipped into a beaker and soak them in mercuric chloride for 30 seconds. During the soaking process, care should be taken to constantly stir the small diseased leaves, then rinse with sterile water three times, dry and set aside;

[0030] 2.3 Bacteria isolation and culture Use sterilized tweezers to pa...

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Abstract

The invention relates to a method of isolating and screening antagonistic bacteria from rubber tree leaves. According to the method, bacteria are isolated from diseased leaves by a conventional sampling isolation method, and a strain of the antagonistic bacteria with an obvious inhibition action on the anthracnose pathogen RC178 of rubber trees is obtained by carrying out confront culture on the bacteria and test bacteria colletotrichum RC178 of the rubber trees. The isolated antagonistic bacteria are usually used as biocontrol bacteria and expected to be prepared into a microbial preparation, and therefore a basic preparation is provided for biocontrol of anthracnose of the rubber trees.

Description

technical field [0001] The invention belongs to the field of biological control bacteria, in particular to a method for separating and screening antagonistic bacteria from rubber tree leaves. Background technique [0002] Rubber tree anthracnose is an important disease that seriously affects latex yield and quality in rubber tree production. The pathogenic bacteria are C.gloeosporioides Penz. and C.acutatum Simmons. fungus is more common, and oxysporum anthracnose is a new disease of rubber trees in my country. Rubber tree anthracnose causes leaf tissue necrosis or a large number of shedding, and in severe cases, it causes a large number of fallen leaves and young shoots to wither, the tree body weakens, shortens the rubber tapping period, delays the tapping time, and significantly reduces the total rubber output. [0003] At present, a series of studies on rubber anthracnose have been carried out at home and abroad, mainly focusing on the epidemic of the disease and the scr...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/125
Inventor 郑肖兰梁艳琼胡加谊吴伟怀习金根郑金龙贺春萍易克贤
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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