Active lactobacillus or probiotics heat and convective drying protective agent and preparation method thereof

A technology of active lactic acid bacteria and convection drying, applied in dairy products, milk preparations, applications, etc., can solve problems such as gaps, and achieve the effect of improving residual activity and reducing bacterial damage.

Active Publication Date: 2015-04-08
NANTONG EAST CONCEPT NEW MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual industrial production such as spray drying, there is still a gap in the effect of relying on milk alone to protect lactic acid bacteria or probiotics compared with low-temperature production technology
Compared with adding maltodextrin, starch and other synergistic protective agents to milk, there are very few research reports on the use of simple methods to improve the protection ability of milk itself against bacterial cells

Method used

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  • Active lactobacillus or probiotics heat and convective drying protective agent and preparation method thereof
  • Active lactobacillus or probiotics heat and convective drying protective agent and preparation method thereof
  • Active lactobacillus or probiotics heat and convective drying protective agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0019] Preparation of protective agent: Add 1.1g calcium chloride (approximately equal to 10mmol / L calcium ion) to 100mL milk, stir magnetically at 25°C for 30min, and heat at 60°C for 15min until the milk protein coagulates. The coagulated milk is homogenized to obtain the required protective agent.

[0020] Test strains: Lactobacillus rhamnosus GG (preservation number ATCC53101, hereinafter referred to as LGG, disclosed in patent No. US 4839281 (A)) and Lactobacillus casei Zhang (preservation number CGMCC 1697, hereinafter referred to as LZ, in patent No. CN 101144062A Disclosure), both strains are commercially available.

[0021] Bacterial suspension preparation: the two strains were activated in MRS medium at 37°C for 12 hours, and then cultured in MRS for 24 hours. Centrifuge 1 mL of the fermentation broth of the two strains at 8000 rpm for 15 min, discard the supernatant to collect the bacterial cells. The bacterial cells of the two strains were washed with physiologic...

specific Embodiment 2

[0028] Specific embodiment 2: add 5.5g calcium chloride (approximately equal to 50mmol / L calcium ion) in 100mL milk, heat 10min under 60 ℃, other operations are the same as specific embodiment 1.

[0029] Table 2. Survival rates of two strains of probiotics after heat treatment and changes in survival rate and moisture content during single droplet convective drying

[0030]

specific Embodiment 3

[0031] Specific Example 3: 0.11 g of calcium chloride (approximately equal to 0.1 mmol / L calcium ion) was added to 100 mL of milk, heated at 60° C. for 30 min, and other operations were the same as in Specific Example 1.

[0032] Table 3. The survival rate of two strains of probiotics after heat treatment and the change of survival rate and water content during single droplet convective drying

[0033]

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PUM

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Abstract

The present invention relates to an active lactobacillus or probiotics heat and convective drying protective agent a and preparation method thereof, the drying protective agent is applied to lactobacillus or probiotics dairy products, according to the method, by increasing of calcium ions in milk, milk protein thermal aggregation is produced, and with help of high pressure, stirring and other manners, milk containing aggregated protein can be homogenized to obtain the final protective agent. The preparation method of the protective agent is simple in operation; compared with the untreated milk, the drying efficiency of the milk added with the protective agent is higher, the operation concentration of the milk or addition of other synergistic protective agent can be avoided, the cost of production can be effectively saved; at the same time, the lactobacillus or probiotics heat protection capacity and convective drying protective capacity of the drying protective agent are increased significantly.

Description

technical field [0001] The present invention relates to the preparation method of the thermal and convective drying protection agent of active lactic acid bacteria or probiotics, and the thermal and convective drying protective agent of a kind of active lactic acid bacteria or probiotics prepared by this method, and this kind of drying protective agent in lactic acid bacteria or Application of probiotics in dairy products. Background technique [0002] With the improvement of people's consumption level in our country, healthy diet and functional food have become more and more popular. As one of the important functional foods, lactic acid bacteria products have received more and more attention, especially the probiotic strains of lactic acid bacteria that have been clinically proven to have a clear "probiotic" function, and their market demand is booming. [0003] However, due to the heat sensitivity of the bacterial cells themselves, the current industrial production of suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/152
Inventor 黄松陈晓东
Owner NANTONG EAST CONCEPT NEW MATERIAL CO LTD
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