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A kind of rapid propagation method of Tongdao wood tissue culture

A technique of tissue culture and removing wood, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of no discovery, and achieve the effect of simple and easy operation and high reproduction coefficient

Inactive Publication Date: 2016-08-17
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no relevant literature has been found to report the tissue culture of Tongtuo wood

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Select the young leaves of Tongtuo wood as explants, cut them into length and width of 0.5-1.5cm, sterilize the surface with 70% volume concentration of alcohol for 30 seconds, and then sterilize with 0.1% mass concentration of mercury chloride for 10 minutes, aseptic After washing with water for 4 times, inoculate into the callus induction medium of MS solid medium + 1.5mg / L NAA + 1.0mg / L 6-BA, and carry out callus induction culture at 22-26°C for 25 days (lighting every day 14 hours, the light intensity is 1700lx); transfer the cultured callus to the cluster bud differentiation medium of MS solid medium + 0.05mg / L NAA + 1.0mg / L 6-BA, at 22-26℃ Cultivate for 25 days (14 hours of light per day, light intensity of 1700lx); inoculate the cultured cluster buds into MS solid medium + 0.05mg / L NAA + 1.5mg / L 6-BA + 0.1mg / L GA 3 Proliferate and grow strong seedlings in the culture medium of 22-26°C for 20 days (lighting 14 hours a day, light intensity 1700lx); cut the strong s...

Embodiment 2

[0020] Select the young stems of Tongtuo wood as explants, cut the young stems into 1-2cm long sections, disinfect the surface with 70% volume concentration of alcohol for 20 seconds, and then sterilize with 0.1% mass concentration of mercury chloride for 12 minutes, aseptic After washing with water for 5 times, inoculate into the callus induction medium of MS solid medium + 2.0mg / L NAA + 1.5mg / L 6-BA, and carry out callus induction culture at 22-26°C for 20 days (lighting every day 14 hours, the light intensity is 2000lx); the cultured callus is transferred to the cluster bud differentiation medium of MS solid medium + 0.1mg / L NAA + 1.5mg / L 6-BA and cultured at 22-26°C 20 days (14 hours of light per day, light intensity of 2000lx); inoculate the cultured cluster buds into MS solid medium + 0.04mg / L NAA + 1.0mg / L 6-BA + 0.05mg / L GA 3 Proliferation and strong seedling culture medium for 15 days at 22-26°C (lighting 14 hours a day, light intensity 2000lx); cut the cultured stron...

Embodiment 3

[0022] Select the sprouts that germinated on the roots of Tongtuo, cut them off from the base of the sprouts with a blade, and use them as explants. After 10 seconds of surface disinfection with 70% volume concentration of alcohol, they were then sterilized with 0.1% mass concentration of mercuric chloride for 5 minutes. Rinse with bacterial water for 3 times and inoculate into the callus induction medium of MS solid medium + 3.0mg / L NAA + 2.0mg / L 6-BA, and carry out callus induction culture at 22-26°C for 30 days (light 14 hours a day, the light intensity is 1400lx); transfer the induced callus to the cluster bud differentiation medium of MS solid medium + 0.20mg / L NAA + 2.0mg / L 6-BA at 22-26℃ Cultivate for 30 days (14 hours of light per day, light intensity of 1400lx); inoculate the cultured cluster buds into MS solid medium + 0.06mg / L NAA + 2.0mg / L 6-BA + 0.5mg / L GA 3 Proliferation and strong seedling culture medium for 25 days at 22-26°C (lighting 14 hours a day, light int...

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Abstract

The invention provides a rice paper plant tissue culture rapid breeding method, and belongs to the technical field of agriculture planting. The method includes the steps of conducting surface disinfection, callus inducement, bud differentiation culture and multiplication, rooting induction, plant exercising and transplantation with rice paper plant young leaves or young stems or young buds as explant. The tissue culture rapid breeding technical system for rice paper plants is established for the first time, the operation method is easy, convenient and feasible, the breeding coefficient is high, 20 times to 25 times of multiplication can be achieved every 60 days, the tissue culture seedling rooting rate reaches 98% or above, the plant exercising and transplanting survival rate reaches 92% or above, and the large-scale production can be conducted.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a tissue culture rapid propagation method of wild medicinal plant Tongtuo. Background technique [0002] Tonto wood ( Tetrapanax papyriferus ) is the genus Araliaceae of Umbelliferae, a deciduous shrub or small tree. The trunk is straight and unbranched, the leaves are mostly lobed, umbels, the stem is thick and shaped, and the pith is white. It is commonly known as "Tongcao", and it is distributed in southern Shaanxi and the south of my country. Tongtuo wood has important garden ornamental and medicinal values, and has the functions of diuresis, heat-clearing and detoxifying, reducing swelling and promoting milk. The pith tissue of the stem can be taken out to make surgical dressings, and it is also the raw material for making rice paper, which can be used as watercolor painting paper. Since it is difficult to germinate into seedlings after sowing, the seeds of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 蔺万煌谭鹏赵彦
Owner HUNAN AGRICULTURAL UNIV
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