A kind of preparation method of tissue repair material
A tissue repair and membrane tissue technology, applied in the field of tissue engineering medical biomaterials, can solve the problems of affecting repair effect and functional recovery, affecting tissue recovery effect, easy shrinkage, etc., to achieve high biosafety and biocompatibility , reducing the risk of organic solvent residues, the effect of small changes in mechanical properties and degradability
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Embodiment 1
[0041] Example 1 、 Preparation of tissue repair materials from porcine peritoneum
[0042] Step 1. Pre-treatment: flatten the pig peritoneum on a flat plate, tear off a large piece of fat, remove the residual fat and connective tissue, wash with water until bloodless, and obtain the pig peritoneum material;
[0043] Step 2. Degreasing: Place the obtained membrane material in a mixed solution of methanol and chloroform (volume ratio 1:2), shake and degrease for 10 hours, then change the liquid, repeat the degreasing 3 times, and wash with water until odorless;
[0044] Step 3. Disinfection: Soak the degreased membrane material in an ethanol solution with a volume concentration of 75% for 3 hours, and wash it with water until it is odorless;
[0045] Step 4, decellularization: place the sterilized membrane material in a decellularized hypertonic solution containing 3M NaCl and 0.25 M NaOH, shake for 30 minutes, then transfer to water and shake for 30 minutes, and repeat this...
Embodiment 2
[0049] Example 2 、 Preparation of tissue repair materials from sheep bladder matrix membrane
[0050] Step 1. Pretreatment: flatten the sheep bladder on a flat plate, separate the matrix membrane, wash with water until bloodless, and obtain the sheep bladder matrix membrane material;
[0051] Step 2, degreasing: place the obtained membrane material in acetone, shake and degrease for 2 hours, then change the liquid, continue shaking and degreasing for 4 hours, then wash with water until odorless;
[0052] Step 3. Disinfection: Soak the degreased membrane material in an ethanol solution with a volume concentration of 75% for 2 hours, and wash it with water until it is odorless;
[0053] Step 4, decellularization: place the sterilized membrane material in a decellularized hypertonic solution containing 1M NaCl and 0.2M HCl, shake for 15 minutes, then transfer to water and shake for 15 minutes, repeat this process 3 times to complete decellularization;
[0054] Step 5, swelli...
Embodiment 3
[0057] Example 3 、 Preparation of tissue repair materials from bovine pericardium
[0058] Step 1, pre-treatment: flatten the bovine pericardium on a flat plate, tear off a large piece of fat on it, remove residual fat and connective tissue, wash with water until bloodless, and obtain the bovine pericardium material;
[0059] Step 2, degreasing: place the obtained membrane material in isopropanol, shake and degrease for 4 hours, and then change the liquid, then repeat the shaking and degreasing twice for 10 hours and 4 hours respectively, and wash with water until odorless;
[0060] Step 3. Disinfection: Soak the degreased membrane material in an ethanol solution with a volume concentration of 70% for 4 hours, and wash it with water until it is odorless;
[0061] Step 4, decellularization: place the sterilized membrane material in a decellularized hypertonic solution containing 5M NaCl and 1M NaOH, shake for 1 hour, then transfer to water and shake for 20 minutes, and repe...
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