Fluorescence PCR detection kit for human K-RAS gene mutation

Abstract

The invention provides a fluorescence PCR detection kit for human K-RAS gene mutation. The kit comprises a forward primer sequence K-M1 PCR reaction solution, a forward primer sequence K-M2 PCR reaction solution, a forward primer sequence K-M3 PCR reaction solution, a forward primer sequence K-M4 PCR reaction solution, a forward primer sequence K-M5 PCR reaction solution, a forward primer sequence K-M6 PCR reaction solution and a forward primer sequence K-M7 PCR reaction solution which are used for detecting Gly12Asp mutation, Gly12Val mutation, Gly12Ser mutation, Gly12Cys mutation, Gly12Ala mutation, Gly12Arg mutation and Gly13Asp mutation on a codon 12 and codon 13. According to the kit, under the 100ng / reaction wild-type human DNA background, amplification signals and the false positive condition are avoided, and it shows that the kit is good in specificity and high in antijamming capability. By the adoption of the kit, human K-RAS gene mutation in samples such as clinical paraffin sections can be detected quickly, and a reliable experiment basis is provided for diagnosing related cancer therapies.

Description

Technical field [0001] The invention provides a fluorescent PCR detection kit for human K-RAS gene mutation. Background technique [0002] Clinically, the targeted drugs Erbitux (Cetuximab) and Panitumumab (Panitumumab) are only suitable for wild-type patients with K-RAS gene without mutation, and are not effective for patients with K-RAS mutation. The National Comprehensive Cancer Network (NCCN) included the K-RAS test in the "Clinical Treatment Guidelines for Colon Cancer." [0003] The K-RAS gene is a recognized oncogene. The results of clinical studies were released at the American Society of Clinical Oncology (ASCO) annual meeting as early as June 2008, that is, patients with K-RAS mutants do not benefit from anti-EGFR treatment, but instead This increases the risk of adverse reactions and the cost of treatment; and K-RAS wild-type patients are likely to benefit from such drug treatment. In October of the same year, the K-RAS gene mutation test was written into the latest ed...

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the defects of the existing human K-RAS gene mutation fluorescence PCR detection kit, and provide a human K-RAS gene mutation fluorescence PCR detection kit with fast operation, simple and convenient method, high detection sensitivity and wide detection range

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