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Application of Sinorhizobium meliloti CCNWSX0020

A rhizobia and heavy metal technology, applied in the field of soil bioremediation, can solve the problems of leghemoglobin function blockage, nitrogenase activity reduction, oxidative stress, etc., to achieve the response promotion of antioxidant enzyme system in plants and increase the absorption of heavy metals Effect

Active Publication Date: 2015-04-29
NORTHWEST A & F UNIV
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Problems solved by technology

It has also been reported that excessive heavy metal stress can seriously inhibit the activities of SOD, CAT, POD and other antioxidant enzymes in root nodules of legumes, which in turn leads to more serious oxidative stress on plant cells, the reduction of nitrogenase activity, and the reduction of nitrogenase activity in legumes. Hemoglobin dysfunction, etc.

Method used

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  • Application of Sinorhizobium meliloti CCNWSX0020

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Embodiment

[0029] Preparation of bacterial suspension and cultivation of plants

[0030] (1) The strains to be tested were inoculated in TY liquid culture medium, shaken at 28°C for 150r.min -1 Shake culture for 24-30h until OD600 is about 0.8, and the bacterial suspension is at 8000r.min -1 Centrifuge for 5 minutes to collect the bacteria, and use sterile ddH 2 o 2 Wash twice and resuspend in nitrogen-free nutrient solution for later use.

[0031] (2) Put 100g of plant planting substrate vermiculite and perlite mixture (V / V=2:1) ​​in a pot (10cm in diameter), spread a layer of gauze on the bottom of the pot to prevent leakage, and then seal it, 121 Sterilize by autoclaving at ℃ for 1 h. After that, a certain volume of Cu 2+ Dilute the stock solution in sterile nitrogen-free nutrient solution, pour it into the sterilized pots, and pour it thoroughly (about 180ml per pot), so that the Cu in the pots 2+ The final concentration of stress was 200 mg / kg. Seal the treated pot again, and...

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Abstract

The invention relates to application of Sinorhizobium meliloti CCNWSX0020, and particularly relates to application of Sinorhizobium meliloti CCNWSX0020 to improving response of antioxidant enzyme systems in plants to heavy metal. Under the excessive heavy metal stress condition, the Sinorhizobium meliloti CCNWSX0020 can be used for improving the biomass, plant height, total N content and heavy metal absorptive amount of plants to different extents. Meanwhile, the inventor finds that the Sinorhizobium meliloti CCNWSX0020 can be used for remarkably promoting the response of the antioxidant enzyme systems in plants under excessive heavy metal stress condition. A symbiotic nitrogen fixation system formed by the Sinorhizobium meliloti CCNWSX0020 and the host plant medicago lupulina has a certain application potential in the bioremediation of heavy metal polluted soil.

Description

technical field [0001] The invention relates to the field of soil bioremediation, in particular to a rhizobium strain Sinorhizobium meliloti CCNWSX0020 resistant to heavy metal copper, and its promotion effect on the growth, copper absorption and antioxidant enzyme system response of the host plant alfalfa, and the symbiotic nitrogen fixation established with it The application potential of the system in the bioremediation of heavy metal contaminated soil. Background technique [0002] Oxygen is one of the important substances in the metabolic activities of plant life. Plants produce O during their own aerobic metabolism 2- , H 2 o 2 , OH and other highly active free radicals. These reactive oxygen species have strong chemical activity and can cause damage to plant cells. In the late 1960s, after the theory of biological free radical (Free Radical) damage was proposed, it has been widely used in the research of aerobic biological toxicity. Under normal circumstances, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P21/00B09C1/10
Inventor 韦革宏孔召玉陈卫民
Owner NORTHWEST A & F UNIV
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