Method for extracting alcohol soluble substance from bark of mongolian pine

A pine bark and alcohol-soluble technology, which is applied in the separation/purification of carboxylic acid compounds, the separation/purification of medical raw materials derived from gymnosperms subphylum, and the separation/purification of carboxylic acid esters can solve the problem of low resource utilization and achieve extraction High efficiency, good separation effect, and reduced difficulty

Active Publication Date: 2015-04-29
INNER MONGOLIA MANZHOULI SENNUO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Pinus sylvestris is one of the main excellent afforestation tree species in the three northern regions of my country. It is widely

Method used

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  • Method for extracting alcohol soluble substance from bark of mongolian pine
  • Method for extracting alcohol soluble substance from bark of mongolian pine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 A kind of method of extracting alcohol-soluble substance from Pinus sylvestris sylvestris bark

[0029] Including the following steps:

[0030] (1) Crush 20g of Pinus sylvestris bark into particles of 20-80 mesh size, put it into 300mL of n-hexane / chloroform, the volume ratio of n-hexane to chloroform is 1:5, and apply ultrasonic waves for 30min at 35°C , Ultrasonic frequency is 25KHz.

[0031] (2) vacuum filter the treated mixture while it is hot to obtain filtrate a and filter residue. The filtrate a was placed in a rotary evaporator for vacuum distillation, and the n-hexane / chloroform solvent was distilled off to obtain lipid substances.

[0032] (3) Put the filter residue in step 2) into 400 mL, 50% ethanol solution, and apply ultrasonic wave at 30° C. for 30 min, and the ultrasonic frequency is 19 KHz.

[0033] (4) vacuum filter the treated mixture while hot to obtain filtrate b. The filtrate a was placed in a rotary evaporator for vacuum distillat...

Embodiment 2

[0036] Embodiment 2 A kind of method of extracting alcohol-soluble substance from Pinus sylvestris sylvestris bark

[0037] (1) Crush 20g of Pinus sylvestris bark into particles of 30-60 mesh size, put it into 400mL of n-hexane / chloroform, the volume ratio of n-hexane and chloroform is 1:1, and apply ultrasonic waves for 60min at 50°C , Ultrasonic frequency is 30KHz.

[0038] (2) vacuum filter the treated mixture while it is hot to obtain filtrate a and filter residue. The filtrate a was placed in a rotary evaporator for vacuum distillation, and the n-hexane / chloroform solvent was distilled off to obtain lipid substances.

[0039] (3) Put the filter residue in step 2) into 500 mL of 80% ethanol solution, and apply ultrasonic wave at 35° C. for 60 min, and the ultrasonic frequency is 25 KHz.

[0040] (4) vacuum filter the treated mixture while hot to obtain filtrate b. The filtrate b was concentrated under reduced pressure with a rotary evaporator, and the ethanol solvent wa...

Embodiment 3

[0043] Embodiment 3 A kind of method of extracting alcohol-soluble substance from Pinus sylvestris sylvestris bark

[0044] (1) Crush 20g of Pinus sylvestris bark into particles of 30-70 mesh size, put it into 350mL of n-hexane / chloroform, the volume ratio of n-hexane and chloroform is 1:2, and apply ultrasonic waves for 50min at 40°C , Ultrasonic frequency is 35KHz.

[0045] (2) vacuum filter the treated mixture while it is hot to obtain filtrate a and filter residue. The filtrate a was placed in a rotary evaporator for vacuum distillation, and the n-hexane / chloroform solvent was distilled off to obtain lipid substances.

[0046] (3) Put the filter residue in step 2) into 500 mL of 70% ethanol solution, and apply ultrasonic wave at 30° C. for 55 min, and the ultrasonic frequency is 25 KHz.

[0047] (4) vacuum filter the treated mixture while hot to obtain filtrate b. The filtrate b was concentrated under reduced pressure with a rotary evaporator, and the ethanol solvent wa...

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Abstract

A method for extracting an alcohol soluble substance from bark of mongolian pine comprises the following steps: (1), smashing the bark of mongolian pine into particles of 20 to 80 meshes, feeding the particles into n-hexane/chloroform and carrying out ultrasonic treatment to obtain a mixture A; (2). filtering the mixture A to obtain filtrate a and residue, and distilling the filtrate a to distil off n-hexane/chloroform to obtain lipid; (3). feeding the residue into an ethanol solution and carrying out ultrasonic treatment; (4). filtering mixture B to obtain filtrate b, distilling the filtrate b to distil off the ethanol solution to obtain the concentrated solution of the alcohol soluble substance, and drying the concentrated solution to obtain the alcohol soluble substance. The alcohol soluble substance extracted by the method is high in content of flavone, contains sinapic acid, secoisolariciresinol, 7-hydroxy-secoisolariciresinol-9'-p-hydroxy(trans) cinnamate and other effective components, and has anti-oxidation and antimicrobial effects and the like.

Description

technical field [0001] The invention belongs to the extraction process of plant effective components, in particular to a method for segmentally extracting natural products from pine bark assisted by ultrasonic waves. Background technique [0002] Pinus sylvestris is one of the main excellent afforestation tree species in the Three North Regions of my country. It is widely distributed and rich in resources. As an important by-product of forestry, the bark of Pinus sylvestris is low in resource utilization. [0003] Studies have shown that the alcohol-soluble substances extracted from Pinus sylvestris sylvestris bark with ethanol contain active ingredients such as polysaccharides, phenolic substances, organic acids, tannins, and flavonoids. Among them, flavonoids are recognized as natural extracts with multiple health benefits. The oil content in Pinus sylvestris is relatively low, about 1%-3% of the bark dry weight. [0004] Because the oil content in the bark of Pinus sylv...

Claims

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Application Information

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IPC IPC(8): C07D311/40C07D311/32C07D311/30C07C59/64C07C51/42C07C43/23C07C41/34C07C69/732C07C67/48A61K36/15
CPCC07C41/34C07C51/42C07C67/48C07D311/30C07D311/32C07D311/40C07D493/20C07C59/64C07C43/23C07C69/732A61K36/15
Inventor 孙昊东孙大庆王桂华
Owner INNER MONGOLIA MANZHOULI SENNUO BIOTECH CO LTD
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