Antioxidant polypeptide with Raja porosa meat protein as well as preparation method and application of antioxidant polypeptide

A technology for antioxidant polypeptides and fish meat protein, which is applied in the preparation methods of peptides, chemical instruments and methods, food preparation, etc., can solve the problem of no reports of highly active antioxidant polypeptides, etc., and achieve the effect of no toxic and side effects.

Inactive Publication Date: 2015-04-29
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the applicant has found that the preparation of anti-oxidative polypeptides using enzymatic hydrolysis technology is still in a blank stage...

Method used

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  • Antioxidant polypeptide with Raja porosa meat protein as well as preparation method and application of antioxidant polypeptide
  • Antioxidant polypeptide with Raja porosa meat protein as well as preparation method and application of antioxidant polypeptide
  • Antioxidant polypeptide with Raja porosa meat protein as well as preparation method and application of antioxidant polypeptide

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Embodiment

[0026] (1) Mince the ray fish meat, add isopropanol according to the solid-liquid ratio of 1 g: 5 mL, degrease at 35 °C for 2 h, centrifuge at 9 000 r / min for 8 min, collect the precipitate, dry it, and obtain the degreased hole Ray meat;

[0027] (2) Add phosphate buffer (0.05 mol / L, pH 7.0) to the defatted ray meat at a solid-to-liquid ratio of 1 g: 6 mL, and add neutral protease (enzyme activity ≥ 1.5 AU / g), hydrolyzed at 60°C for 5 h;

[0028] (3) The enzymatic solution was centrifuged at 10 000 r / min at 4°C for 20 min at low temperature, the precipitate was removed, and the supernatant was taken; the supernatant was subjected to ultrafiltration and chromatography in turn to obtain the antioxidant polypeptide, which was analyzed by amino acid sequence and mass spectrometry to determine its structure, the specific process is:

[0029] ①Ultrafiltration: The supernatant was subjected to ultrafiltration treatment with an ultrafiltration membrane with a molecular weight cut-o...

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Abstract

The invention discloses antioxidant polypeptide with Raja porosa meat protein as well as a preparation method and application of antioxidant polypeptide. The amino acid sequence of the antioxidant polypeptide is Asn-Trp-Asp-Met-Glu-Lys-Ile-Trp-Asp, and ESI/MS detection molecular weight is 1236.38 Da. The preparation method of the antioxidant polypeptide comprises the following steps: tissue mincing, enzymolysis, ultrafiltration, chromatography and the like. The high activity polypeptide Asn-Trp-Asp-Met-Glu-Lys-Ile-Trp-Asp prepared by the preparation method can effectively eliminate hydroxyl radicals, DPPH free radicals and superoxide anion free radicals, and can be used as drugs or health-care foods relevant to oxidation resistance.

Description

technical field [0001] The invention relates to an anti-oxidation polypeptide of ray ray meat protein, and also relates to a preparation method of the anti-oxidation polypeptide of ray ray meat protein. Background technique [0002] Hole Ray ( Raja porosa ) is a fish of the ray family, commonly known as Laobanyu, Laozi, Fuyu, etc. It is distributed in the western North Pacific Ocean, and is produced in the East China Sea, South China Sea, Yellow Sea, and Bohai Sea. The meat of Kong Ray has more thorns and less bones, and its meat can be eaten fresh, but most of it is salted and processed into light-dried fish. Dried ray fish is an aquatic product that coastal residents in Liaoning, Shandong and other provinces are accustomed to eating. [0003] At present, the applicant has found that the preparation of anti-oxidative polypeptides using enzymatic hydrolysis technology is still in a blank stage, and the preparation of high-activity anti-oxidative polypeptides and their a...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/18C07K1/20A61K38/08A61P39/06A23L1/305A23L33/18
Inventor 潘欣王斌迟长凤陈荫
Owner ZHEJIANG OCEAN UNIV
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