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A kind of green thorn fruit extract and its preparation method and application

A technology of green thorn fruit and extract, which is applied in the directions of pharmaceutical formulations, drug combinations, plant raw materials, etc., can solve the problems of non-extraction and anti-aging of green thorn fruit non-oil extracts that have not yet been found.

Active Publication Date: 2021-06-01
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in these two prior art, the former only provides the application of the non-oily extract of the thorn fruit in whitening, while the latter uses propane as the extraction solvent to obtain the non-polar components of the thorn fruit oil , cannot extract the more polar components such as flavonoids
The use and report of the non-oily extract of the green thorn fruit in the field of cosmetics for anti-aging has not yet been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of the thorn fruit extract of the present invention

[0022] 100 mL of 70% ethanol was added to 50 g of commercially available thorn fruit, ultrasonically extracted twice at room temperature for 1 hour each time, and the two extracts were combined to obtain crude ethanol extract of thorn fruit.

[0023] Divide the ethanol crude extract of the green thorn fruit into two parts, take 50ml and directly evaporate to dryness to obtain the green thorn fruit ethanol extract, concentrate the remaining 50ml to 25ml under reduced pressure, adsorb it with an AB-8 resin column, and then sequentially use a deionized Water, 30% ethanol, 70% ethanol and 95% ethanol were eluted, and the solvent was distilled off under reduced pressure after collecting respectively, and correspondingly obtained the water elution part A, 30% ethanol elution part B, 70% ethanol elution part Site C and 95% ethanol elution site D;

[0024] The ethanol extract obtained above and...

Embodiment 2

[0025] Embodiment 2: the preparation of the thorn fruit extract of the present invention

[0026] 100 mL of deionized water was added to 50 g of commercially available thorn fruit, ultrasonically extracted twice at room temperature, each time for 1 hour, and the 2 extracts were combined to obtain a crude water extract of thorn fruit.

[0027] Divide the crude water extract of thorn fruit into two parts, take 50ml and directly evaporate to dryness to obtain the water extract of thorn fruit, concentrate the remaining 50ml to 25ml under reduced pressure, adsorb it with AB-8 resin column, and then successively use deionized Water, 30% ethanol, 70% ethanol and 95% ethanol were eluted, and the solvent was distilled off under reduced pressure after being collected respectively, and the water elution part E, 30% ethanol elution part F, and 70% ethanol elution part were obtained correspondingly. Site G and 95% ethanol elution site H;

[0028] The above-mentioned water extract of thorn...

Embodiment 3

[0029] Embodiment 3: Determination of total flavonoids content in the green thorn fruit extract

[0030] With each of the green thorn fruit extracts of Example 1 and Example 2 as the detection object, according to the method of the Pharmacopoeia of the People's Republic of China (2010 edition), with rutin as the standard substance, the total flavonoid content in the extract was determined. Determination steps are: 1) standard curve making: accurately measure reference substance solution 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, put in 25ml measuring bottle respectively, add 30% ethanol to 6.0ml respectively, add 5% sodium nitrite Solution 1ml. Mix well, let stand for 6 minutes, add 1ml of 10% aluminum nitrate solution, shake well, let stand for 6 minutes. Add 10ml of sodium hydroxide test solution, add 30% ethanol to the scale, shake well, let stand for 15 minutes, use the corresponding reagent as a blank, measure the absorbance at a wavelength of 510nm by UV-visible spectrophotometry, a...

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PUM

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Abstract

The invention relates to the technical field of plant extracts, and discloses a green thorn fruit extract, a preparation method and application thereof. The preparation method of the thorn fruit extract of the present invention adds water or ethanol to the thorn fruit to extract the thorn fruit, and obtains the thorn fruit crude extract; the thorn fruit crude extract is concentrated, loaded onto a macroporous adsorption resin, and deionized water , 30% ethanol solution, 70% ethanol solution and 95% ethanol solution elution, respectively collect each elution position and remove the solvent by distillation under reduced pressure, correspondingly obtain the water elution position, 30% ethanol solution elution position, 70% ethanol solution elution site and 95% ethanol solution elution site. In the present invention, the green thorn fruit is used as the raw material, and the non-oil extract of the green thorn fruit is extracted through a suitable method. The extracted green thorn fruit extract has significant free radical scavenging activity and the effect of promoting the proliferation of human fibroblasts, and can be used In skin anti-aging and related cosmetics.

Description

technical field [0001] The present invention relates to the technical field of plant extracts, in particular to a green thorn fruit extract and its preparation method and application. Background technique [0002] Green thorn fruit is the fruit of Prinsepia utilis Royle (Prinsepia Royle) plant in the Rosaceae family, also known as green thorn point, cannon barrel fruit, milk hammer, chicken cake, plum blossom thorn, gun fruit, Anas, Mengzi flat core wood. Green thorn fruit is mainly distributed in Yunnan, Guizhou, Sichuan and other provinces, and is rich in the pollution-free zone above 2000m above sea level in Lijiang, Yunnan. The fruiting period of the green thorn fruit is from April to May. The ripe fresh green thorn fruit is purple-red, with a smooth surface. The dried fruit is dark brown or black. The peel shrinks and sticks to the core. The core is not easy to break. Rich in oil, the oil yield is about 30%, which can be used for food and daily use. Green thorn fruit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K36/73A61P17/18A61Q19/08
CPCA61K8/97A61K36/73A61K2236/331A61K2236/333A61K2236/55A61Q19/08
Inventor 方兆华沈忠海章漳张佳伟席淑云
Owner JALA GROUP CORPORATION
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