A kind of green thorn fruit extract and its preparation method and application
A technology of green thorn fruit and extract, which is applied in the directions of pharmaceutical formulations, drug combinations, plant raw materials, etc., can solve the problems of non-extraction and anti-aging of green thorn fruit non-oil extracts that have not yet been found.
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Embodiment 1
[0021] Embodiment 1: the preparation of the thorn fruit extract of the present invention
[0022] 100 mL of 70% ethanol was added to 50 g of commercially available thorn fruit, ultrasonically extracted twice at room temperature for 1 hour each time, and the two extracts were combined to obtain crude ethanol extract of thorn fruit.
[0023] Divide the ethanol crude extract of the green thorn fruit into two parts, take 50ml and directly evaporate to dryness to obtain the green thorn fruit ethanol extract, concentrate the remaining 50ml to 25ml under reduced pressure, adsorb it with an AB-8 resin column, and then sequentially use a deionized Water, 30% ethanol, 70% ethanol and 95% ethanol were eluted, and the solvent was distilled off under reduced pressure after collecting respectively, and correspondingly obtained the water elution part A, 30% ethanol elution part B, 70% ethanol elution part Site C and 95% ethanol elution site D;
[0024] The ethanol extract obtained above and...
Embodiment 2
[0025] Embodiment 2: the preparation of the thorn fruit extract of the present invention
[0026] 100 mL of deionized water was added to 50 g of commercially available thorn fruit, ultrasonically extracted twice at room temperature, each time for 1 hour, and the 2 extracts were combined to obtain a crude water extract of thorn fruit.
[0027] Divide the crude water extract of thorn fruit into two parts, take 50ml and directly evaporate to dryness to obtain the water extract of thorn fruit, concentrate the remaining 50ml to 25ml under reduced pressure, adsorb it with AB-8 resin column, and then successively use deionized Water, 30% ethanol, 70% ethanol and 95% ethanol were eluted, and the solvent was distilled off under reduced pressure after being collected respectively, and the water elution part E, 30% ethanol elution part F, and 70% ethanol elution part were obtained correspondingly. Site G and 95% ethanol elution site H;
[0028] The above-mentioned water extract of thorn...
Embodiment 3
[0029] Embodiment 3: Determination of total flavonoids content in the green thorn fruit extract
[0030] With each of the green thorn fruit extracts of Example 1 and Example 2 as the detection object, according to the method of the Pharmacopoeia of the People's Republic of China (2010 edition), with rutin as the standard substance, the total flavonoid content in the extract was determined. Determination steps are: 1) standard curve making: accurately measure reference substance solution 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, put in 25ml measuring bottle respectively, add 30% ethanol to 6.0ml respectively, add 5% sodium nitrite Solution 1ml. Mix well, let stand for 6 minutes, add 1ml of 10% aluminum nitrate solution, shake well, let stand for 6 minutes. Add 10ml of sodium hydroxide test solution, add 30% ethanol to the scale, shake well, let stand for 15 minutes, use the corresponding reagent as a blank, measure the absorbance at a wavelength of 510nm by UV-visible spectrophotometry, a...
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