Method for producing ethanol by mixing bacteria in the presence of inhibitors

Abstract

The invention relates to the technical field of biomass new energy sources and discloses a method of producing ethanol from mixed bacterial by virtue of fermentation in the presence of an inhibitor. The method comprises the following steps: (1) preprocessing straws to obtain a solid-liquid mixture, and separating to obtain the preprocessed substrate; (2) adding cellulase and water to the substrate, regulating the pH and performing enzymatic hydrolysis; (3) inoculating the mixed solution obtained in the step (2) with saccharomyces cerevisiae collected with the number of CGMCC No.2757 for primary fermentation, next, inoculating with saccharomyces cerevisiae collected with the number of CGMCC No. 6634 for secondary fermentation, and performing solid-liquid separation after the fermentation is completed, thereby obtaining a fermentation liquid rich in ethanol. According to the method, the preprocessed straws can be applied to the next step of fermentation process without any detoxification process, the utilization of xylose is obviously improved in the presence of the inhibitor, and the co-utilization of the glucose and the xylose is realized, and the yield of the ethanol is increased.

Description

technical field [0001] The invention relates to the technical field of biomass new energy, in particular to a method for producing ethanol by mixing bacteria in the presence of an inhibitor, and in particular to a method for producing ethanol by improving the utilization of xylose and realizing the co-utilization of glucose and xylose in the presence of an inhibitor . Background technique [0002] At present, the proven reserves of crude oil on the earth have not increased and the output is close to the limit. Human beings are facing the severe challenge of energy and oil crisis. Many countries have begun to formulate or adjust energy policies, and put the research and development of bioenergy in an important position. In renewable energy, ethanol has attracted much attention as a safe and clean fuel and gasoline additive. The greenhouse gas CO can be kept in the whole cycle of ethanol production and use 2 balance. The traditional ethanol fermentation industry mainly use...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that a large amount of inhibitors produced in the existing pretreatment process will seriously affect the fermentation effect of the fermentation strain, and the xylose utilization pathway is more likely to be affected as a pathway obtained by gene recombination in the presence of inhibitors. A method for fermenting and producing ethanol in the presence of inhibitors, which allows the pretreated stalks to enter the next fermentation process without any detoxification process, and can significantly improve the utilization of xylose, and realize glucose and xylose co-utilization of sugar