Method for producing medium-chain-length polyhydroxyalkanoates by virtue of excess activated sludge

A technology of polyhydroxyalkanoate and residual activity, which is applied in the direction of fermentation, etc., to achieve the effect of large application prospects, easy operation and simple equipment

Inactive Publication Date: 2015-04-29
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for producing medium-chain-length polyhydroxyalkanoates by virtue of excess activated sludge
  • Method for producing medium-chain-length polyhydroxyalkanoates by virtue of excess activated sludge

Examples

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Embodiment 1

[0019] Example 1: Effects of nitrogen and phosphorus sources on the accumulation of PHBHHx in activated sludge when carbon sources are added in stages

[0020] Use a 1L batch reactor, continuously aerate 2L / min from the aeration head, without stirring, and react for 16h at a temperature of 30°C. Under the condition that the total carbon source is kept at 1.5g / L sodium laurate, 0.5g sodium laurate is added at the 0th, 4th, and 8th hours, respectively. The concentration conditions of nitrogen source and phosphorus source in the four batch experiments are as follows: ( a) Nitrogen and phosphorus, NH 4 Cl, 0.16g / L+K 2 HPO 4 , 0.092g / L; (b) with nitrogen and phosphorus deficiency, NH 4 Cl, 0.16g / L+K 2 HPO 4 , 0g / L; (c) nitrogen deficiency with phosphorus, NH 4 Cl, 0g / L+K 2 HPO 4 , 0.092g / L; (d) nitrogen deficiency and phosphorus deficiency, NH 4 Cl, 0g / L+K 2 HPO 4 , 0g / L. from figure 1 It can be seen from (d) that the concentration of PHBHHx is the highest under the co...

Embodiment 2

[0021] Example 2: Influence of different aeration methods on the accumulation of PHBHHx in activated sludge under the condition of nitrogen deficiency and phosphorus deficiency

[0022] Using a 1L batch reactor, under the condition that the total carbon source is 1.5g / L sodium laurate, 0.5g sodium laurate was added at the 0th, 4th and 8th hours, respectively, for nitrogen deficiency and phosphorus deficiency, namely NH 4 Cl 0g / L+K 2 HPO 4 0g / L, reaction 16h, temperature 30 ℃. The aeration conditions in the four batch experiments were as follows: (a) continuous aeration; (b) 60min aeration + 30min stirring; (c) 60min aeration + 60min stirring; (d) 120min aeration + 120min stirring. Keep the aeration volume at 2L / min during aeration, and stop the aeration during stirring. from figure 2 It can be seen from (a) that the concentration of PHBHHx during continuous aeration is the highest under the conditions of the four batches. At this time, the concentrations of PHB, PHHx and ...

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Abstract

The invention discloses a method for producing medium-chain-length polyhydroxyalkanoates by virtue of excess activated sludge, and relates to polyhydroxyalkanoates. The method comprises the following steps: using sodium laurate as a unique substrate carbon source for domesticating original sludge, and synthesising poly-3-hydroxybutyrate-co-3-hydroxyhexanoate by virtue of the stably-domesticated activated sludge, wherein 1.5g/L sodium laurate is uniformly poured for three times while being used as the unique substrate, and the highest concentration of cumulative PHBHHx of the sludge is 505.5mg/L under a continuous aeration condition and in case of nitrogen deficiency and phosphorus deficiency; the PHBHHx are synthesised by virtue of activated sludge mixed micro-organisms for the first time, and an infrared absorption spectrum and thermogravimetric analysis proves that PHBHHx samples have chemical structures and physical properties similar to those of PHBHHx standard substances synthesised from pure strains. The method is simple in equipment, easy to operate, free from a sterile working condition and rich in excess sludge source, and therefore has a great application prospect in the aspect of producing plastic products needed in the industry and the agriculture.

Description

technical field [0001] The invention relates to polyhydroxyalkanoates, in particular to a method for producing medium and long-chain polyhydroxyalkanoates by using surplus activated sludge. Background technique [0002] Polyhydroxyalkanoates (PHAs) are a kind of high-carbohydrate synthesized by prokaryotic microorganisms that store excess carbon sources in the form of energy when they are limited by the supply of certain basic nutrients. Molecular Biopolyester. PHA has physicochemical properties similar to chemical plastics and unique biodegradability, and has the characteristics of complete biodegradability and biocompatibility that synthetic plastics do not have. It is a "green" polymer material. Therefore, The development of PHA series products has attracted more and more attention. [0003] PHA is formed from different hydroxyalkanoic acid monomers through thioester polymerization, such as poly-3-hydroxybutyrate-3-hydroxyhexanoate (poly(3-hydroxybutyrate-co-3-hydroxyhe...

Claims

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Application Information

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IPC IPC(8): C12P7/62
CPCC12P7/625
Inventor 王远鹏沈亮张传潘何宁李清彪卢英华
Owner XIAMEN UNIV
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