Visual DNA (deoxyribonucleic acid) chip kit and method for detecting multiple animal-derived components

A technology of animal-derived components and DNA chips, which is applied in biochemical equipment and methods, microbiological measurement/inspection, etc., can solve problems such as difficulty in primer selection, achieve accurate display of results, specific high-throughput, and reduce troubles Effect

Inactive Publication Date: 2015-04-29
珠海出入境检验检疫局检验检疫技术中心
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The shortcoming of the PCR method is that only a limited number of species can be detected at the same time, and it is difficult to select primers when the source background is unclear and it may be mixed with a variety of meat.

Method used

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  • Visual DNA (deoxyribonucleic acid) chip kit and method for detecting multiple animal-derived components
  • Visual DNA (deoxyribonucleic acid) chip kit and method for detecting multiple animal-derived components
  • Visual DNA (deoxyribonucleic acid) chip kit and method for detecting multiple animal-derived components

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Experimental program
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Effect test

Embodiment

[0031] 1 Materials and methods

[0032] 1.1 Materials

[0033] 1.1.1 Sample DNA source

[0034] Chicken, duck, goose, pork, beef, sheep and goat meat are samples preserved in this laboratory; one copy of Bengal tiger (white tiger variant) and Siberian tiger hair each come from Guangzhou Zoo; one bear paw is in this laboratory Preserved samples; one bear bile, from bear bile wine soaked for 2 years. The nucleic acid obtained from the above samples by DNA extraction method was used for PCR amplification. The leopard positive control is the mitochondrial Cytb gene, a cloning plasmid prepared in our laboratory.

[0035] 1.1.2 Main reagents and instruments

[0036] The DNA extraction kit E.Z.N.A.TM Tissue DNA Kit was a product of OMEGA Company in the United States; 2×Taq PCR Mastermix was a product of Tiangen Biochemical Technology (Beijing) Co., Ltd.; DNA Marker DL 2000 was purchased from Bao Biological Engineering (Dalian) Co., Ltd.

[0037] DR.chip DIY Kit (including chip s...

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Abstract

The invention discloses a visual DNA (deoxyribonucleic acid) chip kit and method for detecting multiple animal-derived components. The visual DNA chip detection method is used for animal derivation of chickens, ducks, geese, pigs, cattle, sheep, goats, tigers, bears and leopards. The method comprises the following steps: designing and synthesizing specific primers and probes according to the sequences of mitochondrial DNAs of the animals above in the GenBank; and carrying out chip sample application, hybridization and color development by using a Dr.chip system, optimizing reaction conditions, carrying out sensitivity and specificity testing and repetitive testing, and carrying out detection on the animal-derived components. The result indicates that the novel visual DNA chip detection method for chickens, ducks, geese, pigs, cattle, sheep, goats, tigers, bears and leopards has the characteristics of high sensitivity, high specificity and high flux.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a visual DNA chip kit and method for detecting various animal-derived components. Background technique [0002] With the rapid development of international trade, the difficulty of preventing the spread of international animal diseases is increasing. In order to prevent the spread of mad cow disease, scrapie, and animal flu through feed and animal products, governments and organizations around the world are actively carrying out research on animal-derived component detection technology while regulating the use of animal-derived feed. In addition, in domestic and international trade, cheap pork is mixed with beef and mutton, and shoddy deceitful behaviors are common, which brings a lot of inconvenience to the majority of Muslim friends and seriously damages the interests of consumers. Tigers, bears, and leopards are rare wild animals. Species identificatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/16
Inventor 罗宝正薄清如陶旻陈轩廖秀云沙才华徐海聂黄海超
Owner 珠海出入境检验检疫局检验检疫技术中心
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