Dark field digital holographic microscopy device based on vortex round airy light illumination and method using device

Abstract

The invention provides a dark field digital holographic microscopy device based on the vortex round airy light illumination and a method using the device. The device comprises a laser, a beam splitter prism I, a beam splitter prism II, a plane mirror, a microscope objective spatial filter, a microscope objective I, a Fourier lens, a diaphragm, a beam splitter prism III, a spatial light modulator, a dark field microscope objective, a sphere sample, a microscope objective II, a beam splitter prism IV and a photoelectric coupler. The implementation method comprises three steps: firstly, illuminating an object by using a ring-shaped light cone formed after the vortex round airy light enters the dark field microscope objective; then, recording the interference fringes of scattered light and reference light of the object into a computer through the photoelectric coupler by using a digital holography technology; finally, reconstructing the image of the object by using a digital reconstruction technology. Compared with a dark field digital holographic microscopy method based on vortex light illumination, the method, provided by the invention, has higher resolution ratio and contrast ratio, and is applicable to the research of the property of vortex round airy light beam in a laboratory, and is applicable to the fields of biomedicine, material science and the like.

Description

technical field [0001] The invention relates to a dark-field digital holographic technique for recording the interference fringes of scattered light of a sample and spherical wave reference light when illuminated by vortex circular Airy light, and specifically relates to a dark-field digital holographic display based on vortex circular Airy light illumination. The micro device and method belong to the technical field of digital holography. Background technique [0002] Digital holography technology is the product of the combination of computer technology and traditional optical holography. Using digital recording and reproduction methods, the amplitude information and phase information of the object can be obtained more conveniently, and the phase information is an important parameter for restoring the three-dimensional shape of the object. Digital holographic microscopic imaging is mainly carried out in two steps: first, pre-magnify the measured object with an optical micro...

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Problems solved by technology

However, although the vortex light has quasi-non-diffraction characteristics, the spot ring will still widen with the increase of the propagation distance during the propagation of the vortex light. At the same time, the spot energy will not be concentrated in the main ring. The band broadens and diffuses, which to some extent leads to a decrease in the resolution of the system and also increases the background noise

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