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Antiseptic agarose gel plate preparation method

A technology of agarose gel and agarose, which is applied in the field of medicine and health, and can solve the problems of short storage time, trouble, and easy growth of bacteria in agarose

Inactive Publication Date: 2015-05-06
SHANGHAI DIAN CLINICAL TESTING CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although great attention has been paid to the disinfection steps when preparing the gel plates, because the agarose is prone to bacteria growth, the time for the prepared agarose gel plates to be placed is very short, and often bacteria will grow and be scrapped in about a week; still need Do it now, it is more troublesome

Method used

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  • Antiseptic agarose gel plate preparation method
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  • Antiseptic agarose gel plate preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 made the same day experiment figure ( image 3 )

[0059] Take a fresh serum sample, use the preservative agarose gel plate made on the same day for protein electrophoresis experiment, and analyze the results:

[0060] swim lane ALB alpha 1 globulin alpha 2 globulin beta globulin gamma globulin 1 64.5 3.6 8.7 12.3 10.8 2 64.8 3.8 9.2 11.7 10.4 3 65.2 4.0 8.7 12.1 10.0 4 65.9 3.9 8.9 11.5 9.8 mean(x) 65.1 3.83 8.9 11.9 10.3 Standard Deviation(s) 0.52 0.15 0.20 0.32 0.38 Coefficient of Variation (CV%) 0.81 3.87 2.31 2.66 3.75

Embodiment 24

[0061] Example 24 ℃ refrigerator preservation 3 months sample experiment figure ( Figure 4 )

[0062] Take a sample and seal the antiseptic agarose gel plate with moisture in the box, moist appearance, sufficient moisture in the agar plate, plump, and no bacterial growth. Take a fresh serum sample, use the sample to do protein electrophoresis experiment, and analyze the results (because there is tailing in lane 4, the result is excluded):

[0063] swim lane ALB alpha 1 globulin alpha 2 globulin beta globulin gamma globulin 1 54.7 4.9 10.2 14.3 16.0 2 54.2 4.3 9.6 14.6 17.3 3 53.4 4.8 10.3 13.2 18.2 5 52.2 4.3 10.2 15.1 18.2 6 54.7 4.0 10.5 12.6 18.2 7 55.6 4.7 9.9 13.6 16.2 mean(x) 54.1 4.5 10.1 13.9 17.4 Standard Deviation(s) 1.19 0.35 0.32 0.93 1.03 Coefficient of Variation (CV%) 2.20 7.83 3.15 6.72 5.94

Embodiment 34

[0064] Example 34 ℃ refrigerator preservation 4 months sample experiment figure ( Figure 5 )

[0065] Take a sample and seal the antiseptic agarose gel plate with moisture in the box, moist appearance, sufficient moisture in the agar plate, plump, and no bacterial growth. Take a fresh serum sample, use the sample to do protein electrophoresis experiment, and analyze the results:

[0066] swim lane ALB alpha 1 globulin alpha 2 globulin beta globulin gamma globulin

[0067] 1 61.2 2.8 7.9 12.4 15.7 2 63.0 2.5 8.1 10.1 16.2 3 60.2 2.8 7.1 13.0 16.8 4 60.7 2.6 7.1 11.7 17.9 5 60.0 2.6 7.2 11.8 18.5 6 62.4 2.8 6.3 11.6 16.9 7 62.1 2.7 7.2 11.4 16.6 8 62.3 3.0 6.5 11.5 16.8 9 61.7 2.7 8.2 11.9 15.6 10 61.0 2.7 8.4 11.1 16.7 mean(x) 16.46 2.72 7.4 11.65 16.77 Standard Deviation(s) 1.00 0.14 0.72 0.76 0.89 Coefficient of Variation (CV%) 1.6...

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Abstract

The present invention discloses an antiseptic agarose gel plate preparation method, which comprises gel dissolving and plate preparing, wherein a mixed liquid of a gentamicin solution and a penicillin sodium solution is added after gel dissolving, and then plate preparing is performed. The antiseptic agarose gel plate preparation method concretely comprises: dissolving the gel, adding the mixed liquid of the gentamicin solution and the penicillin sodium solution, preparing the plate, and wrapping the prepared agarose gel plate with a sealed wrapping material. Compared with the antiseptic agarose gel plate preparation method in the prior art, the antiseptic agarose gel plate preparation method of the present invention has the following advantages that: the antibiotics are added during the agarose gel preparing process so as to achieve the antiseptic sterilization purpose and effectively solve the problem of short agarose gel plate storage time. The prepared antiseptic agarose gel plate of the present invention has advantages of small size, easy storage, strong antiseptic performance, and great market value.

Description

technical field [0001] The invention relates to the technical field of medicine and health, in particular to a method for preparing an antiseptic agarose gel plate. Background technique [0002] Agarose gel electrophoresis is an electrophoresis method that uses agarose as a support medium. In clinical application, for samples with larger molecular weight, agarose gel with larger pore size is generally used for electrophoresis separation. [0003] Many agarose molecules rely on hydrogen bonds and other forces to coil each other to form rope-like agarose bundles, forming a large mesh gel. Substance molecules will be resisted when they pass through, and the resistance of macromolecular substances is large when they are surging. Therefore, in gel electrophoresis, the separation of charged particles depends not only on the nature and quantity of the net charge, but also on the size of the molecule, which is Greatly improved resolution. Because there is no charged group on its ...

Claims

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Application Information

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IPC IPC(8): G01N27/453
Inventor 于嘉屏陆桂琴李艳梅王月婷
Owner SHANGHAI DIAN CLINICAL TESTING CENT
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