Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Three-dimensional parallel collagenous fiber-silk bracket as well as preparation method and application thereof

A collagen fiber and silk technology, which is applied in the field of three-dimensional parallel collagen fiber-silk scaffold and its preparation, can solve the problems of far away and achieve the effects of promoting differentiation, good biocompatibility and promoting regeneration

Active Publication Date: 2015-05-13
ZHEJIANG UNIV
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its structural arrangement is very different from the shape of tendon / ligament tissue, and the structure and function of repaired tendon / ligament are also very different from those of normal tendon / ligament

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Three-dimensional parallel collagenous fiber-silk bracket as well as preparation method and application thereof
  • Three-dimensional parallel collagenous fiber-silk bracket as well as preparation method and application thereof
  • Three-dimensional parallel collagenous fiber-silk bracket as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Set the mesh size to 1mm 2 woven silk with sericin removed, cut into 2*2cm (see figure 1 ). Inject 2mm thick 6mg / ml collagen below the braided silk, and inject 3mm thick 6mg / ml collagen above the braided silk. Adjust the distance between the foam box containing liquid nitrogen and the mold to be 5 cm. After solidification, place it in a vacuum drying machine to dry it, and then perform dry heat crosslinking to obtain a three-dimensional parallel collagen fiber-silk scaffold with a pore diameter of about 200um. Observe its scanning electron microscope picture (see Figure 3-1 ).

Embodiment 2

[0043] Set the mesh size to 1mm 2 The woven silk has sericin protein removed and cut into 2*2cm. 2mm thick 10mg / ml collagen was injected below the braided silk, and 3mm thick 10mg / ml collagen was injected above the braided silk. Adjust the distance between the foam box containing liquid nitrogen and the collagen and silk to 5 cm. After solidification, place it in a vacuum dehydrator to dry it, and then perform dry heat crosslinking to obtain a three-dimensional parallel collagen fiber-silk scaffold with a pore diameter of about 80um (see figure 2 ). Observe its scanning electron microscope picture (see Figure 3-2 ).

Embodiment 3

[0045] Set the mesh size to 1mm 2The woven silk has sericin protein removed and cut into 2*2cm. 2mm thick 18mg / ml collagen was injected below the braided silk, and 3mm thick 18mg / ml collagen was injected above the braided silk. Adjust the distance between the foam box containing liquid nitrogen and the collagen and silk to 5 cm. After solidification, place it in a vacuum drying machine to dry it, and then perform dry heat crosslinking to obtain a three-dimensional parallel collagen fiber-silk scaffold with a pore diameter of about 20um. Observe its scanning electron microscope picture (see Figure 3-3 ).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Meshaaaaaaaaaa
Pore diameteraaaaaaaaaa
Mesh sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides a three-dimensional parallel collagenous fiber-silk bracket as well as a preparation method thereof. The bracket is formed by a woven silk base frame and a parallel collagenous fiber layer which coats on the upper and lower surfaces of the woven silk base frame, wherein the woven silk base frame adopts a mesh structure which is woven by silk and has a mesh size of 0.25-25 mm<2>; the pore diameter of the parallel collagenous fiber layer is 10-300 microns; the woven silk is sericin-removed woven silk; the bracket can be used as a biological bracket and applied to the repair of muscle tendons / ligaments. The three-dimensional parallel collagenous fiber-silk bracket provided by the invention is simple to prepare, good in biocompatibility and good in mechanical property, has a topological structure similar to muscle tendons / ligaments and can be used for promoting the surface grown stem cells to differentiate toward the muscle tendons / ligaments and promoting the regeneration of the muscle tendons / ligaments.

Description

(1) Technical field [0001] The invention relates to a three-dimensional support material for tendon / ligament repair and a preparation method thereof, in particular to a three-dimensional parallel collagen fiber-silk support and a preparation method and application thereof. (2) Background technology [0002] With the development of our country's economy and the improvement of people's living standards, people's health concepts are also improving. Out of yearning for a healthy body, the number of Chinese people participating in physical exercises and competitions has increased significantly, and sports injuries have also increased. Among them Ligament / tendon injuries account for more than 50%. Statistics show that there are at least tens of millions of tendon injuries per 200 million people a year. [0003] Currently, ligament and tendon injuries are mainly repaired clinically by direct suture, autograft, allograft, xenograft, and prosthetic material repair. However, these s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/24A61L27/36
Inventor 欧阳宏伟沈炜亮郑泽峰陈晓
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com