Method for preparing carbon dots based on carbon-printed electrodes on chip

A carbon quantum dot and carbon printing technology, which is applied in the field of carbon quantum dot preparation based on carbon printing electrodes on a chip, and achieves the effects of simple device, remarkable imaging effect and low dosage.

Inactive Publication Date: 2015-05-13
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there have been some reports on the electrolytic buffer that can be used to generate carbon quantum dots by electrochemical methods, such as ionic liquid aqueous solution, ethanol-doped NaOH solution, etc., wherein the ionic liquid aqueous solution is used as an electrolytic buffer to produce carbon quantum dots. The efficiency is high and it is easy to repeat. At the same time, ionic liquid-functionalized carbon quantum dots can be obtained, but so far there has been no report of ionic liquid-functionalized carbon quantum dots being applied to cell imaging; in addition, some carbon quantum dots have been disclosed to have catalytic or amplification properties for many electrochemiluminescent systems. However, there are no reports on the application of carbon quantum dots to the development of solid-state electrochemiluminescent sensors on paper chips. Therefore, there are still many new application fields for carbon quantum dots to be developed.

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  • Method for preparing carbon dots based on carbon-printed electrodes on chip
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  • Method for preparing carbon dots based on carbon-printed electrodes on chip

Examples

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Embodiment 1

[0020] The process for preparing carbon quantum dots in this embodiment specifically includes the following steps:

[0021] (1), make the two-electrode system of carbon printing electrode on the paper chip by existing screen printing technology, as positive pole and negative pole in the electrochemical method;

[0022] (2), 15-50 μ L of electrolyte solution 3 is placed in the electrolytic cell 2 on the paper chip 1, the power supply 6 is turned on, and a DC constant voltage of 6.0 V is applied across the positive electrode 4 and the negative electrode 5 on the paper chip 1 to react 10- The carbon quantum dot solution was obtained in 30 minutes. After the reaction, the current was stable, and the electrolyte 3 changed from transparent to dark brown, and did not change;

[0023] (3) The carbon quantum dot solution prepared in step (2) is centrifuged at 12000-16000 rpm for 10-30 min to remove solid impurities, and the supernatant is obtained as the prepared carbon quantum dot.

Embodiment 2

[0025] In this embodiment, the carbon quantum dot solution prepared in Example 1 is applied to cell imaging, and HeLa cells (10 6 cells / sample) placed on a glass slide with a size of 35mm, prepare a fresh DMEM solution containing 1 / 10 of the carbon quantum dot solution concentration, and incubate HeLa cells with the prepared DMEM solution at 37°C for 1h to obtain incubated cells; then at room temperature, All the incubated cells were washed three times with phosphate buffer solution to remove excess unabsorbed carbon quantum dots, and the 488nm Ar ion laser was selected to excite the incubated cells, and the images of the incubated cells were collected by a LEICATCS SP2 laser scanning confocal microscope, and further collected The imaging of cells in a fixed area over time, image 3 In A, the left image is a fluorescence image, and the right image is a bright field image, which shows that the cell imaging effect is remarkable.

[0026] In this example, the cytotoxicity was te...

Embodiment 3

[0029] In this embodiment, the carbon quantum dot solution prepared in Example 1 is applied to the application of enhanced electrochemiluminescence, and the CH800 voltammetry analyzer is used to carry out the voltammetry curve scanning experiment, and the MPI-A type capillary electrophoresis electrochemiluminescence detector (Xi'an Ruimai Analytical Instrument Co., Ltd., Xi’an, China) detects the intensity of the electrochemiluminescence signal. During the detection process, the voltage of the photomultiplier tube (PMT) is set to 800V to collect the electrochemiluminescence signal. First, the carbon electrode is printed on the chip substrate. Form the whole chip system for electrochemiluminescence detection, select the cation exchange membrane Nafion to build a solid-state electrochemiluminescence sensor, first take 20 μL of carbon quantum dot solution and disperse it in the same volume of 1.0wt% Nafion solution and ultrasonically obtain a uniform and well-dispersed Nafion / CNDs...

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Abstract

The invention belongs to the technical field of manufacture of carbon nanomaterials, and relates to a method for preparing carbon dots based on carbon-printed electrodes on a chip. The method comprises the steps of manufacturing two electrode systems of the carbon-printed electrodes on the chip as a positive electrode and a negative electrode in an electrochemical method; placing electrolyte into an electrolytic tank on the chip, turning on the power, applying direct-current constant voltage on two ends of the positive electrode and the negative electrode on the chip for reaction to obtain a carbon dot solution; centrifuging the prepared carbon-dot solution by a centrifugal machine to remove solid impurities, and taking supernatant liquor as the prepared carbon dots. The method for preparing the carbon dots is simple, the theory is scientific, the cost is low, the efficiency is high, and the prepared carbon dots are high in quality, stable in performance and wide in application.

Description

Technical field: [0001] The invention belongs to the technical field of carbon nanomaterial preparation, and relates to a process for preparing carbon quantum dots by an electrochemical method, in particular to a portable method for preparing carbon quantum dots based on carbon printing electrodes on a chip, and applying the prepared carbon quantum dots to to bioimaging and enhanced electrochemiluminescence. Background technique: [0002] Carbon quantum dots are a new type of carbon material, which has excellent luminescent properties and small size characteristics (generally less than 10nm) similar to traditional semiconductor quantum dots. It also has many advantages such as rich raw materials, low toxicity, and biocompatibility. Imaging, drug carrier, sensor preparation, optics, catalysis and other fields have great application value. At present, there are many reports on the preparation of carbon quantum dots, mainly including laser exfoliation of graphite, arc discharg...

Claims

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Application Information

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IPC IPC(8): C25B1/00G01N21/76
CPCG01N21/76C25B1/00
Inventor 许元红刘敬权宗西丹
Owner QINGDAO UNIV
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