Method for rapidly determining content of 5-HMF (hydroxymethylfurfural) in glucose dehydration reaction liquid
A technology of hydroxymethylfurfural and dehydration reaction, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of gas chromatograph and high performance liquid chromatograph damage, cumbersome operation steps, long detection time, etc., and achieve accurate and reliable measurement results , The operation process is simple and the detection time is short
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Embodiment 1
[0023] Embodiment 1: Determination of 5-hydroxymethylfurfural content in the glucose dehydration reaction solution according to the following steps
[0024] (a) Accurately prepare 0.010 mg / mL HMF standard solution and dilute it to 0.001, 0.002, 0.004, 0.006 and 0.008 mg / mL respectively, and perform full-band spectral scanning on the ultraviolet spectrophotometer respectively. The scanning results are as follows: figure 1 shown; from figure 1 It can be seen that, under different concentrations, the maximum ultraviolet absorption peak of HMF is at 284 nm, therefore, determine that 284 nm is the maximum absorption wavelength of HMF;
[0025] (b) Prepare 0.01 mg / mL glucose standard solution and 0.1 mg / mL [BMIM]Cl standard solution respectively, and perform full-band spectral scanning on the ultraviolet spectrophotometer respectively. The scanning results are as follows: figure 2 shown; from figure 2 It can be seen that glucose has no UV absorption in the wavelength range of 20...
Embodiment 2
[0037] Example 2: Step (a-c) is the same as embodiment 1; then ， 100 mg glucose and 6.9 mg CrCl 2 Add it to 1 g of [BMIM]Cl, seal it, place it in an oil bath and heat it at 120 °C for 30 min; after the reaction is completed, quickly cool down to room temperature in an ice-water bath, add 4 mL of deionized water, and mix well Centrifuge at 10000 rpm for 5 min, and the upper layer is the glucose dehydration reaction solution; the above-mentioned glucose dehydration reaction solution is diluted 2000 times, and its absorbance is measured with a UV spectrophotometer, and the HMF content calculated according to the standard curve in Example 1 is 46.21 mg.
Embodiment 3
[0038] Example 3: Steps (a-c) are the same as in Example 1; then, 100 mg glucose and 14.9 mg CrCl 3 ?6H 2O was added to 1 g [BMIM]Cl, sealed and placed in an oil bath and heated at 120 °C for 30 min; after the reaction was completed, quickly cooled to room temperature in an ice-water bath, added 4 mL of deionized water, and mixed Uniform; centrifuge at 5000 rpm for 10 min, and the upper layer is the glucose dehydration reaction solution; dilute the above glucose dehydration reaction solution 2000 times, measure its absorbance with an ultraviolet spectrophotometer, and calculate the HMF content according to the standard curve in Example 1 to be 50.73 mg .
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